Cargando…
Green synthesis of biocompatible core–shell (Au–Ag) and hybrid (Au–ZnO and Ag–ZnO) bimetallic nanoparticles and evaluation of their potential antibacterial, antidiabetic, antiglycation and anticancer activities
The fabrication of bimetallic nanoparticles (BNPs) using plant extracts is applauded since it is an environmentally and biologically safe method. In this research, Manilkara zapota leaf extract was utilized to bioreduce metal ions for the production of therapeutically important core–shell Au–Ag and...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396731/ https://www.ncbi.nlm.nih.gov/pubmed/36093232 http://dx.doi.org/10.1039/d2ra03196e |
_version_ | 1784771988748763136 |
---|---|
author | Anjum, Sumaira Nawaz, Khadija Ahmad, Bushra Hano, Christophe Abbasi, Bilal Haider |
author_facet | Anjum, Sumaira Nawaz, Khadija Ahmad, Bushra Hano, Christophe Abbasi, Bilal Haider |
author_sort | Anjum, Sumaira |
collection | PubMed |
description | The fabrication of bimetallic nanoparticles (BNPs) using plant extracts is applauded since it is an environmentally and biologically safe method. In this research, Manilkara zapota leaf extract was utilized to bioreduce metal ions for the production of therapeutically important core–shell Au–Ag and hybrid (Au–ZnO and Ag–ZnO) BNPs. The phytochemical profiling of the leaf extract in terms of total phenolic and flavonoid content is attributed to its high free radical scavenging activity. FTIR data also supported the involvement of these phytochemicals (polyphenols, flavonoids, aromatic compounds and alkynes) in the synthesis of BNPs. Whereas, TEM and XRD showed the formation of small sized (16.57 nm) spherical shaped core–shell Au–Ag BNPs and ZnO nano-needles with spherical AuNPs (48.32 nm) and ZnO nano-rods with spherical AgNP (19.64 nm) hybrid BNPs. The biological activities of BNPs reinforced the fact that they show enhanced therapeutic efficacy as compared to their monometallic components. All BNPs showed comparable antibacterial activities as compared to standard tetracycline discs. While small sized Au–Ag BNPs were most effective in killing human hepato-cellular carcinoma cells (HepG2) in terms of lowest cell viability, highest intracellular ROS/RNS production, loss of mitochondrial membrane potential, induction of caspase-3 gene expression and enhanced caspase-3/7 activity. BNPs also effectively inhibited advanced glycation end products and carbohydrate digesting enzymes which can be used as a nano-medicine for aging and diabetes. The most important finding was the permissible biocompatibility of these BNPs towards brine shrimp larvae and human RBCs, which suggests their environmental and biological safety. This research study gives us insight into the promise of using a green route to synthesize commercially important BNPs with enhanced therapeutic efficacy as compared to conventional treatment options. |
format | Online Article Text |
id | pubmed-9396731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-93967312022-09-08 Green synthesis of biocompatible core–shell (Au–Ag) and hybrid (Au–ZnO and Ag–ZnO) bimetallic nanoparticles and evaluation of their potential antibacterial, antidiabetic, antiglycation and anticancer activities Anjum, Sumaira Nawaz, Khadija Ahmad, Bushra Hano, Christophe Abbasi, Bilal Haider RSC Adv Chemistry The fabrication of bimetallic nanoparticles (BNPs) using plant extracts is applauded since it is an environmentally and biologically safe method. In this research, Manilkara zapota leaf extract was utilized to bioreduce metal ions for the production of therapeutically important core–shell Au–Ag and hybrid (Au–ZnO and Ag–ZnO) BNPs. The phytochemical profiling of the leaf extract in terms of total phenolic and flavonoid content is attributed to its high free radical scavenging activity. FTIR data also supported the involvement of these phytochemicals (polyphenols, flavonoids, aromatic compounds and alkynes) in the synthesis of BNPs. Whereas, TEM and XRD showed the formation of small sized (16.57 nm) spherical shaped core–shell Au–Ag BNPs and ZnO nano-needles with spherical AuNPs (48.32 nm) and ZnO nano-rods with spherical AgNP (19.64 nm) hybrid BNPs. The biological activities of BNPs reinforced the fact that they show enhanced therapeutic efficacy as compared to their monometallic components. All BNPs showed comparable antibacterial activities as compared to standard tetracycline discs. While small sized Au–Ag BNPs were most effective in killing human hepato-cellular carcinoma cells (HepG2) in terms of lowest cell viability, highest intracellular ROS/RNS production, loss of mitochondrial membrane potential, induction of caspase-3 gene expression and enhanced caspase-3/7 activity. BNPs also effectively inhibited advanced glycation end products and carbohydrate digesting enzymes which can be used as a nano-medicine for aging and diabetes. The most important finding was the permissible biocompatibility of these BNPs towards brine shrimp larvae and human RBCs, which suggests their environmental and biological safety. This research study gives us insight into the promise of using a green route to synthesize commercially important BNPs with enhanced therapeutic efficacy as compared to conventional treatment options. The Royal Society of Chemistry 2022-08-23 /pmc/articles/PMC9396731/ /pubmed/36093232 http://dx.doi.org/10.1039/d2ra03196e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Anjum, Sumaira Nawaz, Khadija Ahmad, Bushra Hano, Christophe Abbasi, Bilal Haider Green synthesis of biocompatible core–shell (Au–Ag) and hybrid (Au–ZnO and Ag–ZnO) bimetallic nanoparticles and evaluation of their potential antibacterial, antidiabetic, antiglycation and anticancer activities |
title | Green synthesis of biocompatible core–shell (Au–Ag) and hybrid (Au–ZnO and Ag–ZnO) bimetallic nanoparticles and evaluation of their potential antibacterial, antidiabetic, antiglycation and anticancer activities |
title_full | Green synthesis of biocompatible core–shell (Au–Ag) and hybrid (Au–ZnO and Ag–ZnO) bimetallic nanoparticles and evaluation of their potential antibacterial, antidiabetic, antiglycation and anticancer activities |
title_fullStr | Green synthesis of biocompatible core–shell (Au–Ag) and hybrid (Au–ZnO and Ag–ZnO) bimetallic nanoparticles and evaluation of their potential antibacterial, antidiabetic, antiglycation and anticancer activities |
title_full_unstemmed | Green synthesis of biocompatible core–shell (Au–Ag) and hybrid (Au–ZnO and Ag–ZnO) bimetallic nanoparticles and evaluation of their potential antibacterial, antidiabetic, antiglycation and anticancer activities |
title_short | Green synthesis of biocompatible core–shell (Au–Ag) and hybrid (Au–ZnO and Ag–ZnO) bimetallic nanoparticles and evaluation of their potential antibacterial, antidiabetic, antiglycation and anticancer activities |
title_sort | green synthesis of biocompatible core–shell (au–ag) and hybrid (au–zno and ag–zno) bimetallic nanoparticles and evaluation of their potential antibacterial, antidiabetic, antiglycation and anticancer activities |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396731/ https://www.ncbi.nlm.nih.gov/pubmed/36093232 http://dx.doi.org/10.1039/d2ra03196e |
work_keys_str_mv | AT anjumsumaira greensynthesisofbiocompatiblecoreshellauagandhybridauznoandagznobimetallicnanoparticlesandevaluationoftheirpotentialantibacterialantidiabeticantiglycationandanticanceractivities AT nawazkhadija greensynthesisofbiocompatiblecoreshellauagandhybridauznoandagznobimetallicnanoparticlesandevaluationoftheirpotentialantibacterialantidiabeticantiglycationandanticanceractivities AT ahmadbushra greensynthesisofbiocompatiblecoreshellauagandhybridauznoandagznobimetallicnanoparticlesandevaluationoftheirpotentialantibacterialantidiabeticantiglycationandanticanceractivities AT hanochristophe greensynthesisofbiocompatiblecoreshellauagandhybridauznoandagznobimetallicnanoparticlesandevaluationoftheirpotentialantibacterialantidiabeticantiglycationandanticanceractivities AT abbasibilalhaider greensynthesisofbiocompatiblecoreshellauagandhybridauznoandagznobimetallicnanoparticlesandevaluationoftheirpotentialantibacterialantidiabeticantiglycationandanticanceractivities |