Cargando…

Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China

This study aimed to investigate the prevalence and characterization of tet(X4) in Escherichia coli isolates from a pig farm in Shanghai, China, and to elucidate tet(X4) dissemination mechanism in this swine farm. Forty-nine (80.33%) E. coli strains were isolated from 61 samples from a pig farm and w...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jing, Lu, Meng-Jun, Wang, Zhen-Yu, Jiang, Yue, Wu, Han, Pan, Zhi-Ming, Jiao, Xinan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396132/
https://www.ncbi.nlm.nih.gov/pubmed/36016796
http://dx.doi.org/10.3389/fmicb.2022.967313
_version_ 1784771861808152576
author Wang, Jing
Lu, Meng-Jun
Wang, Zhen-Yu
Jiang, Yue
Wu, Han
Pan, Zhi-Ming
Jiao, Xinan
author_facet Wang, Jing
Lu, Meng-Jun
Wang, Zhen-Yu
Jiang, Yue
Wu, Han
Pan, Zhi-Ming
Jiao, Xinan
author_sort Wang, Jing
collection PubMed
description This study aimed to investigate the prevalence and characterization of tet(X4) in Escherichia coli isolates from a pig farm in Shanghai, China, and to elucidate tet(X4) dissemination mechanism in this swine farm. Forty-nine (80.33%) E. coli strains were isolated from 61 samples from a pig farm and were screened for the presence of tet(X). Among them, six (12.24%) strains were positive for tet(X4) and exhibited resistance to tigecycline (MIC ≥ 16 mg/L). They were further sequenced by Illumina Hiseq. Six tet(X4)-positive strains belonged to ST761 with identical resistance genes, resistance profiles, plasmid replicons, and cgMLST type except that additional ColE10 plasmid was present in isolate SH21PTE35. Isolate SH21PTE31, as a representative ST761 E. coli strain, was further sequenced using Nanopore MinION. The tet(X4) in SH21PTE31 was located on IncFIA18/IncFIB(K)/IncX1 hybrid plasmid pYUSHP31-1, highly similar to other tet(X4)-carrying IncFIA18/IncFIB(K)/IncX1 plasmids from ST761 E. coli and other E. coli lineages in China. These IncFIA18/IncFIB(K)/IncX1 plasmids shared closely related multidrug resistance regions, and could reorganize, acquire or lose resistance modules mediated by mobile elements such as ISCR2 and IS26. Phylogenetic analysis were performed including all tet(X4)-positive isolates obtained in this pig farm combined with 43 tet(X4)-positive E. coli from pigs, cow, pork, wastewater, and patients with the same ST from NCBI. The 50 tet(X4)-carrying E. coli ST761 isolates from different areas in China shared a close phylogenetic relationship (0-49 SNPs). In conclusion, clonal transmission of tet(X4)-positive E. coli ST761 has occurred in this swine farm. E. coli ST761 has the potential to become a high-risk clone for tet(X4) dissemination in China.
format Online
Article
Text
id pubmed-9396132
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93961322022-08-24 Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China Wang, Jing Lu, Meng-Jun Wang, Zhen-Yu Jiang, Yue Wu, Han Pan, Zhi-Ming Jiao, Xinan Front Microbiol Microbiology This study aimed to investigate the prevalence and characterization of tet(X4) in Escherichia coli isolates from a pig farm in Shanghai, China, and to elucidate tet(X4) dissemination mechanism in this swine farm. Forty-nine (80.33%) E. coli strains were isolated from 61 samples from a pig farm and were screened for the presence of tet(X). Among them, six (12.24%) strains were positive for tet(X4) and exhibited resistance to tigecycline (MIC ≥ 16 mg/L). They were further sequenced by Illumina Hiseq. Six tet(X4)-positive strains belonged to ST761 with identical resistance genes, resistance profiles, plasmid replicons, and cgMLST type except that additional ColE10 plasmid was present in isolate SH21PTE35. Isolate SH21PTE31, as a representative ST761 E. coli strain, was further sequenced using Nanopore MinION. The tet(X4) in SH21PTE31 was located on IncFIA18/IncFIB(K)/IncX1 hybrid plasmid pYUSHP31-1, highly similar to other tet(X4)-carrying IncFIA18/IncFIB(K)/IncX1 plasmids from ST761 E. coli and other E. coli lineages in China. These IncFIA18/IncFIB(K)/IncX1 plasmids shared closely related multidrug resistance regions, and could reorganize, acquire or lose resistance modules mediated by mobile elements such as ISCR2 and IS26. Phylogenetic analysis were performed including all tet(X4)-positive isolates obtained in this pig farm combined with 43 tet(X4)-positive E. coli from pigs, cow, pork, wastewater, and patients with the same ST from NCBI. The 50 tet(X4)-carrying E. coli ST761 isolates from different areas in China shared a close phylogenetic relationship (0-49 SNPs). In conclusion, clonal transmission of tet(X4)-positive E. coli ST761 has occurred in this swine farm. E. coli ST761 has the potential to become a high-risk clone for tet(X4) dissemination in China. Frontiers Media S.A. 2022-08-09 /pmc/articles/PMC9396132/ /pubmed/36016796 http://dx.doi.org/10.3389/fmicb.2022.967313 Text en Copyright © 2022 Wang, Lu, Wang, Jiang, Wu, Pan and Jiao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Jing
Lu, Meng-Jun
Wang, Zhen-Yu
Jiang, Yue
Wu, Han
Pan, Zhi-Ming
Jiao, Xinan
Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China
title Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China
title_full Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China
title_fullStr Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China
title_full_unstemmed Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China
title_short Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China
title_sort tigecycline-resistant escherichia coli st761 carrying tet(x4) in a pig farm, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396132/
https://www.ncbi.nlm.nih.gov/pubmed/36016796
http://dx.doi.org/10.3389/fmicb.2022.967313
work_keys_str_mv AT wangjing tigecyclineresistantescherichiacolist761carryingtetx4inapigfarmchina
AT lumengjun tigecyclineresistantescherichiacolist761carryingtetx4inapigfarmchina
AT wangzhenyu tigecyclineresistantescherichiacolist761carryingtetx4inapigfarmchina
AT jiangyue tigecyclineresistantescherichiacolist761carryingtetx4inapigfarmchina
AT wuhan tigecyclineresistantescherichiacolist761carryingtetx4inapigfarmchina
AT panzhiming tigecyclineresistantescherichiacolist761carryingtetx4inapigfarmchina
AT jiaoxinan tigecyclineresistantescherichiacolist761carryingtetx4inapigfarmchina