Cargando…
Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species
Coccidiosis caused by the Eimeria species is a highly problematic disease in the chicken industry. Here, we used RNA sequencing to observe the time-dependent host responses of Eimeria-infected chickens to examine the genes and biological functions associated with immunity to the parasite. Transcript...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393255/ https://www.ncbi.nlm.nih.gov/pubmed/36003329 http://dx.doi.org/10.3389/fgene.2022.886781 |
_version_ | 1784771231248023552 |
---|---|
author | Kim, Minjun Chung, Yoonji Manjula, Prabuddha Seo, Dongwon Cho, Sunghyun Cho, Eunjin Ediriweera, Thisarani Kalhari Yu, Myunghwan Nam, Sunju Lee, Jun Heon |
author_facet | Kim, Minjun Chung, Yoonji Manjula, Prabuddha Seo, Dongwon Cho, Sunghyun Cho, Eunjin Ediriweera, Thisarani Kalhari Yu, Myunghwan Nam, Sunju Lee, Jun Heon |
author_sort | Kim, Minjun |
collection | PubMed |
description | Coccidiosis caused by the Eimeria species is a highly problematic disease in the chicken industry. Here, we used RNA sequencing to observe the time-dependent host responses of Eimeria-infected chickens to examine the genes and biological functions associated with immunity to the parasite. Transcriptome analysis was performed at three time points: 4, 7, and 21 days post-infection (dpi). Based on the changes in gene expression patterns, we defined three groups of genes that showed differential expression. This enabled us to capture evidence of endoplasmic reticulum stress at the initial stage of Eimeria infection. Furthermore, we found that innate immune responses against the parasite were activated at the first exposure; they then showed gradual normalization. Although the cytokine-cytokine receptor interaction pathway was significantly operative at 4 dpi, its downregulation led to an anti-inflammatory effect. Additionally, the construction of gene co-expression networks enabled identification of immunoregulation hub genes and critical pattern recognition receptors after Eimeria infection. Our results provide a detailed understanding of the host-pathogen interaction between chicken and Eimeria. The clusters of genes defined in this study can be utilized to improve chickens for coccidiosis control. |
format | Online Article Text |
id | pubmed-9393255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93932552022-08-23 Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species Kim, Minjun Chung, Yoonji Manjula, Prabuddha Seo, Dongwon Cho, Sunghyun Cho, Eunjin Ediriweera, Thisarani Kalhari Yu, Myunghwan Nam, Sunju Lee, Jun Heon Front Genet Genetics Coccidiosis caused by the Eimeria species is a highly problematic disease in the chicken industry. Here, we used RNA sequencing to observe the time-dependent host responses of Eimeria-infected chickens to examine the genes and biological functions associated with immunity to the parasite. Transcriptome analysis was performed at three time points: 4, 7, and 21 days post-infection (dpi). Based on the changes in gene expression patterns, we defined three groups of genes that showed differential expression. This enabled us to capture evidence of endoplasmic reticulum stress at the initial stage of Eimeria infection. Furthermore, we found that innate immune responses against the parasite were activated at the first exposure; they then showed gradual normalization. Although the cytokine-cytokine receptor interaction pathway was significantly operative at 4 dpi, its downregulation led to an anti-inflammatory effect. Additionally, the construction of gene co-expression networks enabled identification of immunoregulation hub genes and critical pattern recognition receptors after Eimeria infection. Our results provide a detailed understanding of the host-pathogen interaction between chicken and Eimeria. The clusters of genes defined in this study can be utilized to improve chickens for coccidiosis control. Frontiers Media S.A. 2022-08-08 /pmc/articles/PMC9393255/ /pubmed/36003329 http://dx.doi.org/10.3389/fgene.2022.886781 Text en Copyright © 2022 Kim, Chung, Manjula, Seo, Cho, Cho, Ediriweera, Yu, Nam and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Kim, Minjun Chung, Yoonji Manjula, Prabuddha Seo, Dongwon Cho, Sunghyun Cho, Eunjin Ediriweera, Thisarani Kalhari Yu, Myunghwan Nam, Sunju Lee, Jun Heon Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species |
title | Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species |
title_full | Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species |
title_fullStr | Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species |
title_full_unstemmed | Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species |
title_short | Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species |
title_sort | time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed eimeria species |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393255/ https://www.ncbi.nlm.nih.gov/pubmed/36003329 http://dx.doi.org/10.3389/fgene.2022.886781 |
work_keys_str_mv | AT kimminjun timeseriestranscriptomeanalysisidentifieddifferentiallyexpressedgenesinbroilerchickeninfectedwithmixedeimeriaspecies AT chungyoonji timeseriestranscriptomeanalysisidentifieddifferentiallyexpressedgenesinbroilerchickeninfectedwithmixedeimeriaspecies AT manjulaprabuddha timeseriestranscriptomeanalysisidentifieddifferentiallyexpressedgenesinbroilerchickeninfectedwithmixedeimeriaspecies AT seodongwon timeseriestranscriptomeanalysisidentifieddifferentiallyexpressedgenesinbroilerchickeninfectedwithmixedeimeriaspecies AT chosunghyun timeseriestranscriptomeanalysisidentifieddifferentiallyexpressedgenesinbroilerchickeninfectedwithmixedeimeriaspecies AT choeunjin timeseriestranscriptomeanalysisidentifieddifferentiallyexpressedgenesinbroilerchickeninfectedwithmixedeimeriaspecies AT ediriweerathisaranikalhari timeseriestranscriptomeanalysisidentifieddifferentiallyexpressedgenesinbroilerchickeninfectedwithmixedeimeriaspecies AT yumyunghwan timeseriestranscriptomeanalysisidentifieddifferentiallyexpressedgenesinbroilerchickeninfectedwithmixedeimeriaspecies AT namsunju timeseriestranscriptomeanalysisidentifieddifferentiallyexpressedgenesinbroilerchickeninfectedwithmixedeimeriaspecies AT leejunheon timeseriestranscriptomeanalysisidentifieddifferentiallyexpressedgenesinbroilerchickeninfectedwithmixedeimeriaspecies |