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Promoter Methylation of QKI as a Potential Specific Biomarker for Early Detection of Colorectal Cancer
Early and specific detection of cancer provides an opportunity for appropriate treatment. Although studies have suggested that QKI is a tumor suppressor gene, no studies have evaluated the diagnostic utility of QKI methylation in colorectal cancer (CRC). Here, we evaluated the methylation status of...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395658/ https://www.ncbi.nlm.nih.gov/pubmed/36017498 http://dx.doi.org/10.3389/fgene.2022.928150 |
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author | Zhang, Lei Li, Dapeng Gao, Lijing Fu, Jinming Sun, Simin Huang, Hao Zhang, Ding Jia, Chenyang Zheng, Ting Cui, Binbin Liu, Yanlong Zhao, Yashuang |
author_facet | Zhang, Lei Li, Dapeng Gao, Lijing Fu, Jinming Sun, Simin Huang, Hao Zhang, Ding Jia, Chenyang Zheng, Ting Cui, Binbin Liu, Yanlong Zhao, Yashuang |
author_sort | Zhang, Lei |
collection | PubMed |
description | Early and specific detection of cancer provides an opportunity for appropriate treatment. Although studies have suggested that QKI is a tumor suppressor gene, no studies have evaluated the diagnostic utility of QKI methylation in colorectal cancer (CRC). Here, we evaluated the methylation status of QKI by integrating the methylation data of tissues and cell lines of multiple cancer types. The diagnostic performance of QKI was analyzed in the discovery dataset from the TCGA CRC 450K array (n = 440) and tested in the test sets (n = 845) from the GEO. The methylation level of QKI was further validated in our independent dataset (n = 388) using targeted bisulfite sequencing. All detected CpG sites in the QKI promoter showed CRC-specific hypermethylation in 31 types of tumor tissues. In the discovery dataset, six consecutive CpG sites achieved high diagnostic performances, with AUCs ranging from 0.821 to 0.930. In the test set, a region (chr6: 163,834,452–163,834,924) including four consecutive CpG sites had robust diagnostic ability in distinguishing CRC and adenoma from normal samples. In the validation dataset, similar robust results were observed in both early- and advanced-stage CRC patients. In addition, QKI exhibited hypermethylation in the cfDNA of patients with CRC (n = 14). Collectively, the QKI promoter is a CRC-specific methylation biomarker and holds great promise for improving the diagnosis using minimally invasive biopsy. |
format | Online Article Text |
id | pubmed-9395658 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93956582022-08-24 Promoter Methylation of QKI as a Potential Specific Biomarker for Early Detection of Colorectal Cancer Zhang, Lei Li, Dapeng Gao, Lijing Fu, Jinming Sun, Simin Huang, Hao Zhang, Ding Jia, Chenyang Zheng, Ting Cui, Binbin Liu, Yanlong Zhao, Yashuang Front Genet Genetics Early and specific detection of cancer provides an opportunity for appropriate treatment. Although studies have suggested that QKI is a tumor suppressor gene, no studies have evaluated the diagnostic utility of QKI methylation in colorectal cancer (CRC). Here, we evaluated the methylation status of QKI by integrating the methylation data of tissues and cell lines of multiple cancer types. The diagnostic performance of QKI was analyzed in the discovery dataset from the TCGA CRC 450K array (n = 440) and tested in the test sets (n = 845) from the GEO. The methylation level of QKI was further validated in our independent dataset (n = 388) using targeted bisulfite sequencing. All detected CpG sites in the QKI promoter showed CRC-specific hypermethylation in 31 types of tumor tissues. In the discovery dataset, six consecutive CpG sites achieved high diagnostic performances, with AUCs ranging from 0.821 to 0.930. In the test set, a region (chr6: 163,834,452–163,834,924) including four consecutive CpG sites had robust diagnostic ability in distinguishing CRC and adenoma from normal samples. In the validation dataset, similar robust results were observed in both early- and advanced-stage CRC patients. In addition, QKI exhibited hypermethylation in the cfDNA of patients with CRC (n = 14). Collectively, the QKI promoter is a CRC-specific methylation biomarker and holds great promise for improving the diagnosis using minimally invasive biopsy. Frontiers Media S.A. 2022-08-09 /pmc/articles/PMC9395658/ /pubmed/36017498 http://dx.doi.org/10.3389/fgene.2022.928150 Text en Copyright © 2022 Zhang, Li, Gao, Fu, Sun, Huang, Zhang, Jia, Zheng, Cui, Liu and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Zhang, Lei Li, Dapeng Gao, Lijing Fu, Jinming Sun, Simin Huang, Hao Zhang, Ding Jia, Chenyang Zheng, Ting Cui, Binbin Liu, Yanlong Zhao, Yashuang Promoter Methylation of QKI as a Potential Specific Biomarker for Early Detection of Colorectal Cancer |
title | Promoter Methylation of QKI as a Potential Specific Biomarker for Early Detection of Colorectal Cancer |
title_full | Promoter Methylation of QKI as a Potential Specific Biomarker for Early Detection of Colorectal Cancer |
title_fullStr | Promoter Methylation of QKI as a Potential Specific Biomarker for Early Detection of Colorectal Cancer |
title_full_unstemmed | Promoter Methylation of QKI as a Potential Specific Biomarker for Early Detection of Colorectal Cancer |
title_short | Promoter Methylation of QKI as a Potential Specific Biomarker for Early Detection of Colorectal Cancer |
title_sort | promoter methylation of qki as a potential specific biomarker for early detection of colorectal cancer |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395658/ https://www.ncbi.nlm.nih.gov/pubmed/36017498 http://dx.doi.org/10.3389/fgene.2022.928150 |
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