Cargando…
Genome‐wide analyses of PAM‐relaxed Cas9 genome editors reveal substantial off‐target effects by ABE8e in rice
PAM‐relaxed Cas9 nucleases, cytosine base editors and adenine base editors are promising tools for precise genome editing in plants. However, their genome‐wide off‐target effects are largely unexplored. Here, we conduct whole‐genome sequencing (WGS) analyses of transgenic plants edited by xCas9, Cas...
Autores principales: | Wu, Yuechao, Ren, Qiurong, Zhong, Zhaohui, Liu, Guanqing, Han, Yangshuo, Bao, Yu, Liu, Li, Xiang, Shuyue, Liu, Shuo, Tang, Xu, Zhou, Jianping, Zheng, Xuelian, Sretenovic, Simon, Zhang, Tao, Qi, Yiping, Zhang, Yong |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9398351/ https://www.ncbi.nlm.nih.gov/pubmed/35524459 http://dx.doi.org/10.1111/pbi.13838 |
Ejemplares similares
-
A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice
por: Tang, Xu, et al.
Publicado: (2018) -
CRISPR‐BETS: a base‐editing design tool for generating stop codons
por: Wu, Yuechao, et al.
Publicado: (2021) -
Improved plant cytosine base editors with high editing activity, purity, and specificity
por: Ren, Qiurong, et al.
Publicado: (2021) -
PhieABEs: a PAM‐less/free high‐efficiency adenine base editor toolbox with wide target scope in plants
por: Tan, Jiantao, et al.
Publicado: (2022) -
Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing
por: Tang, Xu, et al.
Publicado: (2019)