Cargando…

Lef1 and Dlx3 May Facilitate the Maturation of Secondary Hair Follicles in the Skin of Gansu Alpine Merino

Lymphatic enhancer factor 1 (Lef1) and distal-less homeobox 3 (Dlx3) are the transcription factors involved in regulating hair follicle development in mice, goats, and other animals. Their deletion can lead to hair follicle deficiency. In this study, hematoxylin–eosin staining (HE), real-time quanti...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Hongxian, He, Zhaohua, Xi, Qiming, Zhao, Fangfang, Hu, Jiang, Wang, Jiqing, Liu, Xiu, Zhao, Zhidong, Li, Mingna, Luo, Yuzhu, Li, Shaobin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394301/
https://www.ncbi.nlm.nih.gov/pubmed/35893063
http://dx.doi.org/10.3390/genes13081326
_version_ 1784771459114074112
author Sun, Hongxian
He, Zhaohua
Xi, Qiming
Zhao, Fangfang
Hu, Jiang
Wang, Jiqing
Liu, Xiu
Zhao, Zhidong
Li, Mingna
Luo, Yuzhu
Li, Shaobin
author_facet Sun, Hongxian
He, Zhaohua
Xi, Qiming
Zhao, Fangfang
Hu, Jiang
Wang, Jiqing
Liu, Xiu
Zhao, Zhidong
Li, Mingna
Luo, Yuzhu
Li, Shaobin
author_sort Sun, Hongxian
collection PubMed
description Lymphatic enhancer factor 1 (Lef1) and distal-less homeobox 3 (Dlx3) are the transcription factors involved in regulating hair follicle development in mice, goats, and other animals. Their deletion can lead to hair follicle deficiency. In this study, hematoxylin–eosin staining (HE), real-time quantitative PCR (RT-qPCR), immunohistochemistry, and immunofluorescence were used to analyze the expression, location, and biological functions of Lef1 and Dlx3 in the lateral skin of Gansu Alpine Merino aged 1, 30, 60, and 90 days. The results revealed that the number of hair follicles decreased with age and was significantly higher at 1 day than in the other three age groups (p < 0.05). The mRNA levels of Lef1 and Dlx3 in the skin of 30-day old Gansu Alpine Merino were significantly higher than those in the other three age groups (p < 0.05). Protein expression of Lef1 and Dlx3 was lowest at 1 day (p < 0.05) and peaked at 60 days. Lef1 and Dlx3 exhibited a high density and strong positive expression in the dermal papillae; additionally, Dlx3 exhibited a high density and strong positive expression in the inner and outer root sheaths. Collectively, Lef1 and Dlx3 may facilitate the maturation of secondary hair follicles, which is mainly achieved through the dermal papillae and inner and outer root sheaths.
format Online
Article
Text
id pubmed-9394301
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93943012022-08-23 Lef1 and Dlx3 May Facilitate the Maturation of Secondary Hair Follicles in the Skin of Gansu Alpine Merino Sun, Hongxian He, Zhaohua Xi, Qiming Zhao, Fangfang Hu, Jiang Wang, Jiqing Liu, Xiu Zhao, Zhidong Li, Mingna Luo, Yuzhu Li, Shaobin Genes (Basel) Article Lymphatic enhancer factor 1 (Lef1) and distal-less homeobox 3 (Dlx3) are the transcription factors involved in regulating hair follicle development in mice, goats, and other animals. Their deletion can lead to hair follicle deficiency. In this study, hematoxylin–eosin staining (HE), real-time quantitative PCR (RT-qPCR), immunohistochemistry, and immunofluorescence were used to analyze the expression, location, and biological functions of Lef1 and Dlx3 in the lateral skin of Gansu Alpine Merino aged 1, 30, 60, and 90 days. The results revealed that the number of hair follicles decreased with age and was significantly higher at 1 day than in the other three age groups (p < 0.05). The mRNA levels of Lef1 and Dlx3 in the skin of 30-day old Gansu Alpine Merino were significantly higher than those in the other three age groups (p < 0.05). Protein expression of Lef1 and Dlx3 was lowest at 1 day (p < 0.05) and peaked at 60 days. Lef1 and Dlx3 exhibited a high density and strong positive expression in the dermal papillae; additionally, Dlx3 exhibited a high density and strong positive expression in the inner and outer root sheaths. Collectively, Lef1 and Dlx3 may facilitate the maturation of secondary hair follicles, which is mainly achieved through the dermal papillae and inner and outer root sheaths. MDPI 2022-07-25 /pmc/articles/PMC9394301/ /pubmed/35893063 http://dx.doi.org/10.3390/genes13081326 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Hongxian
He, Zhaohua
Xi, Qiming
Zhao, Fangfang
Hu, Jiang
Wang, Jiqing
Liu, Xiu
Zhao, Zhidong
Li, Mingna
Luo, Yuzhu
Li, Shaobin
Lef1 and Dlx3 May Facilitate the Maturation of Secondary Hair Follicles in the Skin of Gansu Alpine Merino
title Lef1 and Dlx3 May Facilitate the Maturation of Secondary Hair Follicles in the Skin of Gansu Alpine Merino
title_full Lef1 and Dlx3 May Facilitate the Maturation of Secondary Hair Follicles in the Skin of Gansu Alpine Merino
title_fullStr Lef1 and Dlx3 May Facilitate the Maturation of Secondary Hair Follicles in the Skin of Gansu Alpine Merino
title_full_unstemmed Lef1 and Dlx3 May Facilitate the Maturation of Secondary Hair Follicles in the Skin of Gansu Alpine Merino
title_short Lef1 and Dlx3 May Facilitate the Maturation of Secondary Hair Follicles in the Skin of Gansu Alpine Merino
title_sort lef1 and dlx3 may facilitate the maturation of secondary hair follicles in the skin of gansu alpine merino
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394301/
https://www.ncbi.nlm.nih.gov/pubmed/35893063
http://dx.doi.org/10.3390/genes13081326
work_keys_str_mv AT sunhongxian lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino
AT hezhaohua lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino
AT xiqiming lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino
AT zhaofangfang lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino
AT hujiang lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino
AT wangjiqing lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino
AT liuxiu lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino
AT zhaozhidong lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino
AT limingna lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino
AT luoyuzhu lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino
AT lishaobin lef1anddlx3mayfacilitatethematurationofsecondaryhairfolliclesintheskinofgansualpinemerino