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Phylogeography and conservation gaps of Musa balbisiana Colla genetic diversity revealed by microsatellite markers

Collection and storage of crop wild relative (CWR) germplasm is crucial for preserving species genetic diversity and crop improvement. Nevertheless, much of the genetic variation of CWRs is absent in ex situ collections and detailed passport data are often lacking. Here, we focussed on Musa balbisia...

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Autores principales: Mertens, Arne, Bawin, Yves, Vanden Abeele, Samuel, Kallow, Simon, Swennen, Rony, Vu, Dang Toan, Vu, Tuong Dang, Minh, Ho Thi, Panis, Bart, Vandelook, Filip, Janssens, Steven B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393128/
https://www.ncbi.nlm.nih.gov/pubmed/36017134
http://dx.doi.org/10.1007/s10722-022-01389-4
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author Mertens, Arne
Bawin, Yves
Vanden Abeele, Samuel
Kallow, Simon
Swennen, Rony
Vu, Dang Toan
Vu, Tuong Dang
Minh, Ho Thi
Panis, Bart
Vandelook, Filip
Janssens, Steven B.
author_facet Mertens, Arne
Bawin, Yves
Vanden Abeele, Samuel
Kallow, Simon
Swennen, Rony
Vu, Dang Toan
Vu, Tuong Dang
Minh, Ho Thi
Panis, Bart
Vandelook, Filip
Janssens, Steven B.
author_sort Mertens, Arne
collection PubMed
description Collection and storage of crop wild relative (CWR) germplasm is crucial for preserving species genetic diversity and crop improvement. Nevertheless, much of the genetic variation of CWRs is absent in ex situ collections and detailed passport data are often lacking. Here, we focussed on Musa balbisiana, one of the two main progenitor species of many banana cultivars. We investigated the genetic structure of M. balbisiana across its distribution range using microsatellite markers. Accessions stored at the International Musa Germplasm Transit Centre (ITC) ex situ collection were compared with plant material collected from multiple countries and home gardens from Vietnam. Genetic structure analyses revealed that accessions could be divided into three main clusters. Vietnamese and Chinese populations were assigned to a first and second cluster respectively. A third cluster consisted of ITC and home garden accessions. Samples from Papua New Guinea were allocated to the cluster with Chinese populations but were assigned to a separate fourth cluster if the number of allowed clusters was set higher. Only one ITC accession grouped with native M. balbisiana populations and one group of ITC accessions was nearly genetically identical to home garden samples. This questioned their wild status, including accessions used as reference for wild M. balbisiana. Moreover, most ITC accessions and home garden samples were genetically distinct from wild populations. Our results highlight that additional germplasm should be collected from the native distribution range, especially from Northeast India, Myanmar, China, and the Philippines and stored for ex situ conservation at the ITC. The lack of passport data for many M. balbisiana accessions also complicates the interpretation of genetic information in relation to cultivation and historical dispersal routes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10722-022-01389-4.
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spelling pubmed-93931282022-08-23 Phylogeography and conservation gaps of Musa balbisiana Colla genetic diversity revealed by microsatellite markers Mertens, Arne Bawin, Yves Vanden Abeele, Samuel Kallow, Simon Swennen, Rony Vu, Dang Toan Vu, Tuong Dang Minh, Ho Thi Panis, Bart Vandelook, Filip Janssens, Steven B. Genet Resour Crop Evol Research Article Collection and storage of crop wild relative (CWR) germplasm is crucial for preserving species genetic diversity and crop improvement. Nevertheless, much of the genetic variation of CWRs is absent in ex situ collections and detailed passport data are often lacking. Here, we focussed on Musa balbisiana, one of the two main progenitor species of many banana cultivars. We investigated the genetic structure of M. balbisiana across its distribution range using microsatellite markers. Accessions stored at the International Musa Germplasm Transit Centre (ITC) ex situ collection were compared with plant material collected from multiple countries and home gardens from Vietnam. Genetic structure analyses revealed that accessions could be divided into three main clusters. Vietnamese and Chinese populations were assigned to a first and second cluster respectively. A third cluster consisted of ITC and home garden accessions. Samples from Papua New Guinea were allocated to the cluster with Chinese populations but were assigned to a separate fourth cluster if the number of allowed clusters was set higher. Only one ITC accession grouped with native M. balbisiana populations and one group of ITC accessions was nearly genetically identical to home garden samples. This questioned their wild status, including accessions used as reference for wild M. balbisiana. Moreover, most ITC accessions and home garden samples were genetically distinct from wild populations. Our results highlight that additional germplasm should be collected from the native distribution range, especially from Northeast India, Myanmar, China, and the Philippines and stored for ex situ conservation at the ITC. The lack of passport data for many M. balbisiana accessions also complicates the interpretation of genetic information in relation to cultivation and historical dispersal routes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10722-022-01389-4. Springer Netherlands 2022-05-07 2022 /pmc/articles/PMC9393128/ /pubmed/36017134 http://dx.doi.org/10.1007/s10722-022-01389-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Mertens, Arne
Bawin, Yves
Vanden Abeele, Samuel
Kallow, Simon
Swennen, Rony
Vu, Dang Toan
Vu, Tuong Dang
Minh, Ho Thi
Panis, Bart
Vandelook, Filip
Janssens, Steven B.
Phylogeography and conservation gaps of Musa balbisiana Colla genetic diversity revealed by microsatellite markers
title Phylogeography and conservation gaps of Musa balbisiana Colla genetic diversity revealed by microsatellite markers
title_full Phylogeography and conservation gaps of Musa balbisiana Colla genetic diversity revealed by microsatellite markers
title_fullStr Phylogeography and conservation gaps of Musa balbisiana Colla genetic diversity revealed by microsatellite markers
title_full_unstemmed Phylogeography and conservation gaps of Musa balbisiana Colla genetic diversity revealed by microsatellite markers
title_short Phylogeography and conservation gaps of Musa balbisiana Colla genetic diversity revealed by microsatellite markers
title_sort phylogeography and conservation gaps of musa balbisiana colla genetic diversity revealed by microsatellite markers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393128/
https://www.ncbi.nlm.nih.gov/pubmed/36017134
http://dx.doi.org/10.1007/s10722-022-01389-4
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