Cargando…
Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis
BACKGROUND AND PURPOSE: The diagnosis of tuberculous meningitis (TBM) is difficult due to the lack of sensitive methods. Identification of TBM-specific biomarkers in the cerebrospinal fluid (CSF) may help diagnose and improve our understanding of TBM pathogenesis. PATIENTS AND METHODS: Of the 112 su...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393334/ https://www.ncbi.nlm.nih.gov/pubmed/36003300 http://dx.doi.org/10.3389/fneur.2022.886040 |
_version_ | 1784771250491490304 |
---|---|
author | Huang, Mailing Ding, Zeyu Li, Wensheng Chen, Weibi Du, Yadong Jia, Hongyan Sun, Qi Du, Boping Wei, Rongrong Xing, Aiying Li, Qi Chu, Naihui Pan, Liping |
author_facet | Huang, Mailing Ding, Zeyu Li, Wensheng Chen, Weibi Du, Yadong Jia, Hongyan Sun, Qi Du, Boping Wei, Rongrong Xing, Aiying Li, Qi Chu, Naihui Pan, Liping |
author_sort | Huang, Mailing |
collection | PubMed |
description | BACKGROUND AND PURPOSE: The diagnosis of tuberculous meningitis (TBM) is difficult due to the lack of sensitive methods. Identification of TBM-specific biomarkers in the cerebrospinal fluid (CSF) may help diagnose and improve our understanding of TBM pathogenesis. PATIENTS AND METHODS: Of the 112 suspected patients with TBM prospectively enrolled in the study, 32 patients with inconclusive diagnosis, non-infectious meningitis, and long-term treatment with hormones and immunosuppressants were excluded. The expression of 8 proteins in the CSF was analyzed using ELISA in 22 patients with definite TBM, 18 patients with probable TBM, and 40 patients with non-TBM. RESULTS: Significant differences in the expression of 7 proteins were detected between the TBM and non-TBM groups (P < 0.01). Unsupervised hierarchical clustering (UHC) analysis revealed a disease-specific profile consisting of 7 differentially expressed proteins for TBM diagnosis, with an accuracy of 82.5% (66/80). Logistic regression with forward stepwise analysis indicated that a combination of 3 biomarkers (APOE_APOAI_S100A8) showed a better ability to discriminate TBM from patients with non-TBM [area under the curve (AUC) = 0.916 (95%CI: 0.857–0.976)], with a sensitivity of 95.0% (95%CI: 83.1–99.4%) and a specificity of 77.5% (95%CI: 61.5–89.2%). CONCLUSION: Our results confirmed the potential ability of CSF proteins to distinguish TBM from patients with non-TBM and provided a useful panel for the diagnosis of TBM. |
format | Online Article Text |
id | pubmed-9393334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93933342022-08-23 Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis Huang, Mailing Ding, Zeyu Li, Wensheng Chen, Weibi Du, Yadong Jia, Hongyan Sun, Qi Du, Boping Wei, Rongrong Xing, Aiying Li, Qi Chu, Naihui Pan, Liping Front Neurol Neurology BACKGROUND AND PURPOSE: The diagnosis of tuberculous meningitis (TBM) is difficult due to the lack of sensitive methods. Identification of TBM-specific biomarkers in the cerebrospinal fluid (CSF) may help diagnose and improve our understanding of TBM pathogenesis. PATIENTS AND METHODS: Of the 112 suspected patients with TBM prospectively enrolled in the study, 32 patients with inconclusive diagnosis, non-infectious meningitis, and long-term treatment with hormones and immunosuppressants were excluded. The expression of 8 proteins in the CSF was analyzed using ELISA in 22 patients with definite TBM, 18 patients with probable TBM, and 40 patients with non-TBM. RESULTS: Significant differences in the expression of 7 proteins were detected between the TBM and non-TBM groups (P < 0.01). Unsupervised hierarchical clustering (UHC) analysis revealed a disease-specific profile consisting of 7 differentially expressed proteins for TBM diagnosis, with an accuracy of 82.5% (66/80). Logistic regression with forward stepwise analysis indicated that a combination of 3 biomarkers (APOE_APOAI_S100A8) showed a better ability to discriminate TBM from patients with non-TBM [area under the curve (AUC) = 0.916 (95%CI: 0.857–0.976)], with a sensitivity of 95.0% (95%CI: 83.1–99.4%) and a specificity of 77.5% (95%CI: 61.5–89.2%). CONCLUSION: Our results confirmed the potential ability of CSF proteins to distinguish TBM from patients with non-TBM and provided a useful panel for the diagnosis of TBM. Frontiers Media S.A. 2022-08-08 /pmc/articles/PMC9393334/ /pubmed/36003300 http://dx.doi.org/10.3389/fneur.2022.886040 Text en Copyright © 2022 Huang, Ding, Li, Chen, Du, Jia, Sun, Du, Wei, Xing, Li, Chu and Pan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neurology Huang, Mailing Ding, Zeyu Li, Wensheng Chen, Weibi Du, Yadong Jia, Hongyan Sun, Qi Du, Boping Wei, Rongrong Xing, Aiying Li, Qi Chu, Naihui Pan, Liping Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis |
title | Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis |
title_full | Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis |
title_fullStr | Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis |
title_full_unstemmed | Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis |
title_short | Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis |
title_sort | identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis |
topic | Neurology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393334/ https://www.ncbi.nlm.nih.gov/pubmed/36003300 http://dx.doi.org/10.3389/fneur.2022.886040 |
work_keys_str_mv | AT huangmailing identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT dingzeyu identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT liwensheng identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT chenweibi identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT duyadong identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT jiahongyan identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT sunqi identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT duboping identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT weirongrong identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT xingaiying identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT liqi identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT chunaihui identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis AT panliping identificationofproteinbiomarkersinhostcerebrospinalfluidfordifferentialdiagnosisoftuberculousmeningitisandothermeningitis |