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Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species

Coccidiosis caused by the Eimeria species is a highly problematic disease in the chicken industry. Here, we used RNA sequencing to observe the time-dependent host responses of Eimeria-infected chickens to examine the genes and biological functions associated with immunity to the parasite. Transcript...

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Autores principales: Kim, Minjun, Chung, Yoonji, Manjula, Prabuddha, Seo, Dongwon, Cho, Sunghyun, Cho, Eunjin, Ediriweera, Thisarani Kalhari, Yu, Myunghwan, Nam, Sunju, Lee, Jun Heon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393255/
https://www.ncbi.nlm.nih.gov/pubmed/36003329
http://dx.doi.org/10.3389/fgene.2022.886781
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author Kim, Minjun
Chung, Yoonji
Manjula, Prabuddha
Seo, Dongwon
Cho, Sunghyun
Cho, Eunjin
Ediriweera, Thisarani Kalhari
Yu, Myunghwan
Nam, Sunju
Lee, Jun Heon
author_facet Kim, Minjun
Chung, Yoonji
Manjula, Prabuddha
Seo, Dongwon
Cho, Sunghyun
Cho, Eunjin
Ediriweera, Thisarani Kalhari
Yu, Myunghwan
Nam, Sunju
Lee, Jun Heon
author_sort Kim, Minjun
collection PubMed
description Coccidiosis caused by the Eimeria species is a highly problematic disease in the chicken industry. Here, we used RNA sequencing to observe the time-dependent host responses of Eimeria-infected chickens to examine the genes and biological functions associated with immunity to the parasite. Transcriptome analysis was performed at three time points: 4, 7, and 21 days post-infection (dpi). Based on the changes in gene expression patterns, we defined three groups of genes that showed differential expression. This enabled us to capture evidence of endoplasmic reticulum stress at the initial stage of Eimeria infection. Furthermore, we found that innate immune responses against the parasite were activated at the first exposure; they then showed gradual normalization. Although the cytokine-cytokine receptor interaction pathway was significantly operative at 4 dpi, its downregulation led to an anti-inflammatory effect. Additionally, the construction of gene co-expression networks enabled identification of immunoregulation hub genes and critical pattern recognition receptors after Eimeria infection. Our results provide a detailed understanding of the host-pathogen interaction between chicken and Eimeria. The clusters of genes defined in this study can be utilized to improve chickens for coccidiosis control.
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spelling pubmed-93932552022-08-23 Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species Kim, Minjun Chung, Yoonji Manjula, Prabuddha Seo, Dongwon Cho, Sunghyun Cho, Eunjin Ediriweera, Thisarani Kalhari Yu, Myunghwan Nam, Sunju Lee, Jun Heon Front Genet Genetics Coccidiosis caused by the Eimeria species is a highly problematic disease in the chicken industry. Here, we used RNA sequencing to observe the time-dependent host responses of Eimeria-infected chickens to examine the genes and biological functions associated with immunity to the parasite. Transcriptome analysis was performed at three time points: 4, 7, and 21 days post-infection (dpi). Based on the changes in gene expression patterns, we defined three groups of genes that showed differential expression. This enabled us to capture evidence of endoplasmic reticulum stress at the initial stage of Eimeria infection. Furthermore, we found that innate immune responses against the parasite were activated at the first exposure; they then showed gradual normalization. Although the cytokine-cytokine receptor interaction pathway was significantly operative at 4 dpi, its downregulation led to an anti-inflammatory effect. Additionally, the construction of gene co-expression networks enabled identification of immunoregulation hub genes and critical pattern recognition receptors after Eimeria infection. Our results provide a detailed understanding of the host-pathogen interaction between chicken and Eimeria. The clusters of genes defined in this study can be utilized to improve chickens for coccidiosis control. Frontiers Media S.A. 2022-08-08 /pmc/articles/PMC9393255/ /pubmed/36003329 http://dx.doi.org/10.3389/fgene.2022.886781 Text en Copyright © 2022 Kim, Chung, Manjula, Seo, Cho, Cho, Ediriweera, Yu, Nam and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Kim, Minjun
Chung, Yoonji
Manjula, Prabuddha
Seo, Dongwon
Cho, Sunghyun
Cho, Eunjin
Ediriweera, Thisarani Kalhari
Yu, Myunghwan
Nam, Sunju
Lee, Jun Heon
Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species
title Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species
title_full Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species
title_fullStr Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species
title_full_unstemmed Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species
title_short Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed Eimeria species
title_sort time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixed eimeria species
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393255/
https://www.ncbi.nlm.nih.gov/pubmed/36003329
http://dx.doi.org/10.3389/fgene.2022.886781
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