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Nanoscale 3D spatial addressing and valence control of quantum dots using wireframe DNA origami

Control over the copy number and nanoscale positioning of quantum dots (QDs) is critical to their application to functional nanomaterials design. However, the multiple non-specific binding sites intrinsic to the surface of QDs have prevented their fabrication into multi-QD assemblies with programmed...

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Autores principales: Chen, Chi, Wei, Xingfei, Parsons, Molly F., Guo, Jiajia, Banal, James L., Zhao, Yinong, Scott, Madelyn N., Schlau-Cohen, Gabriela S., Hernandez, Rigoberto, Bathe, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399249/
https://www.ncbi.nlm.nih.gov/pubmed/35999227
http://dx.doi.org/10.1038/s41467-022-32662-w
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author Chen, Chi
Wei, Xingfei
Parsons, Molly F.
Guo, Jiajia
Banal, James L.
Zhao, Yinong
Scott, Madelyn N.
Schlau-Cohen, Gabriela S.
Hernandez, Rigoberto
Bathe, Mark
author_facet Chen, Chi
Wei, Xingfei
Parsons, Molly F.
Guo, Jiajia
Banal, James L.
Zhao, Yinong
Scott, Madelyn N.
Schlau-Cohen, Gabriela S.
Hernandez, Rigoberto
Bathe, Mark
author_sort Chen, Chi
collection PubMed
description Control over the copy number and nanoscale positioning of quantum dots (QDs) is critical to their application to functional nanomaterials design. However, the multiple non-specific binding sites intrinsic to the surface of QDs have prevented their fabrication into multi-QD assemblies with programmed spatial positions. To overcome this challenge, we developed a general synthetic framework to selectively attach spatially addressable QDs on 3D wireframe DNA origami scaffolds using interfacial control of the QD surface. Using optical spectroscopy and molecular dynamics simulation, we investigated the fabrication of monovalent QDs of different sizes using chimeric single-stranded DNA to control QD surface chemistry. By understanding the relationship between chimeric single-stranded DNA length and QD size, we integrated single QDs into wireframe DNA origami objects and visualized the resulting QD-DNA assemblies using electron microscopy. Using these advances, we demonstrated the ability to program arbitrary 3D spatial relationships between QDs and dyes on DNA origami objects by fabricating energy-transfer circuits and colloidal molecules. Our design and fabrication approach enables the geometric control and spatial addressing of QDs together with the integration of other materials including dyes to fabricate hybrid materials for functional nanoscale photonic devices.
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spelling pubmed-93992492022-08-25 Nanoscale 3D spatial addressing and valence control of quantum dots using wireframe DNA origami Chen, Chi Wei, Xingfei Parsons, Molly F. Guo, Jiajia Banal, James L. Zhao, Yinong Scott, Madelyn N. Schlau-Cohen, Gabriela S. Hernandez, Rigoberto Bathe, Mark Nat Commun Article Control over the copy number and nanoscale positioning of quantum dots (QDs) is critical to their application to functional nanomaterials design. However, the multiple non-specific binding sites intrinsic to the surface of QDs have prevented their fabrication into multi-QD assemblies with programmed spatial positions. To overcome this challenge, we developed a general synthetic framework to selectively attach spatially addressable QDs on 3D wireframe DNA origami scaffolds using interfacial control of the QD surface. Using optical spectroscopy and molecular dynamics simulation, we investigated the fabrication of monovalent QDs of different sizes using chimeric single-stranded DNA to control QD surface chemistry. By understanding the relationship between chimeric single-stranded DNA length and QD size, we integrated single QDs into wireframe DNA origami objects and visualized the resulting QD-DNA assemblies using electron microscopy. Using these advances, we demonstrated the ability to program arbitrary 3D spatial relationships between QDs and dyes on DNA origami objects by fabricating energy-transfer circuits and colloidal molecules. Our design and fabrication approach enables the geometric control and spatial addressing of QDs together with the integration of other materials including dyes to fabricate hybrid materials for functional nanoscale photonic devices. Nature Publishing Group UK 2022-08-23 /pmc/articles/PMC9399249/ /pubmed/35999227 http://dx.doi.org/10.1038/s41467-022-32662-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Chen, Chi
Wei, Xingfei
Parsons, Molly F.
Guo, Jiajia
Banal, James L.
Zhao, Yinong
Scott, Madelyn N.
Schlau-Cohen, Gabriela S.
Hernandez, Rigoberto
Bathe, Mark
Nanoscale 3D spatial addressing and valence control of quantum dots using wireframe DNA origami
title Nanoscale 3D spatial addressing and valence control of quantum dots using wireframe DNA origami
title_full Nanoscale 3D spatial addressing and valence control of quantum dots using wireframe DNA origami
title_fullStr Nanoscale 3D spatial addressing and valence control of quantum dots using wireframe DNA origami
title_full_unstemmed Nanoscale 3D spatial addressing and valence control of quantum dots using wireframe DNA origami
title_short Nanoscale 3D spatial addressing and valence control of quantum dots using wireframe DNA origami
title_sort nanoscale 3d spatial addressing and valence control of quantum dots using wireframe dna origami
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399249/
https://www.ncbi.nlm.nih.gov/pubmed/35999227
http://dx.doi.org/10.1038/s41467-022-32662-w
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