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MiRNA and associated inflammatory changes from baseline to hypoglycemia in type 2 diabetes
OBJECTIVE: Hypoglycemia in type 2 diabetes (T2D) increases morbidity and mortality but the underlying physiological response is still not fully understood, though physiological changes are still apparent 24 hours after the event. Small noncoding microRNA (miRNA) have multiple downstream biological e...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395634/ https://www.ncbi.nlm.nih.gov/pubmed/36017315 http://dx.doi.org/10.3389/fendo.2022.917041 |
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author | Ramanjaneya, Manjunath Priyanka, Ruth Bensila, Milin Jerobin, Jayakumar Pawar, Krunal Sathyapalan, Thozhukat Abou-Samra, Abdul Badi Halabi, Najeeb M. Moin, Abu Saleh Md Atkin, Stephen L. Butler, Alexandra E. |
author_facet | Ramanjaneya, Manjunath Priyanka, Ruth Bensila, Milin Jerobin, Jayakumar Pawar, Krunal Sathyapalan, Thozhukat Abou-Samra, Abdul Badi Halabi, Najeeb M. Moin, Abu Saleh Md Atkin, Stephen L. Butler, Alexandra E. |
author_sort | Ramanjaneya, Manjunath |
collection | PubMed |
description | OBJECTIVE: Hypoglycemia in type 2 diabetes (T2D) increases morbidity and mortality but the underlying physiological response is still not fully understood, though physiological changes are still apparent 24 hours after the event. Small noncoding microRNA (miRNA) have multiple downstream biological effects that may respond rapidly to stress. We hypothesized that hypoglycemia would induce rapid miRNA changes; therefore, this pilot exploratory study was undertaken. METHODS: A pilot prospective, parallel study in T2D (n=23) and controls (n=23). Insulin-induced hypoglycemia (2mmol/l: 36mg/dl) was induced and blood sampling performed at baseline and hypoglycemia. Initial profiling of miRNA was undertaken on pooled samples identified 96 miRNA that were differentially regulated, followed by validation on a custom designed 112 miRNA panel. RESULTS: Nine miRNAs differed from baseline to hypoglycemia in control subjects; eight were upregulated: miR-1303, miR-let-7e-5p, miR-1267, miR-30a-5p, miR-571, miR-661, miR-770-5p, miR-892b and one was downregulated: miR-652-3p. None of the miRNAs differed from baseline in T2D subjects. CONCLUSION: A rapid miRNA response reflecting protective pathways was seen in control subjects that appeared to be lost in T2D, suggesting that mitigating responses to hypoglycemia with blunting of the counter-regulatory response in T2D occurs even in patients with short duration of disease. CLINICAL TRIAL REGISTRATION: https://clinicaltrials.gov/ct2/show/NCT03102801?term=NCT03102801&draw=2&rank=1, identifier NCT03102801. |
format | Online Article Text |
id | pubmed-9395634 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93956342022-08-24 MiRNA and associated inflammatory changes from baseline to hypoglycemia in type 2 diabetes Ramanjaneya, Manjunath Priyanka, Ruth Bensila, Milin Jerobin, Jayakumar Pawar, Krunal Sathyapalan, Thozhukat Abou-Samra, Abdul Badi Halabi, Najeeb M. Moin, Abu Saleh Md Atkin, Stephen L. Butler, Alexandra E. Front Endocrinol (Lausanne) Endocrinology OBJECTIVE: Hypoglycemia in type 2 diabetes (T2D) increases morbidity and mortality but the underlying physiological response is still not fully understood, though physiological changes are still apparent 24 hours after the event. Small noncoding microRNA (miRNA) have multiple downstream biological effects that may respond rapidly to stress. We hypothesized that hypoglycemia would induce rapid miRNA changes; therefore, this pilot exploratory study was undertaken. METHODS: A pilot prospective, parallel study in T2D (n=23) and controls (n=23). Insulin-induced hypoglycemia (2mmol/l: 36mg/dl) was induced and blood sampling performed at baseline and hypoglycemia. Initial profiling of miRNA was undertaken on pooled samples identified 96 miRNA that were differentially regulated, followed by validation on a custom designed 112 miRNA panel. RESULTS: Nine miRNAs differed from baseline to hypoglycemia in control subjects; eight were upregulated: miR-1303, miR-let-7e-5p, miR-1267, miR-30a-5p, miR-571, miR-661, miR-770-5p, miR-892b and one was downregulated: miR-652-3p. None of the miRNAs differed from baseline in T2D subjects. CONCLUSION: A rapid miRNA response reflecting protective pathways was seen in control subjects that appeared to be lost in T2D, suggesting that mitigating responses to hypoglycemia with blunting of the counter-regulatory response in T2D occurs even in patients with short duration of disease. CLINICAL TRIAL REGISTRATION: https://clinicaltrials.gov/ct2/show/NCT03102801?term=NCT03102801&draw=2&rank=1, identifier NCT03102801. Frontiers Media S.A. 2022-08-09 /pmc/articles/PMC9395634/ /pubmed/36017315 http://dx.doi.org/10.3389/fendo.2022.917041 Text en Copyright © 2022 Ramanjaneya, Priyanka, Bensila, Jerobin, Pawar, Sathyapalan, Abou-Samra, Halabi, Moin, Atkin and Butler https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Ramanjaneya, Manjunath Priyanka, Ruth Bensila, Milin Jerobin, Jayakumar Pawar, Krunal Sathyapalan, Thozhukat Abou-Samra, Abdul Badi Halabi, Najeeb M. Moin, Abu Saleh Md Atkin, Stephen L. Butler, Alexandra E. MiRNA and associated inflammatory changes from baseline to hypoglycemia in type 2 diabetes |
title | MiRNA and associated inflammatory changes from baseline to hypoglycemia in type 2 diabetes |
title_full | MiRNA and associated inflammatory changes from baseline to hypoglycemia in type 2 diabetes |
title_fullStr | MiRNA and associated inflammatory changes from baseline to hypoglycemia in type 2 diabetes |
title_full_unstemmed | MiRNA and associated inflammatory changes from baseline to hypoglycemia in type 2 diabetes |
title_short | MiRNA and associated inflammatory changes from baseline to hypoglycemia in type 2 diabetes |
title_sort | mirna and associated inflammatory changes from baseline to hypoglycemia in type 2 diabetes |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395634/ https://www.ncbi.nlm.nih.gov/pubmed/36017315 http://dx.doi.org/10.3389/fendo.2022.917041 |
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