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Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia
Two zoonotic protozoan pathogens, Giardia duodenalis and Toxoplasma gondii, are important causes of waterborne infections in the Quindío region in Colombia. No previous data exist on how contamination occurs at the source for drinking water consumed by the human population in this region. Our aim wa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399255/ https://www.ncbi.nlm.nih.gov/pubmed/36035634 http://dx.doi.org/10.1016/j.fawpar.2022.e00175 |
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author | Pinto-Duarte, Valeria Alejandra Hérnandez-Arango, Natalia Marcela Marin-Gallego, Benyi Juliana Toloza-Beltrán, Paola Andrea Lora-Suarez, Fabiana María Gómez-Marín, Jorge Enrique |
author_facet | Pinto-Duarte, Valeria Alejandra Hérnandez-Arango, Natalia Marcela Marin-Gallego, Benyi Juliana Toloza-Beltrán, Paola Andrea Lora-Suarez, Fabiana María Gómez-Marín, Jorge Enrique |
author_sort | Pinto-Duarte, Valeria Alejandra |
collection | PubMed |
description | Two zoonotic protozoan pathogens, Giardia duodenalis and Toxoplasma gondii, are important causes of waterborne infections in the Quindío region in Colombia. No previous data exist on how contamination occurs at the source for drinking water consumed by the human population in this region. Our aim was to describe the frequency of G. duodenalis and T. gondii DNA in 11 sampling points during a five-month period in water and adjacent soil at the Quindío River basin (Andean region in the central western part of Colombia). The study employed nested PCR for T. gondii, using the B1 gene as the amplification target, and single-round PCR for G. duodenalis assemblage A and assemblage B, amplifying the gdh gene, followed by DNA sequencing. In 50 soil samples, 28% (14/50) were positive for T. gondii. For G. duodenalis, distribution was in equal parts for assemblage A (8%; 4/50) and assemblage B (8%, 4/50). Genotyping of T. gondii sequences showed two soil samples with type I strain, another two samples of soil with type III strain, but most samples were of unidentified strains. In water samples, T. gondii was detected in 9.1% (5/55), G. duodenalis assemblage A in 34.5% (19/55), and G. duodenalis assemblage B in 12.7% (7/55). T. gondii DNA positivity was associated with lower soil temperature (p = 0.0239). Presence of G. duodenalis and T. gondii was evidenced in soil and water samples in the Quindío River basin, indicating soil as the potential source of contamination for the river that it is destined for human consumption. Monitoring these protozoa in drinking water is necessary to prevent public health risks in human populations. |
format | Online Article Text |
id | pubmed-9399255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-93992552022-08-25 Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia Pinto-Duarte, Valeria Alejandra Hérnandez-Arango, Natalia Marcela Marin-Gallego, Benyi Juliana Toloza-Beltrán, Paola Andrea Lora-Suarez, Fabiana María Gómez-Marín, Jorge Enrique Food Waterborne Parasitol Research Article Two zoonotic protozoan pathogens, Giardia duodenalis and Toxoplasma gondii, are important causes of waterborne infections in the Quindío region in Colombia. No previous data exist on how contamination occurs at the source for drinking water consumed by the human population in this region. Our aim was to describe the frequency of G. duodenalis and T. gondii DNA in 11 sampling points during a five-month period in water and adjacent soil at the Quindío River basin (Andean region in the central western part of Colombia). The study employed nested PCR for T. gondii, using the B1 gene as the amplification target, and single-round PCR for G. duodenalis assemblage A and assemblage B, amplifying the gdh gene, followed by DNA sequencing. In 50 soil samples, 28% (14/50) were positive for T. gondii. For G. duodenalis, distribution was in equal parts for assemblage A (8%; 4/50) and assemblage B (8%, 4/50). Genotyping of T. gondii sequences showed two soil samples with type I strain, another two samples of soil with type III strain, but most samples were of unidentified strains. In water samples, T. gondii was detected in 9.1% (5/55), G. duodenalis assemblage A in 34.5% (19/55), and G. duodenalis assemblage B in 12.7% (7/55). T. gondii DNA positivity was associated with lower soil temperature (p = 0.0239). Presence of G. duodenalis and T. gondii was evidenced in soil and water samples in the Quindío River basin, indicating soil as the potential source of contamination for the river that it is destined for human consumption. Monitoring these protozoa in drinking water is necessary to prevent public health risks in human populations. Elsevier 2022-08-09 /pmc/articles/PMC9399255/ /pubmed/36035634 http://dx.doi.org/10.1016/j.fawpar.2022.e00175 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Pinto-Duarte, Valeria Alejandra Hérnandez-Arango, Natalia Marcela Marin-Gallego, Benyi Juliana Toloza-Beltrán, Paola Andrea Lora-Suarez, Fabiana María Gómez-Marín, Jorge Enrique Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
title | Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
title_full | Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
title_fullStr | Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
title_full_unstemmed | Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
title_short | Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
title_sort | detection of giardia duodenalis and toxoplasma gondii in soil and water samples in the quindío river basin, colombia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399255/ https://www.ncbi.nlm.nih.gov/pubmed/36035634 http://dx.doi.org/10.1016/j.fawpar.2022.e00175 |
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