Cargando…

Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cells

Lymphocytes can heavily influence intestinal health, but resolving intestinal lymphocyte function is challenging as the intestine contains a vastly heterogeneous mixture of cells. Pigs are an advantageous biomedical model, but deeper understanding of intestinal lymphocytes is warranted to improve mo...

Descripción completa

Detalles Bibliográficos
Autores principales: Wiarda, Jayne E, Trachsel, Julian M, Sivasankaran, Sathesh K, Tuggle, Christopher K, Loving, Crystal L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396248/
https://www.ncbi.nlm.nih.gov/pubmed/35995567
http://dx.doi.org/10.26508/lsa.202201442
_version_ 1784771888113778688
author Wiarda, Jayne E
Trachsel, Julian M
Sivasankaran, Sathesh K
Tuggle, Christopher K
Loving, Crystal L
author_facet Wiarda, Jayne E
Trachsel, Julian M
Sivasankaran, Sathesh K
Tuggle, Christopher K
Loving, Crystal L
author_sort Wiarda, Jayne E
collection PubMed
description Lymphocytes can heavily influence intestinal health, but resolving intestinal lymphocyte function is challenging as the intestine contains a vastly heterogeneous mixture of cells. Pigs are an advantageous biomedical model, but deeper understanding of intestinal lymphocytes is warranted to improve model utility. Twenty-six cell types were identified in the porcine ileum by single-cell RNA sequencing and further compared with cells in human and murine ileum. Though general consensus of cell subsets across species was revealed, some porcine-specific lymphocyte subsets were identified. Differential tissue dissection and in situ analyses conferred spatial context, revealing similar locations of lymphocyte subsets in Peyer’s patches and epithelium in pig-to-human comparisons. Like humans, activated and effector lymphocytes were abundant in the ileum but not periphery of pigs, suggesting tissue-specific and/or activation-associated gene expression. Gene signatures for peripheral and ileal innate lymphoid cells newly discovered in pigs were defined and highlighted similarities to human innate lymphoid cells. Overall, we reveal novel lymphocyte subsets in pigs and highlight utility of pigs for intestinal research applications.
format Online
Article
Text
id pubmed-9396248
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Life Science Alliance LLC
record_format MEDLINE/PubMed
spelling pubmed-93962482022-09-02 Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cells Wiarda, Jayne E Trachsel, Julian M Sivasankaran, Sathesh K Tuggle, Christopher K Loving, Crystal L Life Sci Alliance Resources Lymphocytes can heavily influence intestinal health, but resolving intestinal lymphocyte function is challenging as the intestine contains a vastly heterogeneous mixture of cells. Pigs are an advantageous biomedical model, but deeper understanding of intestinal lymphocytes is warranted to improve model utility. Twenty-six cell types were identified in the porcine ileum by single-cell RNA sequencing and further compared with cells in human and murine ileum. Though general consensus of cell subsets across species was revealed, some porcine-specific lymphocyte subsets were identified. Differential tissue dissection and in situ analyses conferred spatial context, revealing similar locations of lymphocyte subsets in Peyer’s patches and epithelium in pig-to-human comparisons. Like humans, activated and effector lymphocytes were abundant in the ileum but not periphery of pigs, suggesting tissue-specific and/or activation-associated gene expression. Gene signatures for peripheral and ileal innate lymphoid cells newly discovered in pigs were defined and highlighted similarities to human innate lymphoid cells. Overall, we reveal novel lymphocyte subsets in pigs and highlight utility of pigs for intestinal research applications. Life Science Alliance LLC 2022-08-22 /pmc/articles/PMC9396248/ /pubmed/35995567 http://dx.doi.org/10.26508/lsa.202201442 Text en © 2022 Wiarda et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Resources
Wiarda, Jayne E
Trachsel, Julian M
Sivasankaran, Sathesh K
Tuggle, Christopher K
Loving, Crystal L
Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cells
title Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cells
title_full Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cells
title_fullStr Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cells
title_full_unstemmed Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cells
title_short Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cells
title_sort intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cells
topic Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396248/
https://www.ncbi.nlm.nih.gov/pubmed/35995567
http://dx.doi.org/10.26508/lsa.202201442
work_keys_str_mv AT wiardajaynee intestinalsinglecellatlasrevealsnovellymphocytesinpigswithsimilaritiestohumancells
AT trachseljulianm intestinalsinglecellatlasrevealsnovellymphocytesinpigswithsimilaritiestohumancells
AT sivasankaransatheshk intestinalsinglecellatlasrevealsnovellymphocytesinpigswithsimilaritiestohumancells
AT tugglechristopherk intestinalsinglecellatlasrevealsnovellymphocytesinpigswithsimilaritiestohumancells
AT lovingcrystall intestinalsinglecellatlasrevealsnovellymphocytesinpigswithsimilaritiestohumancells