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mCherry contains a fluorescent protein isoform that interferes with its reporter function

Fluorescent proteins are essential reporters in cell and molecular biology. Here, we found that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform in both prokaryotes and eukaryotes. The short isoform creates significant backg...

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Detalles Bibliográficos
Autores principales:	Fages-Lartaud, Maxime, Tietze, Lisa, Elie, Florence, Lale, Rahmi, Hohmann-Marriott, Martin Frank
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395592/ https://www.ncbi.nlm.nih.gov/pubmed/36017355 http://dx.doi.org/10.3389/fbioe.2022.892138

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