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In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration
BACKGROUND: Many patients lose their organs or tissues due to disease, trauma, or a variety of genetic disorders. Tissue engineering is a multidisciplinary science to regenerate or restore tissue or organ function and an appropriate scaffold is the first and certainly a crucial step in tissue engine...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shiraz University of Medical Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395625/ https://www.ncbi.nlm.nih.gov/pubmed/36059279 http://dx.doi.org/10.31661/jbpe.v0i0.1188 |
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author | Fekrazad, Reza Tondnevis, Farbod Abolhasani, Mohamad Mahdi |
author_facet | Fekrazad, Reza Tondnevis, Farbod Abolhasani, Mohamad Mahdi |
author_sort | Fekrazad, Reza |
collection | PubMed |
description | BACKGROUND: Many patients lose their organs or tissues due to disease, trauma, or a variety of genetic disorders. Tissue engineering is a multidisciplinary science to regenerate or restore tissue or organ function and an appropriate scaffold is the first and certainly a crucial step in tissue engineering strategies. OBJECTIVE: The purpose of this study is to fabricate and evaluate the in-vitro response of porous nano Polycaprolactone (PCL)/ chitosan/ multi-wall carbon nanotube (MWCNTs) scaffold for tissue regeneration. MATERIAL AND METHODS: In this experimental research, a novel scaffold containing MWCNTs in polycaprolactone/chitosan nanofibrous scaffold was synthesized by electrospinning technique. RESULTS: According to scanning electron microscopy SEM images, by increasing the number of MWCNT in the scaffold by 2%, the average diameter decreased significantly for fabricated scaffolds with 5% MWCNTs. Based on the results, the scaffolds plunged from submicron to nanoscale fibers at about 80 nm. In addition, by adding more MWCNT to the nanofibrous scaffold, the biodegradation rate was decreased by 32%. However, mechanical characterization demonstrates that the higher level of MWCNT increases young modulus by 96%, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay illustrated that MWCNTs could enhance bioactivity and cell- scaffold relationship in addition to alkaline phosphatase (ALP). CONCLUSION: MWCNT significantly improves the physical and mechanical properties of fabricated scaffolds and in-vitro assessment demonstrated that the prepared nanofibrous scaffold containing 4% MWCNT could be a very useful biocompatible material for tissue engineering. |
format | Online Article Text |
id | pubmed-9395625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Shiraz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-93956252022-09-02 In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration Fekrazad, Reza Tondnevis, Farbod Abolhasani, Mohamad Mahdi J Biomed Phys Eng Original Article BACKGROUND: Many patients lose their organs or tissues due to disease, trauma, or a variety of genetic disorders. Tissue engineering is a multidisciplinary science to regenerate or restore tissue or organ function and an appropriate scaffold is the first and certainly a crucial step in tissue engineering strategies. OBJECTIVE: The purpose of this study is to fabricate and evaluate the in-vitro response of porous nano Polycaprolactone (PCL)/ chitosan/ multi-wall carbon nanotube (MWCNTs) scaffold for tissue regeneration. MATERIAL AND METHODS: In this experimental research, a novel scaffold containing MWCNTs in polycaprolactone/chitosan nanofibrous scaffold was synthesized by electrospinning technique. RESULTS: According to scanning electron microscopy SEM images, by increasing the number of MWCNT in the scaffold by 2%, the average diameter decreased significantly for fabricated scaffolds with 5% MWCNTs. Based on the results, the scaffolds plunged from submicron to nanoscale fibers at about 80 nm. In addition, by adding more MWCNT to the nanofibrous scaffold, the biodegradation rate was decreased by 32%. However, mechanical characterization demonstrates that the higher level of MWCNT increases young modulus by 96%, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay illustrated that MWCNTs could enhance bioactivity and cell- scaffold relationship in addition to alkaline phosphatase (ALP). CONCLUSION: MWCNT significantly improves the physical and mechanical properties of fabricated scaffolds and in-vitro assessment demonstrated that the prepared nanofibrous scaffold containing 4% MWCNT could be a very useful biocompatible material for tissue engineering. Shiraz University of Medical Sciences 2022-08-01 /pmc/articles/PMC9395625/ /pubmed/36059279 http://dx.doi.org/10.31661/jbpe.v0i0.1188 Text en Copyright: © Journal of Biomedical Physics and Engineering https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 Unported License, ( http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Fekrazad, Reza Tondnevis, Farbod Abolhasani, Mohamad Mahdi In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
title | In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
title_full | In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
title_fullStr | In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
title_full_unstemmed | In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
title_short | In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
title_sort | in-vitro evaluation of novel polycaprolactone/ chitosan/ carbon nano tube scaffold for tissue regeneration |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395625/ https://www.ncbi.nlm.nih.gov/pubmed/36059279 http://dx.doi.org/10.31661/jbpe.v0i0.1188 |
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