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Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans

Nowadays, human beings are exposed to Cu in varieties of environmental mediums, resulting in health risks needing urgent attention. Our research found that Cu shortened lifespan and induced aging-related phenotypes of Caenorhabditis elegans (C. elegans). Transcriptomics data showed differential expr...

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Autores principales: Zhang, Ying, Zhou, Qian, Lu, Lu, Zhao, Chao, Zhang, Hu, Liu, Ran, Pu, Yuepu, Yin, Lihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9398846/
https://www.ncbi.nlm.nih.gov/pubmed/36017239
http://dx.doi.org/10.1155/2022/5297342
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author Zhang, Ying
Zhou, Qian
Lu, Lu
Zhao, Chao
Zhang, Hu
Liu, Ran
Pu, Yuepu
Yin, Lihong
author_facet Zhang, Ying
Zhou, Qian
Lu, Lu
Zhao, Chao
Zhang, Hu
Liu, Ran
Pu, Yuepu
Yin, Lihong
author_sort Zhang, Ying
collection PubMed
description Nowadays, human beings are exposed to Cu in varieties of environmental mediums, resulting in health risks needing urgent attention. Our research found that Cu shortened lifespan and induced aging-related phenotypes of Caenorhabditis elegans (C. elegans). Transcriptomics data showed differential expression genes induced by Cu were mainly involved in regulation of metabolism and longevity, especially in fatty acid metabolism. Quantitative detection of free fatty acid by GC/MS further found that Cu upregulated free fatty acids of C. elegans. A mechanism study confirmed that Cu promoted the fat accumulation in nematodes, which was owing to disorder of fatty acid desaturase and CoA synthetase, endoplasmic reticulum unfolded protein response (UPR(ER)), mitochondrial membrane potential, and unfolded protein response (UPR(mt)). In addition, Cu activated oxidative stress and prevented DAF-16 translocating into nuclear with a concomitant reduction in the expression of environmental stress-related genes. Taken together, the research suggested that Cu promoted aging and induced fat deposition and oxidative damage.
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spelling pubmed-93988462022-08-24 Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans Zhang, Ying Zhou, Qian Lu, Lu Zhao, Chao Zhang, Hu Liu, Ran Pu, Yuepu Yin, Lihong Oxid Med Cell Longev Research Article Nowadays, human beings are exposed to Cu in varieties of environmental mediums, resulting in health risks needing urgent attention. Our research found that Cu shortened lifespan and induced aging-related phenotypes of Caenorhabditis elegans (C. elegans). Transcriptomics data showed differential expression genes induced by Cu were mainly involved in regulation of metabolism and longevity, especially in fatty acid metabolism. Quantitative detection of free fatty acid by GC/MS further found that Cu upregulated free fatty acids of C. elegans. A mechanism study confirmed that Cu promoted the fat accumulation in nematodes, which was owing to disorder of fatty acid desaturase and CoA synthetase, endoplasmic reticulum unfolded protein response (UPR(ER)), mitochondrial membrane potential, and unfolded protein response (UPR(mt)). In addition, Cu activated oxidative stress and prevented DAF-16 translocating into nuclear with a concomitant reduction in the expression of environmental stress-related genes. Taken together, the research suggested that Cu promoted aging and induced fat deposition and oxidative damage. Hindawi 2022-08-16 /pmc/articles/PMC9398846/ /pubmed/36017239 http://dx.doi.org/10.1155/2022/5297342 Text en Copyright © 2022 Ying Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Ying
Zhou, Qian
Lu, Lu
Zhao, Chao
Zhang, Hu
Liu, Ran
Pu, Yuepu
Yin, Lihong
Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans
title Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans
title_full Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans
title_fullStr Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans
title_full_unstemmed Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans
title_short Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans
title_sort integrating transcriptomics and free fatty acid profiling analysis reveal cu induces shortened lifespan and increased fat accumulation and oxidative damage in c. elegans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9398846/
https://www.ncbi.nlm.nih.gov/pubmed/36017239
http://dx.doi.org/10.1155/2022/5297342
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