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Investigation of the Antinociceptive Activity of the Hydroethanolic Extract of Junglas nigra Leaf by the Tail-Immersion and Formalin Pain Tests in Rats
BACKGROUND: Juglans (J.) nigra leaf is obtained from a plant that is used in traditional medicine in some countries to alleviate inflammatory diseases. AIM: The aim of this study was to compare the effects of J. nigra extract on acute nociceptive and inflammatory pain in rats. METHODS: Antinocicepti...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393677/ https://www.ncbi.nlm.nih.gov/pubmed/36003322 http://dx.doi.org/10.1177/15593258221119877 |
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author | Srebro, Dragana Rajković, Katarina Dožić, Branko Vujović, Katarina Savić Brkić, Branislava Medić Milić, Petar Vučković, Sonja |
author_facet | Srebro, Dragana Rajković, Katarina Dožić, Branko Vujović, Katarina Savić Brkić, Branislava Medić Milić, Petar Vučković, Sonja |
author_sort | Srebro, Dragana |
collection | PubMed |
description | BACKGROUND: Juglans (J.) nigra leaf is obtained from a plant that is used in traditional medicine in some countries to alleviate inflammatory diseases. AIM: The aim of this study was to compare the effects of J. nigra extract on acute nociceptive and inflammatory pain in rats. METHODS: Antinociceptive activity was examined in Wistar rats by the tail-immersion and formalin tests. Motor function was assessed using the rotarod test. Plant extract was administered intraperitoneally. RESULTS: In the tail-immersion test, the maximal antinociceptive effect of the plant extract (100–330 mg/kg) was about 24–30% and is the result of the effect of a high concentration of ethanol. In the formalin test, the plant extract (41.3–330 mg/kg) significantly and dose-dependently inhibited nociception in both phases of the test with similar maximal effects of about 76% and 85%. Only the plant extract at the dose of 330 mg/kg caused a significant time-dependent reduction in time spent on the rotarod. CONCLUSIONS: In rats, the preventive systemic administration of the hydroethanolic extract of J. nigra leaf reduced chemically but not thermally induced pain. Higher efficacy was obtained in pain associated with inflammation and tissue injury. The antinociceptive effect is dose-dependent and may be limited by motor impairment. |
format | Online Article Text |
id | pubmed-9393677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-93936772022-08-23 Investigation of the Antinociceptive Activity of the Hydroethanolic Extract of Junglas nigra Leaf by the Tail-Immersion and Formalin Pain Tests in Rats Srebro, Dragana Rajković, Katarina Dožić, Branko Vujović, Katarina Savić Brkić, Branislava Medić Milić, Petar Vučković, Sonja Dose Response Inflammatory Parameters as Early Biomarkers in Prediction and Monitoring of Therapeutic Effects in Preclinical and Clinical Studies BACKGROUND: Juglans (J.) nigra leaf is obtained from a plant that is used in traditional medicine in some countries to alleviate inflammatory diseases. AIM: The aim of this study was to compare the effects of J. nigra extract on acute nociceptive and inflammatory pain in rats. METHODS: Antinociceptive activity was examined in Wistar rats by the tail-immersion and formalin tests. Motor function was assessed using the rotarod test. Plant extract was administered intraperitoneally. RESULTS: In the tail-immersion test, the maximal antinociceptive effect of the plant extract (100–330 mg/kg) was about 24–30% and is the result of the effect of a high concentration of ethanol. In the formalin test, the plant extract (41.3–330 mg/kg) significantly and dose-dependently inhibited nociception in both phases of the test with similar maximal effects of about 76% and 85%. Only the plant extract at the dose of 330 mg/kg caused a significant time-dependent reduction in time spent on the rotarod. CONCLUSIONS: In rats, the preventive systemic administration of the hydroethanolic extract of J. nigra leaf reduced chemically but not thermally induced pain. Higher efficacy was obtained in pain associated with inflammation and tissue injury. The antinociceptive effect is dose-dependent and may be limited by motor impairment. SAGE Publications 2022-08-17 /pmc/articles/PMC9393677/ /pubmed/36003322 http://dx.doi.org/10.1177/15593258221119877 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Inflammatory Parameters as Early Biomarkers in Prediction and Monitoring of Therapeutic Effects in Preclinical and Clinical Studies Srebro, Dragana Rajković, Katarina Dožić, Branko Vujović, Katarina Savić Brkić, Branislava Medić Milić, Petar Vučković, Sonja Investigation of the Antinociceptive Activity of the Hydroethanolic Extract of Junglas nigra Leaf by the Tail-Immersion and Formalin Pain Tests in Rats |
title | Investigation of the Antinociceptive Activity of the Hydroethanolic
Extract of Junglas nigra Leaf by the Tail-Immersion and
Formalin Pain Tests in Rats |
title_full | Investigation of the Antinociceptive Activity of the Hydroethanolic
Extract of Junglas nigra Leaf by the Tail-Immersion and
Formalin Pain Tests in Rats |
title_fullStr | Investigation of the Antinociceptive Activity of the Hydroethanolic
Extract of Junglas nigra Leaf by the Tail-Immersion and
Formalin Pain Tests in Rats |
title_full_unstemmed | Investigation of the Antinociceptive Activity of the Hydroethanolic
Extract of Junglas nigra Leaf by the Tail-Immersion and
Formalin Pain Tests in Rats |
title_short | Investigation of the Antinociceptive Activity of the Hydroethanolic
Extract of Junglas nigra Leaf by the Tail-Immersion and
Formalin Pain Tests in Rats |
title_sort | investigation of the antinociceptive activity of the hydroethanolic
extract of junglas nigra leaf by the tail-immersion and
formalin pain tests in rats |
topic | Inflammatory Parameters as Early Biomarkers in Prediction and Monitoring of Therapeutic Effects in Preclinical and Clinical Studies |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393677/ https://www.ncbi.nlm.nih.gov/pubmed/36003322 http://dx.doi.org/10.1177/15593258221119877 |
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