Cargando…

Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways

Allyl methyl trisulfide (AMTS) is one major lipid-soluble organosulfur compound of garlic. Previous studies have reported the potential therapeutic effect of garlic on acute lung injury (ALI) or its severe condition acute respiratory distress syndrome (ARDS), but the specific substances that exert t...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Shuo, Liu, Jinqian, Dong, Jing, Fan, Zongqiang, Wang, Fugui, Wu, Ping, Li, Xiaojing, Kou, Ruirui, Chen, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394683/
https://www.ncbi.nlm.nih.gov/pubmed/36003507
http://dx.doi.org/10.3389/fphar.2022.919898
_version_ 1784771532709429248
author Wang, Shuo
Liu, Jinqian
Dong, Jing
Fan, Zongqiang
Wang, Fugui
Wu, Ping
Li, Xiaojing
Kou, Ruirui
Chen, Fang
author_facet Wang, Shuo
Liu, Jinqian
Dong, Jing
Fan, Zongqiang
Wang, Fugui
Wu, Ping
Li, Xiaojing
Kou, Ruirui
Chen, Fang
author_sort Wang, Shuo
collection PubMed
description Allyl methyl trisulfide (AMTS) is one major lipid-soluble organosulfur compound of garlic. Previous studies have reported the potential therapeutic effect of garlic on acute lung injury (ALI) or its severe condition acute respiratory distress syndrome (ARDS), but the specific substances that exert the regulatory effects are still unclear. In this study, we investigate the protective effects of AMTS on lipopolysaccharide (LPS)-induced ALI mice and explored the underlying mechanisms. In vivo experiments, ICR mice were pretreated with 25–100 mg/kg AMTS for 7 days and followed by intratracheal instillation of LPS (1.5 mg/kg). The results showed that AMTS significantly attenuated LPS-induced deterioration of lung pathology, demonstrated by ameliorative edema and protein leakage, and improved pulmonary histopathological morphology. Meanwhile, the expression of inflammatory mediators and the infiltration of inflammation-regulation cells induced by LPS were also inhibited. In vitro experiments also revealed that AMTS could alleviate inflammation response and inhibit the exaggeration of macrophage M1 polarization in LPS-induced RAW264.7 cells. Mechanistically, we identified that AMTS treatment could attenuate the LPS-induced elevation of protein expression of p-IκBα, nuclear NF-κB-p65, COX2, iNOS, p-P38, p-ERK1/2, and p-JNK. Collectively, these data suggest that AMTS could attenuate LPS-induced ALI and the molecular mechanisms should be related to the suppression of the NF-κB and MAPKs pathways.
format Online
Article
Text
id pubmed-9394683
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93946832022-08-23 Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways Wang, Shuo Liu, Jinqian Dong, Jing Fan, Zongqiang Wang, Fugui Wu, Ping Li, Xiaojing Kou, Ruirui Chen, Fang Front Pharmacol Pharmacology Allyl methyl trisulfide (AMTS) is one major lipid-soluble organosulfur compound of garlic. Previous studies have reported the potential therapeutic effect of garlic on acute lung injury (ALI) or its severe condition acute respiratory distress syndrome (ARDS), but the specific substances that exert the regulatory effects are still unclear. In this study, we investigate the protective effects of AMTS on lipopolysaccharide (LPS)-induced ALI mice and explored the underlying mechanisms. In vivo experiments, ICR mice were pretreated with 25–100 mg/kg AMTS for 7 days and followed by intratracheal instillation of LPS (1.5 mg/kg). The results showed that AMTS significantly attenuated LPS-induced deterioration of lung pathology, demonstrated by ameliorative edema and protein leakage, and improved pulmonary histopathological morphology. Meanwhile, the expression of inflammatory mediators and the infiltration of inflammation-regulation cells induced by LPS were also inhibited. In vitro experiments also revealed that AMTS could alleviate inflammation response and inhibit the exaggeration of macrophage M1 polarization in LPS-induced RAW264.7 cells. Mechanistically, we identified that AMTS treatment could attenuate the LPS-induced elevation of protein expression of p-IκBα, nuclear NF-κB-p65, COX2, iNOS, p-P38, p-ERK1/2, and p-JNK. Collectively, these data suggest that AMTS could attenuate LPS-induced ALI and the molecular mechanisms should be related to the suppression of the NF-κB and MAPKs pathways. Frontiers Media S.A. 2022-08-08 /pmc/articles/PMC9394683/ /pubmed/36003507 http://dx.doi.org/10.3389/fphar.2022.919898 Text en Copyright © 2022 Wang, Liu, Dong, Fan, Wang, Wu, Li, Kou and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Wang, Shuo
Liu, Jinqian
Dong, Jing
Fan, Zongqiang
Wang, Fugui
Wu, Ping
Li, Xiaojing
Kou, Ruirui
Chen, Fang
Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways
title Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways
title_full Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways
title_fullStr Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways
title_full_unstemmed Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways
title_short Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways
title_sort allyl methyl trisulfide protected against lps-induced acute lung injury in mice via inhibition of the nf-κb and mapk pathways
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394683/
https://www.ncbi.nlm.nih.gov/pubmed/36003507
http://dx.doi.org/10.3389/fphar.2022.919898
work_keys_str_mv AT wangshuo allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways
AT liujinqian allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways
AT dongjing allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways
AT fanzongqiang allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways
AT wangfugui allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways
AT wuping allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways
AT lixiaojing allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways
AT kouruirui allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways
AT chenfang allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways