Cargando…
Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways
Allyl methyl trisulfide (AMTS) is one major lipid-soluble organosulfur compound of garlic. Previous studies have reported the potential therapeutic effect of garlic on acute lung injury (ALI) or its severe condition acute respiratory distress syndrome (ARDS), but the specific substances that exert t...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394683/ https://www.ncbi.nlm.nih.gov/pubmed/36003507 http://dx.doi.org/10.3389/fphar.2022.919898 |
_version_ | 1784771532709429248 |
---|---|
author | Wang, Shuo Liu, Jinqian Dong, Jing Fan, Zongqiang Wang, Fugui Wu, Ping Li, Xiaojing Kou, Ruirui Chen, Fang |
author_facet | Wang, Shuo Liu, Jinqian Dong, Jing Fan, Zongqiang Wang, Fugui Wu, Ping Li, Xiaojing Kou, Ruirui Chen, Fang |
author_sort | Wang, Shuo |
collection | PubMed |
description | Allyl methyl trisulfide (AMTS) is one major lipid-soluble organosulfur compound of garlic. Previous studies have reported the potential therapeutic effect of garlic on acute lung injury (ALI) or its severe condition acute respiratory distress syndrome (ARDS), but the specific substances that exert the regulatory effects are still unclear. In this study, we investigate the protective effects of AMTS on lipopolysaccharide (LPS)-induced ALI mice and explored the underlying mechanisms. In vivo experiments, ICR mice were pretreated with 25–100 mg/kg AMTS for 7 days and followed by intratracheal instillation of LPS (1.5 mg/kg). The results showed that AMTS significantly attenuated LPS-induced deterioration of lung pathology, demonstrated by ameliorative edema and protein leakage, and improved pulmonary histopathological morphology. Meanwhile, the expression of inflammatory mediators and the infiltration of inflammation-regulation cells induced by LPS were also inhibited. In vitro experiments also revealed that AMTS could alleviate inflammation response and inhibit the exaggeration of macrophage M1 polarization in LPS-induced RAW264.7 cells. Mechanistically, we identified that AMTS treatment could attenuate the LPS-induced elevation of protein expression of p-IκBα, nuclear NF-κB-p65, COX2, iNOS, p-P38, p-ERK1/2, and p-JNK. Collectively, these data suggest that AMTS could attenuate LPS-induced ALI and the molecular mechanisms should be related to the suppression of the NF-κB and MAPKs pathways. |
format | Online Article Text |
id | pubmed-9394683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93946832022-08-23 Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways Wang, Shuo Liu, Jinqian Dong, Jing Fan, Zongqiang Wang, Fugui Wu, Ping Li, Xiaojing Kou, Ruirui Chen, Fang Front Pharmacol Pharmacology Allyl methyl trisulfide (AMTS) is one major lipid-soluble organosulfur compound of garlic. Previous studies have reported the potential therapeutic effect of garlic on acute lung injury (ALI) or its severe condition acute respiratory distress syndrome (ARDS), but the specific substances that exert the regulatory effects are still unclear. In this study, we investigate the protective effects of AMTS on lipopolysaccharide (LPS)-induced ALI mice and explored the underlying mechanisms. In vivo experiments, ICR mice were pretreated with 25–100 mg/kg AMTS for 7 days and followed by intratracheal instillation of LPS (1.5 mg/kg). The results showed that AMTS significantly attenuated LPS-induced deterioration of lung pathology, demonstrated by ameliorative edema and protein leakage, and improved pulmonary histopathological morphology. Meanwhile, the expression of inflammatory mediators and the infiltration of inflammation-regulation cells induced by LPS were also inhibited. In vitro experiments also revealed that AMTS could alleviate inflammation response and inhibit the exaggeration of macrophage M1 polarization in LPS-induced RAW264.7 cells. Mechanistically, we identified that AMTS treatment could attenuate the LPS-induced elevation of protein expression of p-IκBα, nuclear NF-κB-p65, COX2, iNOS, p-P38, p-ERK1/2, and p-JNK. Collectively, these data suggest that AMTS could attenuate LPS-induced ALI and the molecular mechanisms should be related to the suppression of the NF-κB and MAPKs pathways. Frontiers Media S.A. 2022-08-08 /pmc/articles/PMC9394683/ /pubmed/36003507 http://dx.doi.org/10.3389/fphar.2022.919898 Text en Copyright © 2022 Wang, Liu, Dong, Fan, Wang, Wu, Li, Kou and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Wang, Shuo Liu, Jinqian Dong, Jing Fan, Zongqiang Wang, Fugui Wu, Ping Li, Xiaojing Kou, Ruirui Chen, Fang Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways |
title | Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways |
title_full | Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways |
title_fullStr | Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways |
title_full_unstemmed | Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways |
title_short | Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways |
title_sort | allyl methyl trisulfide protected against lps-induced acute lung injury in mice via inhibition of the nf-κb and mapk pathways |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394683/ https://www.ncbi.nlm.nih.gov/pubmed/36003507 http://dx.doi.org/10.3389/fphar.2022.919898 |
work_keys_str_mv | AT wangshuo allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways AT liujinqian allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways AT dongjing allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways AT fanzongqiang allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways AT wangfugui allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways AT wuping allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways AT lixiaojing allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways AT kouruirui allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways AT chenfang allylmethyltrisulfideprotectedagainstlpsinducedacutelunginjuryinmiceviainhibitionofthenfkbandmapkpathways |