Cargando…

Heat acclimation ameliorated heat stress-induced acute kidney injury and prevented changes in kidney macrophages and fibrosis

Heatstroke can cause acute kidney injury (AKI), which reportedly progresses to chronic kidney disease. Kidney macrophages may be involved in such injury. Although heat acclimation (HA) provides thermal resilience, its renoprotective effect and mechanism remain unclear. To investigate heat stress-ind...

Descripción completa

Detalles Bibliográficos
Autores principales: Goto, Hiroyasu, Nakashima, Masahiro, Nakashima, Hiroyuki, Noguchi, Midori, Imakiire, Toshihiko, Oshima, Naoki, Kinoshita, Manabu, Kumagai, Hiroo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Physiological Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394728/
https://www.ncbi.nlm.nih.gov/pubmed/35796461
http://dx.doi.org/10.1152/ajprenal.00065.2022
_version_ 1784771541961015296
author Goto, Hiroyasu
Nakashima, Masahiro
Nakashima, Hiroyuki
Noguchi, Midori
Imakiire, Toshihiko
Oshima, Naoki
Kinoshita, Manabu
Kumagai, Hiroo
author_facet Goto, Hiroyasu
Nakashima, Masahiro
Nakashima, Hiroyuki
Noguchi, Midori
Imakiire, Toshihiko
Oshima, Naoki
Kinoshita, Manabu
Kumagai, Hiroo
author_sort Goto, Hiroyasu
collection PubMed
description Heatstroke can cause acute kidney injury (AKI), which reportedly progresses to chronic kidney disease. Kidney macrophages may be involved in such injury. Although heat acclimation (HA) provides thermal resilience, its renoprotective effect and mechanism remain unclear. To investigate heat stress-induced kidney injuries in mice and the mitigating effect of HA on them, male C57/BL6J mice were exposed to heat stress (40°C, 1 h) with or without 5-day HA (38°C, 3 h/day) prior to heat stress. Heat stress damaged kidney proximal tubules with an elevation of urinary kidney injury molecule-1. Kidney fibrosis was observed on day 7 and correlated with urinary kidney injury molecule-1 levels on day 3. Kidney resident macrophages decreased on day 1, whereas the number of infiltrating macrophages in the kidney did not change. Both subsets of macrophages polarized to the proinflammatory M1 phenotype on day 1; however, they polarized to the anti-inflammatory M2 phenotype on day 7. HA significantly ameliorated heat stress-induced proximal tubular damage and kidney fibrosis. HA substantially increased heat shock protein 70 expression in the tubules before heat stress and reduced the elevation of cleaved caspase-3 expression after heat stress. HA also induced heat shock protein 70 expression of resident macrophages and prevented heat stress-induced changes in both subsets of kidney macrophages. These results provide pathophysiological data supporting the renoprotective effect of HA. Further studies are needed to confirm that HA can prevent kidney damage due to heat stress in humans. NEW & NOTEWORTHY Heat stress could induce acute kidney injury. Although heat acclimation (HA) reportedly provides thermal tolerance, its effect on heat stress-induced kidney damage remains unclear. This study showed that 5-day HA ameliorates mouse kidney tubular damage and subsequent fibrosis caused by heat stress. It also demonstrated that HA enhances intracellular heat shock protein 70 expression in tubular cells and prevents a decrease in kidney resident macrophages, which explains the renoprotective effect of HA.
format Online
Article
Text
id pubmed-9394728
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Physiological Society
record_format MEDLINE/PubMed
spelling pubmed-93947282022-08-31 Heat acclimation ameliorated heat stress-induced acute kidney injury and prevented changes in kidney macrophages and fibrosis Goto, Hiroyasu Nakashima, Masahiro Nakashima, Hiroyuki Noguchi, Midori Imakiire, Toshihiko Oshima, Naoki Kinoshita, Manabu Kumagai, Hiroo Am J Physiol Renal Physiol Research Article Heatstroke can cause acute kidney injury (AKI), which reportedly progresses to chronic kidney disease. Kidney macrophages may be involved in such injury. Although heat acclimation (HA) provides thermal resilience, its renoprotective effect and mechanism remain unclear. To investigate heat stress-induced kidney injuries in mice and the mitigating effect of HA on them, male C57/BL6J mice were exposed to heat stress (40°C, 1 h) with or without 5-day HA (38°C, 3 h/day) prior to heat stress. Heat stress damaged kidney proximal tubules with an elevation of urinary kidney injury molecule-1. Kidney fibrosis was observed on day 7 and correlated with urinary kidney injury molecule-1 levels on day 3. Kidney resident macrophages decreased on day 1, whereas the number of infiltrating macrophages in the kidney did not change. Both subsets of macrophages polarized to the proinflammatory M1 phenotype on day 1; however, they polarized to the anti-inflammatory M2 phenotype on day 7. HA significantly ameliorated heat stress-induced proximal tubular damage and kidney fibrosis. HA substantially increased heat shock protein 70 expression in the tubules before heat stress and reduced the elevation of cleaved caspase-3 expression after heat stress. HA also induced heat shock protein 70 expression of resident macrophages and prevented heat stress-induced changes in both subsets of kidney macrophages. These results provide pathophysiological data supporting the renoprotective effect of HA. Further studies are needed to confirm that HA can prevent kidney damage due to heat stress in humans. NEW & NOTEWORTHY Heat stress could induce acute kidney injury. Although heat acclimation (HA) reportedly provides thermal tolerance, its effect on heat stress-induced kidney damage remains unclear. This study showed that 5-day HA ameliorates mouse kidney tubular damage and subsequent fibrosis caused by heat stress. It also demonstrated that HA enhances intracellular heat shock protein 70 expression in tubular cells and prevents a decrease in kidney resident macrophages, which explains the renoprotective effect of HA. American Physiological Society 2022-09-01 2022-07-07 /pmc/articles/PMC9394728/ /pubmed/35796461 http://dx.doi.org/10.1152/ajprenal.00065.2022 Text en Copyright © 2022 The Authors https://creativecommons.org/licenses/by/4.0/Licensed under Creative Commons Attribution CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/) . Published by the American Physiological Society.
spellingShingle Research Article
Goto, Hiroyasu
Nakashima, Masahiro
Nakashima, Hiroyuki
Noguchi, Midori
Imakiire, Toshihiko
Oshima, Naoki
Kinoshita, Manabu
Kumagai, Hiroo
Heat acclimation ameliorated heat stress-induced acute kidney injury and prevented changes in kidney macrophages and fibrosis
title Heat acclimation ameliorated heat stress-induced acute kidney injury and prevented changes in kidney macrophages and fibrosis
title_full Heat acclimation ameliorated heat stress-induced acute kidney injury and prevented changes in kidney macrophages and fibrosis
title_fullStr Heat acclimation ameliorated heat stress-induced acute kidney injury and prevented changes in kidney macrophages and fibrosis
title_full_unstemmed Heat acclimation ameliorated heat stress-induced acute kidney injury and prevented changes in kidney macrophages and fibrosis
title_short Heat acclimation ameliorated heat stress-induced acute kidney injury and prevented changes in kidney macrophages and fibrosis
title_sort heat acclimation ameliorated heat stress-induced acute kidney injury and prevented changes in kidney macrophages and fibrosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394728/
https://www.ncbi.nlm.nih.gov/pubmed/35796461
http://dx.doi.org/10.1152/ajprenal.00065.2022
work_keys_str_mv AT gotohiroyasu heatacclimationamelioratedheatstressinducedacutekidneyinjuryandpreventedchangesinkidneymacrophagesandfibrosis
AT nakashimamasahiro heatacclimationamelioratedheatstressinducedacutekidneyinjuryandpreventedchangesinkidneymacrophagesandfibrosis
AT nakashimahiroyuki heatacclimationamelioratedheatstressinducedacutekidneyinjuryandpreventedchangesinkidneymacrophagesandfibrosis
AT noguchimidori heatacclimationamelioratedheatstressinducedacutekidneyinjuryandpreventedchangesinkidneymacrophagesandfibrosis
AT imakiiretoshihiko heatacclimationamelioratedheatstressinducedacutekidneyinjuryandpreventedchangesinkidneymacrophagesandfibrosis
AT oshimanaoki heatacclimationamelioratedheatstressinducedacutekidneyinjuryandpreventedchangesinkidneymacrophagesandfibrosis
AT kinoshitamanabu heatacclimationamelioratedheatstressinducedacutekidneyinjuryandpreventedchangesinkidneymacrophagesandfibrosis
AT kumagaihiroo heatacclimationamelioratedheatstressinducedacutekidneyinjuryandpreventedchangesinkidneymacrophagesandfibrosis