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Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells

BACKGROUND AND AIM: The oviduct environment is of particular importance because it is the site of fertilization and early embryo development. The oviduct, as a component of the reproductive system, responds to ovarian hormone (estradiol [E2] and progesterone [P4]) stimuli depending on the estrous cy...

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Autores principales: Lopera-Vásquez, Ricaurte, Uribe-García, Fabián, Rondón-Barragán, Iang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394134/
https://www.ncbi.nlm.nih.gov/pubmed/36185535
http://dx.doi.org/10.14202/vetworld.2022.1665-1675
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author Lopera-Vásquez, Ricaurte
Uribe-García, Fabián
Rondón-Barragán, Iang
author_facet Lopera-Vásquez, Ricaurte
Uribe-García, Fabián
Rondón-Barragán, Iang
author_sort Lopera-Vásquez, Ricaurte
collection PubMed
description BACKGROUND AND AIM: The oviduct environment is of particular importance because it is the site of fertilization and early embryo development. The oviduct, as a component of the reproductive system, responds to ovarian hormone (estradiol [E2] and progesterone [P4]) stimuli depending on the estrous cycle phase. This study aimed to elucidate the effect of estrous cycle phases (follicular and early and late luteal phases) on gene expression patterns in bovine oviduct epithelial cells (BOECs). MATERIALS AND METHODS: Oviducts were obtained from healthy slaughterhouse animals, corresponding to ipsilateral ovaries with dominant follicles or corpus luteum during early and late luteal phases. BOECs were recovered from the isthmus (IST) and ampulla (AMP), and the expression patterns of genes related to cytokinesis and mitosis mechanisms (rho-associated coiled-coil containing protein kinase and cellular communication network factor 2 [CCN2]), growth factors (insulin-like growth factor-binding protein 3, epidermal growth factor receptor [EGFR], vascular endothelial growth factor A, and EGFR), antioxidant mechanisms (glutathione peroxidase 4 [GPX4]), apoptosis (B-cell lymphoma 2), complement component (C3), energy metabolism (aldose reductase gene family 1-member b1 [AKRIB1] and solute carrier family 2), hormone receptors (estrogen receptor 1 and luteinizing hormone/choriogonadotropin receptor), and specific glycoproteins (oviductal glycoprotein 1) were analyzed. RESULTS: High P4 levels (late luteal phase) affected the expression of important genes related to antioxidant mechanisms (GPX4), energy metabolism (AKRIB1), growth factors (IGBP3 and EGFR), and cell growth regulation (CCN2) in the AMP. Low P4 levels (early luteal phase) affected the expression of AKR1B1, IGBP3, and CCN2. In addition, estrogen likely had an effect on OVPGP expression in the cattle oviduct. CONCLUSION: Differential gene expression patterns of BOECs in the AMP during the luteal phase (antioxidant mechanisms, energy metabolism, growth factors, and immunological regulators) and in the IST during the follicular phase (glycoproteins) may influence their renewal and population proportions, modulating the oviduct environment as well as gamete and embryo physiology.
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spelling pubmed-93941342022-09-30 Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells Lopera-Vásquez, Ricaurte Uribe-García, Fabián Rondón-Barragán, Iang Vet World Research Article BACKGROUND AND AIM: The oviduct environment is of particular importance because it is the site of fertilization and early embryo development. The oviduct, as a component of the reproductive system, responds to ovarian hormone (estradiol [E2] and progesterone [P4]) stimuli depending on the estrous cycle phase. This study aimed to elucidate the effect of estrous cycle phases (follicular and early and late luteal phases) on gene expression patterns in bovine oviduct epithelial cells (BOECs). MATERIALS AND METHODS: Oviducts were obtained from healthy slaughterhouse animals, corresponding to ipsilateral ovaries with dominant follicles or corpus luteum during early and late luteal phases. BOECs were recovered from the isthmus (IST) and ampulla (AMP), and the expression patterns of genes related to cytokinesis and mitosis mechanisms (rho-associated coiled-coil containing protein kinase and cellular communication network factor 2 [CCN2]), growth factors (insulin-like growth factor-binding protein 3, epidermal growth factor receptor [EGFR], vascular endothelial growth factor A, and EGFR), antioxidant mechanisms (glutathione peroxidase 4 [GPX4]), apoptosis (B-cell lymphoma 2), complement component (C3), energy metabolism (aldose reductase gene family 1-member b1 [AKRIB1] and solute carrier family 2), hormone receptors (estrogen receptor 1 and luteinizing hormone/choriogonadotropin receptor), and specific glycoproteins (oviductal glycoprotein 1) were analyzed. RESULTS: High P4 levels (late luteal phase) affected the expression of important genes related to antioxidant mechanisms (GPX4), energy metabolism (AKRIB1), growth factors (IGBP3 and EGFR), and cell growth regulation (CCN2) in the AMP. Low P4 levels (early luteal phase) affected the expression of AKR1B1, IGBP3, and CCN2. In addition, estrogen likely had an effect on OVPGP expression in the cattle oviduct. CONCLUSION: Differential gene expression patterns of BOECs in the AMP during the luteal phase (antioxidant mechanisms, energy metabolism, growth factors, and immunological regulators) and in the IST during the follicular phase (glycoproteins) may influence their renewal and population proportions, modulating the oviduct environment as well as gamete and embryo physiology. Veterinary World 2022-07 2022-07-14 /pmc/articles/PMC9394134/ /pubmed/36185535 http://dx.doi.org/10.14202/vetworld.2022.1665-1675 Text en Copyright: © Lopera-Vásquez, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Lopera-Vásquez, Ricaurte
Uribe-García, Fabián
Rondón-Barragán, Iang
Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells
title Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells
title_full Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells
title_fullStr Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells
title_full_unstemmed Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells
title_short Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells
title_sort effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394134/
https://www.ncbi.nlm.nih.gov/pubmed/36185535
http://dx.doi.org/10.14202/vetworld.2022.1665-1675
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