mCherry contains a fluorescent protein isoform that interferes with its reporter function

Fluorescent proteins are essential reporters in cell and molecular biology. Here, we found that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform in both prokaryotes and eukaryotes. The short isoform creates significant backg...

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Detalles Bibliográficos
Autores principales: Fages-Lartaud, Maxime, Tietze, Lisa, Elie, Florence, Lale, Rahmi, Hohmann-Marriott, Martin Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395592/
https://www.ncbi.nlm.nih.gov/pubmed/36017355
http://dx.doi.org/10.3389/fbioe.2022.892138

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395592/
https://www.ncbi.nlm.nih.gov/pubmed/36017355
http://dx.doi.org/10.3389/fbioe.2022.892138

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