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Assembly of root-associated N(2)O-reducing communities of annual crops is governed by selection for nosZ clade I over clade II

The rhizosphere is a hotspot for denitrification. The nitrous oxide (N(2)O) reductase among denitrifiers and nondenitrifying N(2)O reducers is the only known N(2)O sink in the biosphere. We hypothesized that the composition of root-associated N(2)O-reducing communities when establishing on annual cr...

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Autores principales: Graf, Daniel R H, Jones, Christopher M, Zhao, Ming, Hallin, Sara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9397574/
https://www.ncbi.nlm.nih.gov/pubmed/35927461
http://dx.doi.org/10.1093/femsec/fiac092
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author Graf, Daniel R H
Jones, Christopher M
Zhao, Ming
Hallin, Sara
author_facet Graf, Daniel R H
Jones, Christopher M
Zhao, Ming
Hallin, Sara
author_sort Graf, Daniel R H
collection PubMed
description The rhizosphere is a hotspot for denitrification. The nitrous oxide (N(2)O) reductase among denitrifiers and nondenitrifying N(2)O reducers is the only known N(2)O sink in the biosphere. We hypothesized that the composition of root-associated N(2)O-reducing communities when establishing on annual crops depend on soil type and plant species, but that assembly processes are independent of these factors and differ between nosZ clades I and II. Using a pot experiment with barley and sunflower and two soils, we analyzed the abundance, composition, and diversity of soil and root-associated N(2)O reducing communities by qPCR and amplicon sequencing of nosZ. Clade I was more abundant on roots compared to soil, while clade II showed the opposite. In barley, this pattern coincided with N(2)O availability, determined as potential N(2)O production rates, but for sunflower no N(2)O production was detected in the root compartment. Root and soil nosZ communities differed in composition and phylogeny-based community analyses indicated that assembly of root-associated N(2)O reducers was driven by the interaction between plant and soil type, with inferred competition being more influential than habitat selection. Selection between clades I and II in the root/soil interface is suggested, which may have functional consequences since most clade I microorganisms can produce N(2)O.
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spelling pubmed-93975742022-08-24 Assembly of root-associated N(2)O-reducing communities of annual crops is governed by selection for nosZ clade I over clade II Graf, Daniel R H Jones, Christopher M Zhao, Ming Hallin, Sara FEMS Microbiol Ecol Research Article The rhizosphere is a hotspot for denitrification. The nitrous oxide (N(2)O) reductase among denitrifiers and nondenitrifying N(2)O reducers is the only known N(2)O sink in the biosphere. We hypothesized that the composition of root-associated N(2)O-reducing communities when establishing on annual crops depend on soil type and plant species, but that assembly processes are independent of these factors and differ between nosZ clades I and II. Using a pot experiment with barley and sunflower and two soils, we analyzed the abundance, composition, and diversity of soil and root-associated N(2)O reducing communities by qPCR and amplicon sequencing of nosZ. Clade I was more abundant on roots compared to soil, while clade II showed the opposite. In barley, this pattern coincided with N(2)O availability, determined as potential N(2)O production rates, but for sunflower no N(2)O production was detected in the root compartment. Root and soil nosZ communities differed in composition and phylogeny-based community analyses indicated that assembly of root-associated N(2)O reducers was driven by the interaction between plant and soil type, with inferred competition being more influential than habitat selection. Selection between clades I and II in the root/soil interface is suggested, which may have functional consequences since most clade I microorganisms can produce N(2)O. Oxford University Press 2022-08-04 /pmc/articles/PMC9397574/ /pubmed/35927461 http://dx.doi.org/10.1093/femsec/fiac092 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Graf, Daniel R H
Jones, Christopher M
Zhao, Ming
Hallin, Sara
Assembly of root-associated N(2)O-reducing communities of annual crops is governed by selection for nosZ clade I over clade II
title Assembly of root-associated N(2)O-reducing communities of annual crops is governed by selection for nosZ clade I over clade II
title_full Assembly of root-associated N(2)O-reducing communities of annual crops is governed by selection for nosZ clade I over clade II
title_fullStr Assembly of root-associated N(2)O-reducing communities of annual crops is governed by selection for nosZ clade I over clade II
title_full_unstemmed Assembly of root-associated N(2)O-reducing communities of annual crops is governed by selection for nosZ clade I over clade II
title_short Assembly of root-associated N(2)O-reducing communities of annual crops is governed by selection for nosZ clade I over clade II
title_sort assembly of root-associated n(2)o-reducing communities of annual crops is governed by selection for nosz clade i over clade ii
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9397574/
https://www.ncbi.nlm.nih.gov/pubmed/35927461
http://dx.doi.org/10.1093/femsec/fiac092
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