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Human Bocavirus infection among children with respiratory tract infection in Ibadan, Nigeria
Background. Human Bocavirus (HBoV), which is an ssDNA virus of the family Parvoviridae, is responsible for 21.5 % of childhood respiratory tract infections (RTIs) annually. Among the four genotypes currently known, HBoV-1 has been associated with acute RTI. Although there have been studies on HBoV i...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394526/ https://www.ncbi.nlm.nih.gov/pubmed/36003362 http://dx.doi.org/10.1099/acmi.0.000356 |
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author | Joseph, Olajumoke Olufunmilayo Adeniji, Johnson Adekunle Faneye, Adedayo Omotayo |
author_facet | Joseph, Olajumoke Olufunmilayo Adeniji, Johnson Adekunle Faneye, Adedayo Omotayo |
author_sort | Joseph, Olajumoke Olufunmilayo |
collection | PubMed |
description | Background. Human Bocavirus (HBoV), which is an ssDNA virus of the family Parvoviridae, is responsible for 21.5 % of childhood respiratory tract infections (RTIs) annually. Among the four genotypes currently known, HBoV-1 has been associated with acute RTI. Although there have been studies on HBoV in some countries, there is limited information on this virus in sub-Saharan Africa where there is the highest burden of RTI. This study aimed to characterize the circulating strains of HBoV in Ibadan, Nigeria. Methods. Nasopharyngeal and oropharyngeal swab samples were collected from 333 children ≤5 years old presenting with RTI attending hospitals in Ibadan, whose parents assented, from 2014 to 2015. Twenty-three HBoV isolates were sequenced after a nested PCR and phylogenetic analysis was carried out using mega 6 software. RESULTS. A total of 27 children tested positive for the HBoV-1 genotype by PCR and 23 of the 27 isolates were successfully sequenced. The 23 HBoV-1 isolates from this study have been assigned GenBank accession numbers KY701984–KY702006. Phylogram analysis indicated that the isolates belong to the same clades. Six isolates aligned closely to the reference strains ST1 and ST2, while 17 isolates showed a high level of divergence to the reference isolates. CONCLUSION. This study highlights the contribution of HBoV to RTIs in Nigeria and that HBoV-1 strains are associated with the infection. |
format | Online Article Text |
id | pubmed-9394526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-93945262022-08-23 Human Bocavirus infection among children with respiratory tract infection in Ibadan, Nigeria Joseph, Olajumoke Olufunmilayo Adeniji, Johnson Adekunle Faneye, Adedayo Omotayo Access Microbiol Research Articles Background. Human Bocavirus (HBoV), which is an ssDNA virus of the family Parvoviridae, is responsible for 21.5 % of childhood respiratory tract infections (RTIs) annually. Among the four genotypes currently known, HBoV-1 has been associated with acute RTI. Although there have been studies on HBoV in some countries, there is limited information on this virus in sub-Saharan Africa where there is the highest burden of RTI. This study aimed to characterize the circulating strains of HBoV in Ibadan, Nigeria. Methods. Nasopharyngeal and oropharyngeal swab samples were collected from 333 children ≤5 years old presenting with RTI attending hospitals in Ibadan, whose parents assented, from 2014 to 2015. Twenty-three HBoV isolates were sequenced after a nested PCR and phylogenetic analysis was carried out using mega 6 software. RESULTS. A total of 27 children tested positive for the HBoV-1 genotype by PCR and 23 of the 27 isolates were successfully sequenced. The 23 HBoV-1 isolates from this study have been assigned GenBank accession numbers KY701984–KY702006. Phylogram analysis indicated that the isolates belong to the same clades. Six isolates aligned closely to the reference strains ST1 and ST2, while 17 isolates showed a high level of divergence to the reference isolates. CONCLUSION. This study highlights the contribution of HBoV to RTIs in Nigeria and that HBoV-1 strains are associated with the infection. Microbiology Society 2022-05-23 /pmc/articles/PMC9394526/ /pubmed/36003362 http://dx.doi.org/10.1099/acmi.0.000356 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
spellingShingle | Research Articles Joseph, Olajumoke Olufunmilayo Adeniji, Johnson Adekunle Faneye, Adedayo Omotayo Human Bocavirus infection among children with respiratory tract infection in Ibadan, Nigeria |
title | Human Bocavirus infection among children with respiratory tract infection in Ibadan, Nigeria |
title_full | Human Bocavirus infection among children with respiratory tract infection in Ibadan, Nigeria |
title_fullStr | Human Bocavirus infection among children with respiratory tract infection in Ibadan, Nigeria |
title_full_unstemmed | Human Bocavirus infection among children with respiratory tract infection in Ibadan, Nigeria |
title_short | Human Bocavirus infection among children with respiratory tract infection in Ibadan, Nigeria |
title_sort | human bocavirus infection among children with respiratory tract infection in ibadan, nigeria |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394526/ https://www.ncbi.nlm.nih.gov/pubmed/36003362 http://dx.doi.org/10.1099/acmi.0.000356 |
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