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Adult health and transition stage-specific rotenone-mediated Drosophila model of Parkinson’s disease: Impact on late-onset neurodegenerative disease models

Parkinson’s disease (PD) affects almost 1% of the population worldwide over the age of 50 years. Exposure to environmental toxins like paraquat and rotenone is a risk factor for sporadic PD which constitutes 95% of total cases. Herbicide rotenone has been shown to cause Parkinsonian symptoms in mult...

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Autores principales: Ayajuddin, Mohamad, Phom, Limamanen, Koza, Zevelou, Modi, Priyanka, Das, Abhik, Chaurasia, Rahul, Thepa, Abuno, Jamir, Nukshimenla, Neikha, Kelevikho, Yenisetti, Sarat Chandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9398202/
https://www.ncbi.nlm.nih.gov/pubmed/36017079
http://dx.doi.org/10.3389/fnmol.2022.896183
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author Ayajuddin, Mohamad
Phom, Limamanen
Koza, Zevelou
Modi, Priyanka
Das, Abhik
Chaurasia, Rahul
Thepa, Abuno
Jamir, Nukshimenla
Neikha, Kelevikho
Yenisetti, Sarat Chandra
author_facet Ayajuddin, Mohamad
Phom, Limamanen
Koza, Zevelou
Modi, Priyanka
Das, Abhik
Chaurasia, Rahul
Thepa, Abuno
Jamir, Nukshimenla
Neikha, Kelevikho
Yenisetti, Sarat Chandra
author_sort Ayajuddin, Mohamad
collection PubMed
description Parkinson’s disease (PD) affects almost 1% of the population worldwide over the age of 50 years. Exposure to environmental toxins like paraquat and rotenone is a risk factor for sporadic PD which constitutes 95% of total cases. Herbicide rotenone has been shown to cause Parkinsonian symptoms in multiple animal models. Drosophila is an excellent model organism for studying neurodegenerative diseases (NDD) including PD. The aging process is characterized by differential expression of genes during different life stages. Hence it is necessary to develop life-stage-matched animal models for late-onset human disease(s) such as PD. Such animal models are critical for understanding the pathophysiology of age-related disease progression and important to understand if a genotropic drug/nutraceutical can be effective during late stages. With this idea, we developed an adult life stage-specific (health and transition phase, during which late-onset NDDs such as PD sets in) rotenone-mediated Drosophila model of idiopathic PD. Drosophila is susceptible to rotenone in dose-time dependent manner. Rotenone-mediated fly model of sporadic PD exhibits mobility defects (independent of mortality), inhibited mitochondrial complex I activity, dopaminergic (DAergic) neuronal dysfunction (no loss of DAergic neuronal number; however, reduction in rate-limiting enzyme tyrosine hydroxylase (TH) synthesis), and alteration in levels of dopamine (DA) and its metabolites; 3,4-Dihydroxyphenylacetic acid (DOPAC) and Homovanilic acid (HVA) in brain-specific fashion. These PD-linked behaviors and brain-specific phenotypes denote the robustness of the present fly model of PD. This novel model will be of great help to decipher life stage-specific genetic targets of small molecule mediated DAergic neuroprotection; understanding of which is critical for formulating therapeutic strategies for PD.
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spelling pubmed-93982022022-08-24 Adult health and transition stage-specific rotenone-mediated Drosophila model of Parkinson’s disease: Impact on late-onset neurodegenerative disease models Ayajuddin, Mohamad Phom, Limamanen Koza, Zevelou Modi, Priyanka Das, Abhik Chaurasia, Rahul Thepa, Abuno Jamir, Nukshimenla Neikha, Kelevikho Yenisetti, Sarat Chandra Front Mol Neurosci Neuroscience Parkinson’s disease (PD) affects almost 1% of the population worldwide over the age of 50 years. Exposure to environmental toxins like paraquat and rotenone is a risk factor for sporadic PD which constitutes 95% of total cases. Herbicide rotenone has been shown to cause Parkinsonian symptoms in multiple animal models. Drosophila is an excellent model organism for studying neurodegenerative diseases (NDD) including PD. The aging process is characterized by differential expression of genes during different life stages. Hence it is necessary to develop life-stage-matched animal models for late-onset human disease(s) such as PD. Such animal models are critical for understanding the pathophysiology of age-related disease progression and important to understand if a genotropic drug/nutraceutical can be effective during late stages. With this idea, we developed an adult life stage-specific (health and transition phase, during which late-onset NDDs such as PD sets in) rotenone-mediated Drosophila model of idiopathic PD. Drosophila is susceptible to rotenone in dose-time dependent manner. Rotenone-mediated fly model of sporadic PD exhibits mobility defects (independent of mortality), inhibited mitochondrial complex I activity, dopaminergic (DAergic) neuronal dysfunction (no loss of DAergic neuronal number; however, reduction in rate-limiting enzyme tyrosine hydroxylase (TH) synthesis), and alteration in levels of dopamine (DA) and its metabolites; 3,4-Dihydroxyphenylacetic acid (DOPAC) and Homovanilic acid (HVA) in brain-specific fashion. These PD-linked behaviors and brain-specific phenotypes denote the robustness of the present fly model of PD. This novel model will be of great help to decipher life stage-specific genetic targets of small molecule mediated DAergic neuroprotection; understanding of which is critical for formulating therapeutic strategies for PD. Frontiers Media S.A. 2022-08-09 /pmc/articles/PMC9398202/ /pubmed/36017079 http://dx.doi.org/10.3389/fnmol.2022.896183 Text en Copyright © 2022 Ayajuddin, Phom, Koza, Modi, Das, Chaurasia, Thepa, Jamir, Neikha and Yenisetti. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Ayajuddin, Mohamad
Phom, Limamanen
Koza, Zevelou
Modi, Priyanka
Das, Abhik
Chaurasia, Rahul
Thepa, Abuno
Jamir, Nukshimenla
Neikha, Kelevikho
Yenisetti, Sarat Chandra
Adult health and transition stage-specific rotenone-mediated Drosophila model of Parkinson’s disease: Impact on late-onset neurodegenerative disease models
title Adult health and transition stage-specific rotenone-mediated Drosophila model of Parkinson’s disease: Impact on late-onset neurodegenerative disease models
title_full Adult health and transition stage-specific rotenone-mediated Drosophila model of Parkinson’s disease: Impact on late-onset neurodegenerative disease models
title_fullStr Adult health and transition stage-specific rotenone-mediated Drosophila model of Parkinson’s disease: Impact on late-onset neurodegenerative disease models
title_full_unstemmed Adult health and transition stage-specific rotenone-mediated Drosophila model of Parkinson’s disease: Impact on late-onset neurodegenerative disease models
title_short Adult health and transition stage-specific rotenone-mediated Drosophila model of Parkinson’s disease: Impact on late-onset neurodegenerative disease models
title_sort adult health and transition stage-specific rotenone-mediated drosophila model of parkinson’s disease: impact on late-onset neurodegenerative disease models
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9398202/
https://www.ncbi.nlm.nih.gov/pubmed/36017079
http://dx.doi.org/10.3389/fnmol.2022.896183
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