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First national-scale evaluation of temephos resistance in Aedes aegypti in Peru
BACKGROUND: The development of resistance against insecticides in Aedes aegypti can lead to operational failures in control programs. Knowledge of the spatial and temporal trends of this resistance is needed to drive effective monitoring campaigns, which in turn provide data on which vector control...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9397858/ https://www.ncbi.nlm.nih.gov/pubmed/35818063 http://dx.doi.org/10.1186/s13071-022-05310-x |
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author | Palomino, Miriam Pinto, Jesus Yañez, Pamela Cornelio, Anali Dias, Luciana Amorim, Quesia Martins, Ademir Jesus Lenhart, Audrey Lima, Jose Bento Pereira |
author_facet | Palomino, Miriam Pinto, Jesus Yañez, Pamela Cornelio, Anali Dias, Luciana Amorim, Quesia Martins, Ademir Jesus Lenhart, Audrey Lima, Jose Bento Pereira |
author_sort | Palomino, Miriam |
collection | PubMed |
description | BACKGROUND: The development of resistance against insecticides in Aedes aegypti can lead to operational failures in control programs. Knowledge of the spatial and temporal trends of this resistance is needed to drive effective monitoring campaigns, which in turn provide data on which vector control decision-making should be based. METHODS: Third-stage larvae (L3) from the F1 and F2 generations of 39 Peruvian field populations of Ae. aegypti mosquitoes from established laboratory colonies were evaluated for resistance against the organophosphate insecticide temephos. The 39 populations were originally established from eggs collected in the field with ovitraps in eight departments of Peru during 2018 and 2019. Dose–response bioassays, at 11 concentrations of the insecticide, were performed following WHO recommendations. RESULTS: Of the 39 field populations of Ae. aegypti tested for resistance to temephos , 11 showed high levels of resistance (resistance ratio [RR] > 10), 16 showed moderate levels of resistance (defined as RR values between 5 and 10) and only 12 were susceptible (RR < 5). The results segregated the study populations into two geographic groups. Most of the populations in the first geographic group, the coastal region, were resistant to temephos, with three populations (AG, CR and LO) showing RR values > 20 (AG 21.5, CR 23.1, LO 39.4). The populations in the second geographic group, the Amazon jungle and the high jungle, showed moderate levels of resistance, with values ranging between 5.1 (JN) and 7.1 (PU). The exception in this geographic group was the population from PM, which showed a RR value of 28.8 to this insecticide. CONCLUSIONS: The results of this study demonstrate that Ae. aegypti populations in Peru present different resistance intensities to temephos, 3 years after temephos use was discontinued. Resistance to this larvicide should continue to be monitored because it is possible that resistance to temephos could decrease in the absence of routine selection pressures. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-022-05310-x. |
format | Online Article Text |
id | pubmed-9397858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-93978582022-08-24 First national-scale evaluation of temephos resistance in Aedes aegypti in Peru Palomino, Miriam Pinto, Jesus Yañez, Pamela Cornelio, Anali Dias, Luciana Amorim, Quesia Martins, Ademir Jesus Lenhart, Audrey Lima, Jose Bento Pereira Parasit Vectors Research BACKGROUND: The development of resistance against insecticides in Aedes aegypti can lead to operational failures in control programs. Knowledge of the spatial and temporal trends of this resistance is needed to drive effective monitoring campaigns, which in turn provide data on which vector control decision-making should be based. METHODS: Third-stage larvae (L3) from the F1 and F2 generations of 39 Peruvian field populations of Ae. aegypti mosquitoes from established laboratory colonies were evaluated for resistance against the organophosphate insecticide temephos. The 39 populations were originally established from eggs collected in the field with ovitraps in eight departments of Peru during 2018 and 2019. Dose–response bioassays, at 11 concentrations of the insecticide, were performed following WHO recommendations. RESULTS: Of the 39 field populations of Ae. aegypti tested for resistance to temephos , 11 showed high levels of resistance (resistance ratio [RR] > 10), 16 showed moderate levels of resistance (defined as RR values between 5 and 10) and only 12 were susceptible (RR < 5). The results segregated the study populations into two geographic groups. Most of the populations in the first geographic group, the coastal region, were resistant to temephos, with three populations (AG, CR and LO) showing RR values > 20 (AG 21.5, CR 23.1, LO 39.4). The populations in the second geographic group, the Amazon jungle and the high jungle, showed moderate levels of resistance, with values ranging between 5.1 (JN) and 7.1 (PU). The exception in this geographic group was the population from PM, which showed a RR value of 28.8 to this insecticide. CONCLUSIONS: The results of this study demonstrate that Ae. aegypti populations in Peru present different resistance intensities to temephos, 3 years after temephos use was discontinued. Resistance to this larvicide should continue to be monitored because it is possible that resistance to temephos could decrease in the absence of routine selection pressures. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-022-05310-x. BioMed Central 2022-07-11 /pmc/articles/PMC9397858/ /pubmed/35818063 http://dx.doi.org/10.1186/s13071-022-05310-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Palomino, Miriam Pinto, Jesus Yañez, Pamela Cornelio, Anali Dias, Luciana Amorim, Quesia Martins, Ademir Jesus Lenhart, Audrey Lima, Jose Bento Pereira First national-scale evaluation of temephos resistance in Aedes aegypti in Peru |
title | First national-scale evaluation of temephos resistance in Aedes aegypti in Peru |
title_full | First national-scale evaluation of temephos resistance in Aedes aegypti in Peru |
title_fullStr | First national-scale evaluation of temephos resistance in Aedes aegypti in Peru |
title_full_unstemmed | First national-scale evaluation of temephos resistance in Aedes aegypti in Peru |
title_short | First national-scale evaluation of temephos resistance in Aedes aegypti in Peru |
title_sort | first national-scale evaluation of temephos resistance in aedes aegypti in peru |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9397858/ https://www.ncbi.nlm.nih.gov/pubmed/35818063 http://dx.doi.org/10.1186/s13071-022-05310-x |
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