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Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen

The P. longifolia mediated silver (PL-AgNPs) nanoparticles are very stable and efficient. UV–Vis spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy dispersive X-ray spectroscopy (EDX) were used...

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Autores principales: Dashora, Alankrita, Rathore, Kavita, Raj, Shani, Sharma, Kanika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9398913/
https://www.ncbi.nlm.nih.gov/pubmed/36032398
http://dx.doi.org/10.1016/j.bbrep.2022.101320
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author Dashora, Alankrita
Rathore, Kavita
Raj, Shani
Sharma, Kanika
author_facet Dashora, Alankrita
Rathore, Kavita
Raj, Shani
Sharma, Kanika
author_sort Dashora, Alankrita
collection PubMed
description The P. longifolia mediated silver (PL-AgNPs) nanoparticles are very stable and efficient. UV–Vis spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy dispersive X-ray spectroscopy (EDX) were used to characterize the produced AgNPs. UV–Vis analysis showed a characteristic peak at 435 nm corresponding to surface plasmon resonance. The synthesis process was spectrophotometrically optimized for various parameters. After optimization, highly stable AgNPs were prepared using 3.0 ml of P. longifolia leaf extract, pH 7.0, 1.0 mM AgNO(3), and 60 °C. The zeta potential was measured by DLS, which showed −20.8 mV and the PDI value was 5.42. TEM and SEM analysis shows a spherical shape of the synthesized nanoparticles, and the size was measured between 10 and 40 nm. EDX analysis showed intense peaks from silver and oxygen and small peaks from various metal atoms such as Na, P, S and Al indicating their presence in trace amounts. The average size of the PL-AgNPs was 14 nm. The phytochemical analysis shows that the presence of alkaloids, essential oils and saponins seems to be responsible for the synthesis of nanoparticles. PL-AgNPs were further investigated for their antifungal activity against Alternaria alternata. The minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and effect of nanoparticles on cytomorphology of A. alternata have also been reported. Biosynthesized nanoparticles have proven to be inexpensive, environmentally friendly, stable, easily reproducible, and highly effective against plant-pathogenic fungi.
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spelling pubmed-93989132022-08-25 Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen Dashora, Alankrita Rathore, Kavita Raj, Shani Sharma, Kanika Biochem Biophys Rep Research Article The P. longifolia mediated silver (PL-AgNPs) nanoparticles are very stable and efficient. UV–Vis spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy dispersive X-ray spectroscopy (EDX) were used to characterize the produced AgNPs. UV–Vis analysis showed a characteristic peak at 435 nm corresponding to surface plasmon resonance. The synthesis process was spectrophotometrically optimized for various parameters. After optimization, highly stable AgNPs were prepared using 3.0 ml of P. longifolia leaf extract, pH 7.0, 1.0 mM AgNO(3), and 60 °C. The zeta potential was measured by DLS, which showed −20.8 mV and the PDI value was 5.42. TEM and SEM analysis shows a spherical shape of the synthesized nanoparticles, and the size was measured between 10 and 40 nm. EDX analysis showed intense peaks from silver and oxygen and small peaks from various metal atoms such as Na, P, S and Al indicating their presence in trace amounts. The average size of the PL-AgNPs was 14 nm. The phytochemical analysis shows that the presence of alkaloids, essential oils and saponins seems to be responsible for the synthesis of nanoparticles. PL-AgNPs were further investigated for their antifungal activity against Alternaria alternata. The minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and effect of nanoparticles on cytomorphology of A. alternata have also been reported. Biosynthesized nanoparticles have proven to be inexpensive, environmentally friendly, stable, easily reproducible, and highly effective against plant-pathogenic fungi. Elsevier 2022-08-12 /pmc/articles/PMC9398913/ /pubmed/36032398 http://dx.doi.org/10.1016/j.bbrep.2022.101320 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Dashora, Alankrita
Rathore, Kavita
Raj, Shani
Sharma, Kanika
Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen
title Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen
title_full Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen
title_fullStr Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen
title_full_unstemmed Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen
title_short Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen
title_sort synthesis of silver nanoparticles employing polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9398913/
https://www.ncbi.nlm.nih.gov/pubmed/36032398
http://dx.doi.org/10.1016/j.bbrep.2022.101320
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