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Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring
There is a disagreement on whether extremely low frequency electromagnetic fields (ELF-EMF) have a beneficial or harmful effect on anxiety-like behavior. Prenatal stress induces frequent disturbances in offspring physiology such as anxiety-like behavior extending to adulthood. This study was designe...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9397925/ https://www.ncbi.nlm.nih.gov/pubmed/35998127 http://dx.doi.org/10.1371/journal.pone.0273206 |
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author | Hosseini, Ehsan Farid Habibi, Mahsa Babri, Shirin Mohaddes, Gisou abkhezr, Hajar Heydari, Hamed |
author_facet | Hosseini, Ehsan Farid Habibi, Mahsa Babri, Shirin Mohaddes, Gisou abkhezr, Hajar Heydari, Hamed |
author_sort | Hosseini, Ehsan |
collection | PubMed |
description | There is a disagreement on whether extremely low frequency electromagnetic fields (ELF-EMF) have a beneficial or harmful effect on anxiety-like behavior. Prenatal stress induces frequent disturbances in offspring physiology such as anxiety-like behavior extending to adulthood. This study was designed to evaluate the effects of prenatal stress and ELF-EMF exposure before and during pregnancy on anxiety-like behavior and some anxiety-related pathways in the hippocampus of female rat offspring. A total of 24 female rats 40 days of age were distributed into four groups of 6 rats each: control, Stress (rats whose mothers underwent chronic stress), EMF (rats whose mothers were exposed to electromagnetic fields) and EMF/S (rats whose mothers were simultaneously exposed to chronic stress and ELF-EMF). The rats were given elevated plus-maze and open field tests and then their brains were dissected and their hippocampus were subjected to analysis. ELISA was used to measure 24(S)-hydroxy cholesterol, corticosterone, and serotonin levels. Cryptochrome2, steroidogenic acute regulatory protein, 3B-Hydroxy steroid dehydrogenase, N-methyl-D-aspartate receptor 2(NMDAr2) and phosphorylated N-methyl-D-aspartate receptor 2(PNMDAr2) were assayed by immunoblotting. Anxiety-like behavior increased in all treatment groups at the same time EMF increased anxiety induced by maternal stress in the EMF/S group. The stress group showed decreased serotonin and increased corticosterone levels. ELF-EMF elevated the PNMDAr2/NMDAr2 ratio and 24(S)-hydroxy cholesterol compared to the control group but did not change corticosterone. EMF did not restore changes induced by stress in behavioral and molecular tests. The results of the current study, clarified that ELF-EMF can induce anxiety-like behavior which may be attributed to an increase in the PNMDAr2/NMDAr2 ratio and 24(S)-OHC in the hippocampus, and prenatal stress may contribute to anxiety via a decrease in serotonin and an increase in corticosterone in the hippocampus. We also found that anxiety-like behavior induced by maternal stress exposure, is exacerbated by electromagnetic fields radiation. |
format | Online Article Text |
id | pubmed-9397925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-93979252022-08-24 Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring Hosseini, Ehsan Farid Habibi, Mahsa Babri, Shirin Mohaddes, Gisou abkhezr, Hajar Heydari, Hamed PLoS One Research Article There is a disagreement on whether extremely low frequency electromagnetic fields (ELF-EMF) have a beneficial or harmful effect on anxiety-like behavior. Prenatal stress induces frequent disturbances in offspring physiology such as anxiety-like behavior extending to adulthood. This study was designed to evaluate the effects of prenatal stress and ELF-EMF exposure before and during pregnancy on anxiety-like behavior and some anxiety-related pathways in the hippocampus of female rat offspring. A total of 24 female rats 40 days of age were distributed into four groups of 6 rats each: control, Stress (rats whose mothers underwent chronic stress), EMF (rats whose mothers were exposed to electromagnetic fields) and EMF/S (rats whose mothers were simultaneously exposed to chronic stress and ELF-EMF). The rats were given elevated plus-maze and open field tests and then their brains were dissected and their hippocampus were subjected to analysis. ELISA was used to measure 24(S)-hydroxy cholesterol, corticosterone, and serotonin levels. Cryptochrome2, steroidogenic acute regulatory protein, 3B-Hydroxy steroid dehydrogenase, N-methyl-D-aspartate receptor 2(NMDAr2) and phosphorylated N-methyl-D-aspartate receptor 2(PNMDAr2) were assayed by immunoblotting. Anxiety-like behavior increased in all treatment groups at the same time EMF increased anxiety induced by maternal stress in the EMF/S group. The stress group showed decreased serotonin and increased corticosterone levels. ELF-EMF elevated the PNMDAr2/NMDAr2 ratio and 24(S)-hydroxy cholesterol compared to the control group but did not change corticosterone. EMF did not restore changes induced by stress in behavioral and molecular tests. The results of the current study, clarified that ELF-EMF can induce anxiety-like behavior which may be attributed to an increase in the PNMDAr2/NMDAr2 ratio and 24(S)-OHC in the hippocampus, and prenatal stress may contribute to anxiety via a decrease in serotonin and an increase in corticosterone in the hippocampus. We also found that anxiety-like behavior induced by maternal stress exposure, is exacerbated by electromagnetic fields radiation. Public Library of Science 2022-08-23 /pmc/articles/PMC9397925/ /pubmed/35998127 http://dx.doi.org/10.1371/journal.pone.0273206 Text en © 2022 Hosseini et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hosseini, Ehsan Farid Habibi, Mahsa Babri, Shirin Mohaddes, Gisou abkhezr, Hajar Heydari, Hamed Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring |
title | Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring |
title_full | Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring |
title_fullStr | Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring |
title_full_unstemmed | Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring |
title_short | Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring |
title_sort | maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9397925/ https://www.ncbi.nlm.nih.gov/pubmed/35998127 http://dx.doi.org/10.1371/journal.pone.0273206 |
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