Whole-cell FRET monitoring of transcription factor activities enables functional annotation of signal transduction systems in living bacteria

Bacteria adapt to their constantly changing environments largely by transcriptional regulation through the activities of various transcription factors (TFs). However, techniques that monitor TF–promoter interactions in situ in living bacteria are lacking. Herein, we developed a whole-cell TF–promote...

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Detalles Bibliográficos
Autores principales: Wang, Pengchao, Zhang, Guangming, Xu, Zeling, Chen, Zhe, Liu, Xiaohong, Wang, Chenyin, Zheng, Chaogu, Wang, Jiangyun, Zhang, Hongmin, Yan, Aixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396075/
https://www.ncbi.nlm.nih.gov/pubmed/35839853
http://dx.doi.org/10.1016/j.jbc.2022.102258

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