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Multiparametric analysis of host and parasite elements in new world tegumentary leishmaniasis
Tegumentary leishmaniasis is a tropical disease caused by protozoa of the genus Leishmania. Clinically, the disease presents a broad spectrum of symptoms, the mechanisms underlying the development of lesions remaining to be fully elucidated. In the present work, we performed a correlation and multip...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395741/ https://www.ncbi.nlm.nih.gov/pubmed/36017367 http://dx.doi.org/10.3389/fcimb.2022.956112 |
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author | de Carvalho, Bruna Caroline Vital, Tamires Osiro, Jaqueline Gomes, Ciro Martins Noronha, Elza Dallago, Bruno Rosa, Ana de Cássia Carvalho, Juliana Lott Hagström, Luciana Hecht, Mariana Nitz, Nadjar |
author_facet | de Carvalho, Bruna Caroline Vital, Tamires Osiro, Jaqueline Gomes, Ciro Martins Noronha, Elza Dallago, Bruno Rosa, Ana de Cássia Carvalho, Juliana Lott Hagström, Luciana Hecht, Mariana Nitz, Nadjar |
author_sort | de Carvalho, Bruna Caroline |
collection | PubMed |
description | Tegumentary leishmaniasis is a tropical disease caused by protozoa of the genus Leishmania. Clinically, the disease presents a broad spectrum of symptoms, the mechanisms underlying the development of lesions remaining to be fully elucidated. In the present work, we performed a correlation and multiparametric analysis to evaluate how parasite- and host-related aspects associate with each other, and with the different clinical manifestations of tegumentary leishmaniasis. This cross-sectional study involved 75 individuals from endemic areas of Brazil, grouped according to their symptoms. Leishmania species were determined by DNA sequencing, and parasite load, antibody production, and cytokine profile were evaluated by kDNA qPCR, ELISA, and flow cytometry. Data were analyzed using the Chi-square test, principal component analysis, canonical discriminant analysis, and correlation analysis. Among the recruited patients, 23 (31%) were asymptomatic, 34 (45%) had primary cutaneous leishmaniasis, 10 (13%) presented recurrent cutaneous leishmaniasis, and eight (11%) had mucocutaneous leishmaniasis. Leishmania species identified included L. amazonensis, L. braziliensis, and L. guyanensis. Surprisingly, no Leishmania RNA virus infection was detected in any sample. In summary, our work showed that parasite load, antibody production, and cytokine levels alone are not determinants for tegumentary leishmaniasis symptoms. However, the correlation analysis allowed us to observe how these factors are correlated to each other within the groups, which revealed a unique network for each clinical manifestation. Our work reinforces the complexity of tegumentary leishmaniasis outcomes - which are associated with multiple host and parasite-related elements and provides a holistic model of the disease. |
format | Online Article Text |
id | pubmed-9395741 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93957412022-08-24 Multiparametric analysis of host and parasite elements in new world tegumentary leishmaniasis de Carvalho, Bruna Caroline Vital, Tamires Osiro, Jaqueline Gomes, Ciro Martins Noronha, Elza Dallago, Bruno Rosa, Ana de Cássia Carvalho, Juliana Lott Hagström, Luciana Hecht, Mariana Nitz, Nadjar Front Cell Infect Microbiol Cellular and Infection Microbiology Tegumentary leishmaniasis is a tropical disease caused by protozoa of the genus Leishmania. Clinically, the disease presents a broad spectrum of symptoms, the mechanisms underlying the development of lesions remaining to be fully elucidated. In the present work, we performed a correlation and multiparametric analysis to evaluate how parasite- and host-related aspects associate with each other, and with the different clinical manifestations of tegumentary leishmaniasis. This cross-sectional study involved 75 individuals from endemic areas of Brazil, grouped according to their symptoms. Leishmania species were determined by DNA sequencing, and parasite load, antibody production, and cytokine profile were evaluated by kDNA qPCR, ELISA, and flow cytometry. Data were analyzed using the Chi-square test, principal component analysis, canonical discriminant analysis, and correlation analysis. Among the recruited patients, 23 (31%) were asymptomatic, 34 (45%) had primary cutaneous leishmaniasis, 10 (13%) presented recurrent cutaneous leishmaniasis, and eight (11%) had mucocutaneous leishmaniasis. Leishmania species identified included L. amazonensis, L. braziliensis, and L. guyanensis. Surprisingly, no Leishmania RNA virus infection was detected in any sample. In summary, our work showed that parasite load, antibody production, and cytokine levels alone are not determinants for tegumentary leishmaniasis symptoms. However, the correlation analysis allowed us to observe how these factors are correlated to each other within the groups, which revealed a unique network for each clinical manifestation. Our work reinforces the complexity of tegumentary leishmaniasis outcomes - which are associated with multiple host and parasite-related elements and provides a holistic model of the disease. Frontiers Media S.A. 2022-08-09 /pmc/articles/PMC9395741/ /pubmed/36017367 http://dx.doi.org/10.3389/fcimb.2022.956112 Text en Copyright © 2022 de Carvalho, Vital, Osiro, Gomes, Noronha, Dallago, Rosa, Carvalho, Hagström, Hecht and Nitz https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology de Carvalho, Bruna Caroline Vital, Tamires Osiro, Jaqueline Gomes, Ciro Martins Noronha, Elza Dallago, Bruno Rosa, Ana de Cássia Carvalho, Juliana Lott Hagström, Luciana Hecht, Mariana Nitz, Nadjar Multiparametric analysis of host and parasite elements in new world tegumentary leishmaniasis |
title | Multiparametric analysis of host and parasite elements in new world tegumentary leishmaniasis |
title_full | Multiparametric analysis of host and parasite elements in new world tegumentary leishmaniasis |
title_fullStr | Multiparametric analysis of host and parasite elements in new world tegumentary leishmaniasis |
title_full_unstemmed | Multiparametric analysis of host and parasite elements in new world tegumentary leishmaniasis |
title_short | Multiparametric analysis of host and parasite elements in new world tegumentary leishmaniasis |
title_sort | multiparametric analysis of host and parasite elements in new world tegumentary leishmaniasis |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395741/ https://www.ncbi.nlm.nih.gov/pubmed/36017367 http://dx.doi.org/10.3389/fcimb.2022.956112 |
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