A cAMP Sensor Based on Ligand-Dependent Protein Stabilization

[Image: see text] cAMP is a ubiquitous second messenger with many functions in diverse organisms. Current cAMP sensors, including Föster resonance energy transfer (FRET)-based and single-wavelength-based sensors, allow for real time visualization of this small molecule in cultured cells and in some...

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Autores principales: Sidoli, Mariapaola, Chen, Ling-chun, Lu, Alexander J., Wandless, Thomas J., Talbot, William S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396618/
https://www.ncbi.nlm.nih.gov/pubmed/35839076
http://dx.doi.org/10.1021/acschembio.2c00333
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author Sidoli, Mariapaola
Chen, Ling-chun
Lu, Alexander J.
Wandless, Thomas J.
Talbot, William S.
author_facet Sidoli, Mariapaola
Chen, Ling-chun
Lu, Alexander J.
Wandless, Thomas J.
Talbot, William S.
author_sort Sidoli, Mariapaola
collection PubMed
description [Image: see text] cAMP is a ubiquitous second messenger with many functions in diverse organisms. Current cAMP sensors, including Föster resonance energy transfer (FRET)-based and single-wavelength-based sensors, allow for real time visualization of this small molecule in cultured cells and in some cases in vivo. Nonetheless the observation of cAMP in living animals is still difficult, typically requiring specialized microscopes and ex vivo tissue processing. Here we used ligand-dependent protein stabilization to create a new cAMP sensor. This sensor allows specific and sensitive detection of cAMP in living zebrafish embryos, which may enable new understanding of the functions of cAMP in living vertebrates.
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spelling pubmed-93966182022-08-24 A cAMP Sensor Based on Ligand-Dependent Protein Stabilization Sidoli, Mariapaola Chen, Ling-chun Lu, Alexander J. Wandless, Thomas J. Talbot, William S. ACS Chem Biol [Image: see text] cAMP is a ubiquitous second messenger with many functions in diverse organisms. Current cAMP sensors, including Föster resonance energy transfer (FRET)-based and single-wavelength-based sensors, allow for real time visualization of this small molecule in cultured cells and in some cases in vivo. Nonetheless the observation of cAMP in living animals is still difficult, typically requiring specialized microscopes and ex vivo tissue processing. Here we used ligand-dependent protein stabilization to create a new cAMP sensor. This sensor allows specific and sensitive detection of cAMP in living zebrafish embryos, which may enable new understanding of the functions of cAMP in living vertebrates. American Chemical Society 2022-07-15 2022-08-19 /pmc/articles/PMC9396618/ /pubmed/35839076 http://dx.doi.org/10.1021/acschembio.2c00333 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Sidoli, Mariapaola
Chen, Ling-chun
Lu, Alexander J.
Wandless, Thomas J.
Talbot, William S.
A cAMP Sensor Based on Ligand-Dependent Protein Stabilization
title A cAMP Sensor Based on Ligand-Dependent Protein Stabilization
title_full A cAMP Sensor Based on Ligand-Dependent Protein Stabilization
title_fullStr A cAMP Sensor Based on Ligand-Dependent Protein Stabilization
title_full_unstemmed A cAMP Sensor Based on Ligand-Dependent Protein Stabilization
title_short A cAMP Sensor Based on Ligand-Dependent Protein Stabilization
title_sort camp sensor based on ligand-dependent protein stabilization
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396618/
https://www.ncbi.nlm.nih.gov/pubmed/35839076
http://dx.doi.org/10.1021/acschembio.2c00333
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