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Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery

Novel alginate-hydroxyapatite hybrid microspheres were developed for simultaneous delivery of drugs and cells as a multifunctional bone substitute for osteoporotic bone tissue regeneration. The microspheres were used to enhance osteogenesis and to carry and deliver quercetin, a representative phytoe...

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Autores principales: Kim, Jueun, Choi, Yeong-Jin, Park, Honghyun, Yun, Hui-suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395714/
https://www.ncbi.nlm.nih.gov/pubmed/36017354
http://dx.doi.org/10.3389/fbioe.2022.827626
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author Kim, Jueun
Choi, Yeong-Jin
Park, Honghyun
Yun, Hui-suk
author_facet Kim, Jueun
Choi, Yeong-Jin
Park, Honghyun
Yun, Hui-suk
author_sort Kim, Jueun
collection PubMed
description Novel alginate-hydroxyapatite hybrid microspheres were developed for simultaneous delivery of drugs and cells as a multifunctional bone substitute for osteoporotic bone tissue regeneration. The microspheres were used to enhance osteogenesis and to carry and deliver quercetin, a representative phytoestrogen that controls bone tissue regeneration metabolism in osteoporosis patients, through sustained release over a long period. To overcome quercetin’s hydrophobicity and low solubility in aqueous environments, we added it to the surface of hydroxyapatite (HAp) nanoparticles before mixing them with an alginate solution. The homogeneous distribution of the HAp nanoparticles in the alginate solution was essential for preventing nozzle clogging and achieving successfully fabricated hybrid microspheres. To this end, a 3D ultrasonic treatment was applied. Electrostatic microencapsulation was then used to fabricate hybrid alginate-HAp microspheres containing quercetin and cells. The microspheres were approximately 290.7 ± 42.5 μm (aspect ratio of 1). The sustained release of quercetin was confirmed during a test period of 20 weeks. The cells in the hybrid microspheres maintained good cell viability during the entire testing period, and their osteogenic differentiation behavior was boosted by the presence of HAp. Thus, osteogenic differentiation could be greatly improved by adding quercetin. These novel multi-biofunctional hybrid microspheres have great potential for the regeneration of osteoporotic bone tissue at indeterminate defect sites.
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spelling pubmed-93957142022-08-24 Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery Kim, Jueun Choi, Yeong-Jin Park, Honghyun Yun, Hui-suk Front Bioeng Biotechnol Bioengineering and Biotechnology Novel alginate-hydroxyapatite hybrid microspheres were developed for simultaneous delivery of drugs and cells as a multifunctional bone substitute for osteoporotic bone tissue regeneration. The microspheres were used to enhance osteogenesis and to carry and deliver quercetin, a representative phytoestrogen that controls bone tissue regeneration metabolism in osteoporosis patients, through sustained release over a long period. To overcome quercetin’s hydrophobicity and low solubility in aqueous environments, we added it to the surface of hydroxyapatite (HAp) nanoparticles before mixing them with an alginate solution. The homogeneous distribution of the HAp nanoparticles in the alginate solution was essential for preventing nozzle clogging and achieving successfully fabricated hybrid microspheres. To this end, a 3D ultrasonic treatment was applied. Electrostatic microencapsulation was then used to fabricate hybrid alginate-HAp microspheres containing quercetin and cells. The microspheres were approximately 290.7 ± 42.5 μm (aspect ratio of 1). The sustained release of quercetin was confirmed during a test period of 20 weeks. The cells in the hybrid microspheres maintained good cell viability during the entire testing period, and their osteogenic differentiation behavior was boosted by the presence of HAp. Thus, osteogenic differentiation could be greatly improved by adding quercetin. These novel multi-biofunctional hybrid microspheres have great potential for the regeneration of osteoporotic bone tissue at indeterminate defect sites. Frontiers Media S.A. 2022-08-09 /pmc/articles/PMC9395714/ /pubmed/36017354 http://dx.doi.org/10.3389/fbioe.2022.827626 Text en Copyright © 2022 Kim, Choi, Park and Yun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Kim, Jueun
Choi, Yeong-Jin
Park, Honghyun
Yun, Hui-suk
Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery
title Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery
title_full Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery
title_fullStr Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery
title_full_unstemmed Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery
title_short Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery
title_sort fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395714/
https://www.ncbi.nlm.nih.gov/pubmed/36017354
http://dx.doi.org/10.3389/fbioe.2022.827626
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