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Maternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats
BACKGROUND: Fetal stage is a critical developmental window for the skeletal muscle, but little information is available about the impact of maternal vitamin D (Vit. D) deficiency (VDD) on offspring lean mass development in the adult life of male and female animals. METHODS: Female rats (Wistar Hanno...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9398225/ https://www.ncbi.nlm.nih.gov/pubmed/35582969 http://dx.doi.org/10.1002/jcsm.12986 |
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author | Reis, Natany G. Assis, Ana P. Lautherbach, Natália Gonçalves, Dawit A. Silveira, Wilian A. Morgan, Henrique J.N. Valentim, Rafael R. Almeida, Lucas F. Heck, Lilian C. Zanon, Neusa M. Koike, Tatiana E. Santos, Audrei R. Miyabara, Elen H. Kettelhut, Isis C. Navegantes, Luiz C. |
author_facet | Reis, Natany G. Assis, Ana P. Lautherbach, Natália Gonçalves, Dawit A. Silveira, Wilian A. Morgan, Henrique J.N. Valentim, Rafael R. Almeida, Lucas F. Heck, Lilian C. Zanon, Neusa M. Koike, Tatiana E. Santos, Audrei R. Miyabara, Elen H. Kettelhut, Isis C. Navegantes, Luiz C. |
author_sort | Reis, Natany G. |
collection | PubMed |
description | BACKGROUND: Fetal stage is a critical developmental window for the skeletal muscle, but little information is available about the impact of maternal vitamin D (Vit. D) deficiency (VDD) on offspring lean mass development in the adult life of male and female animals. METHODS: Female rats (Wistar Hannover) were fed either a control (1000 IU Vit. D3/kg) or a VDD diet (0 IU Vit. D3/kg) for 6 weeks and during gestation and lactation. At weaning, male and female offspring were randomly separated and received a standard diet up to 180 days old. RESULTS: Vitamin D deficiency induced muscle atrophy in the male (M‐VDD) offspring at the end of weaning, an effect that was reverted along the time. Following 180 days, fast‐twitch skeletal muscles [extensor digitorum longus (EDL)] from the M‐VDD showed a decrease (20%; P < 0.05) in the number of total fibres but an increase in the cross‐sectional area of IIB (17%; P < 0.05), IIA (19%; P < 0.05) and IIAX (21%; P < 0.05) fibres. The fibre hypertrophy was associated with the higher protein levels of MyoD (73%; P < 0.05) and myogenin (55% %; P < 0.05) and in the number of satellite cells (128.8 ± 14 vs. 91 ± 7.6 nuclei Pax7 + in the M‐CTRL; P < 0.05). M‐VDD increased time to fatigue during ex vivo contractions of EDL muscles and showed an increase in the phosphorylation levels of IGF‐1/insulin receptor and their downstream targets related to anabolic processes and myogenic activation, including Ser (473)Akt and Ser (21/9)GSK‐3β. In such muscles, maternal VDD induced a compensatory increase in the content of calcitriol (two‐fold; P < 0.05) and CYP27B1 (58%; P < 0.05), a metabolizing enzyme that converts calcidiol to calcitriol. Interestingly, most morphological and biochemical changes found in EDL were not observed in slow‐twitch skeletal muscles (soleus) from the M‐VDD group as well as in both EDL and soleus muscles from the female offspring. CONCLUSIONS: These data show that maternal VDD selectively affects the development of type‐II muscle fibres in male offspring rats but not in female offspring rats and suggest that the enhancement of their size and fatigue resistance in fast‐twitch skeletal muscle (EDL) is probably due to a compensatory increase in the muscle content of Vit. D in the adult age. |
format | Online Article Text |
id | pubmed-9398225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93982252022-08-24 Maternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats Reis, Natany G. Assis, Ana P. Lautherbach, Natália Gonçalves, Dawit A. Silveira, Wilian A. Morgan, Henrique J.N. Valentim, Rafael R. Almeida, Lucas F. Heck, Lilian C. Zanon, Neusa M. Koike, Tatiana E. Santos, Audrei R. Miyabara, Elen H. Kettelhut, Isis C. Navegantes, Luiz C. J Cachexia Sarcopenia Muscle Original Articles BACKGROUND: Fetal stage is a critical developmental window for the skeletal muscle, but little information is available about the impact of maternal vitamin D (Vit. D) deficiency (VDD) on offspring lean mass development in the adult life of male and female animals. METHODS: Female rats (Wistar Hannover) were fed either a control (1000 IU Vit. D3/kg) or a VDD diet (0 IU Vit. D3/kg) for 6 weeks and during gestation and lactation. At weaning, male and female offspring were randomly separated and received a standard diet up to 180 days old. RESULTS: Vitamin D deficiency induced muscle atrophy in the male (M‐VDD) offspring at the end of weaning, an effect that was reverted along the time. Following 180 days, fast‐twitch skeletal muscles [extensor digitorum longus (EDL)] from the M‐VDD showed a decrease (20%; P < 0.05) in the number of total fibres but an increase in the cross‐sectional area of IIB (17%; P < 0.05), IIA (19%; P < 0.05) and IIAX (21%; P < 0.05) fibres. The fibre hypertrophy was associated with the higher protein levels of MyoD (73%; P < 0.05) and myogenin (55% %; P < 0.05) and in the number of satellite cells (128.8 ± 14 vs. 91 ± 7.6 nuclei Pax7 + in the M‐CTRL; P < 0.05). M‐VDD increased time to fatigue during ex vivo contractions of EDL muscles and showed an increase in the phosphorylation levels of IGF‐1/insulin receptor and their downstream targets related to anabolic processes and myogenic activation, including Ser (473)Akt and Ser (21/9)GSK‐3β. In such muscles, maternal VDD induced a compensatory increase in the content of calcitriol (two‐fold; P < 0.05) and CYP27B1 (58%; P < 0.05), a metabolizing enzyme that converts calcidiol to calcitriol. Interestingly, most morphological and biochemical changes found in EDL were not observed in slow‐twitch skeletal muscles (soleus) from the M‐VDD group as well as in both EDL and soleus muscles from the female offspring. CONCLUSIONS: These data show that maternal VDD selectively affects the development of type‐II muscle fibres in male offspring rats but not in female offspring rats and suggest that the enhancement of their size and fatigue resistance in fast‐twitch skeletal muscle (EDL) is probably due to a compensatory increase in the muscle content of Vit. D in the adult age. John Wiley and Sons Inc. 2022-05-18 2022-08 /pmc/articles/PMC9398225/ /pubmed/35582969 http://dx.doi.org/10.1002/jcsm.12986 Text en © 2022 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of Society on Sarcopenia, Cachexia and Wasting Disorders. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Reis, Natany G. Assis, Ana P. Lautherbach, Natália Gonçalves, Dawit A. Silveira, Wilian A. Morgan, Henrique J.N. Valentim, Rafael R. Almeida, Lucas F. Heck, Lilian C. Zanon, Neusa M. Koike, Tatiana E. Santos, Audrei R. Miyabara, Elen H. Kettelhut, Isis C. Navegantes, Luiz C. Maternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats |
title | Maternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats |
title_full | Maternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats |
title_fullStr | Maternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats |
title_full_unstemmed | Maternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats |
title_short | Maternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats |
title_sort | maternal vitamin d deficiency affects the morphology and function of glycolytic muscle in adult offspring rats |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9398225/ https://www.ncbi.nlm.nih.gov/pubmed/35582969 http://dx.doi.org/10.1002/jcsm.12986 |
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