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Effect of Cellulose–Chitosan Hybrid-Based Encapsulation on the Viability and Stability of Probiotics under Simulated Gastric Transit and in Kefir

Encapsulation comprises a promising potential for the targeted delivery of entrapped sensitive agents into the food system. A unique combination of cellulose/chitosan (Cl-Ch)-based hybrid wall material was employed to encapsulate L. plantarum by emulsion technique. The developed beads were further s...

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Autores principales: Afzaal, Muhammad, Saeed, Farhan, Ateeq, Huda, Shah, Yasir Abbas, Hussain, Muzzamal, Javed, Ahsan, Ikram, Ali, Raza, Muhammad Ahtisham, Nayik, Gulzar Ahmad, Alfarraj, Saleh, Ansari, Mohammad Javed, Karabagias, Ioannis K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9397047/
https://www.ncbi.nlm.nih.gov/pubmed/35997429
http://dx.doi.org/10.3390/biomimetics7030109
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author Afzaal, Muhammad
Saeed, Farhan
Ateeq, Huda
Shah, Yasir Abbas
Hussain, Muzzamal
Javed, Ahsan
Ikram, Ali
Raza, Muhammad Ahtisham
Nayik, Gulzar Ahmad
Alfarraj, Saleh
Ansari, Mohammad Javed
Karabagias, Ioannis K.
author_facet Afzaal, Muhammad
Saeed, Farhan
Ateeq, Huda
Shah, Yasir Abbas
Hussain, Muzzamal
Javed, Ahsan
Ikram, Ali
Raza, Muhammad Ahtisham
Nayik, Gulzar Ahmad
Alfarraj, Saleh
Ansari, Mohammad Javed
Karabagias, Ioannis K.
author_sort Afzaal, Muhammad
collection PubMed
description Encapsulation comprises a promising potential for the targeted delivery of entrapped sensitive agents into the food system. A unique combination of cellulose/chitosan (Cl-Ch)-based hybrid wall material was employed to encapsulate L. plantarum by emulsion technique. The developed beads were further subjected to morphological and in vitro studies. The viability of free and encapsulated probiotics was also evaluated in kefir during storage. The developed beads presented porous spherical structures with a rough surface. A 1.58 ± 0.02 log CFU/mL, 1.26 ± 0.01 log CFU/mL, and 1.82 ± 0.01 log CFU/mL reduction were noticed for Cl-Ch hybrid cells under simulated gastro-intestinal and thermal conditions, respectively. The encapsulated cells were found to be acidic and thermally resistant compared to the free cells. Similarly, encapsulated probiotics showed better viability in kefir at the end of the storage period compared to free cells. In short, the newly developed Cl-Ch hybrid-based encapsulation has a promising potential for the targeted delivery of probiotics, as career agents, in gastric transit, and in foods.
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spelling pubmed-93970472022-08-24 Effect of Cellulose–Chitosan Hybrid-Based Encapsulation on the Viability and Stability of Probiotics under Simulated Gastric Transit and in Kefir Afzaal, Muhammad Saeed, Farhan Ateeq, Huda Shah, Yasir Abbas Hussain, Muzzamal Javed, Ahsan Ikram, Ali Raza, Muhammad Ahtisham Nayik, Gulzar Ahmad Alfarraj, Saleh Ansari, Mohammad Javed Karabagias, Ioannis K. Biomimetics (Basel) Article Encapsulation comprises a promising potential for the targeted delivery of entrapped sensitive agents into the food system. A unique combination of cellulose/chitosan (Cl-Ch)-based hybrid wall material was employed to encapsulate L. plantarum by emulsion technique. The developed beads were further subjected to morphological and in vitro studies. The viability of free and encapsulated probiotics was also evaluated in kefir during storage. The developed beads presented porous spherical structures with a rough surface. A 1.58 ± 0.02 log CFU/mL, 1.26 ± 0.01 log CFU/mL, and 1.82 ± 0.01 log CFU/mL reduction were noticed for Cl-Ch hybrid cells under simulated gastro-intestinal and thermal conditions, respectively. The encapsulated cells were found to be acidic and thermally resistant compared to the free cells. Similarly, encapsulated probiotics showed better viability in kefir at the end of the storage period compared to free cells. In short, the newly developed Cl-Ch hybrid-based encapsulation has a promising potential for the targeted delivery of probiotics, as career agents, in gastric transit, and in foods. MDPI 2022-08-10 /pmc/articles/PMC9397047/ /pubmed/35997429 http://dx.doi.org/10.3390/biomimetics7030109 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Afzaal, Muhammad
Saeed, Farhan
Ateeq, Huda
Shah, Yasir Abbas
Hussain, Muzzamal
Javed, Ahsan
Ikram, Ali
Raza, Muhammad Ahtisham
Nayik, Gulzar Ahmad
Alfarraj, Saleh
Ansari, Mohammad Javed
Karabagias, Ioannis K.
Effect of Cellulose–Chitosan Hybrid-Based Encapsulation on the Viability and Stability of Probiotics under Simulated Gastric Transit and in Kefir
title Effect of Cellulose–Chitosan Hybrid-Based Encapsulation on the Viability and Stability of Probiotics under Simulated Gastric Transit and in Kefir
title_full Effect of Cellulose–Chitosan Hybrid-Based Encapsulation on the Viability and Stability of Probiotics under Simulated Gastric Transit and in Kefir
title_fullStr Effect of Cellulose–Chitosan Hybrid-Based Encapsulation on the Viability and Stability of Probiotics under Simulated Gastric Transit and in Kefir
title_full_unstemmed Effect of Cellulose–Chitosan Hybrid-Based Encapsulation on the Viability and Stability of Probiotics under Simulated Gastric Transit and in Kefir
title_short Effect of Cellulose–Chitosan Hybrid-Based Encapsulation on the Viability and Stability of Probiotics under Simulated Gastric Transit and in Kefir
title_sort effect of cellulose–chitosan hybrid-based encapsulation on the viability and stability of probiotics under simulated gastric transit and in kefir
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9397047/
https://www.ncbi.nlm.nih.gov/pubmed/35997429
http://dx.doi.org/10.3390/biomimetics7030109
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