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High percentages of peripheral blood T-cell activation in childhood Hodgkin's lymphoma are associated with inferior outcome
The aims of this study were to investigate the activation of T lymphocytes in peripheral blood from children with Hodgkin's lymphoma (HL) and explore their roles for prognosis in HL. A cohort of 52 newly diagnosed children with HL during the past 10 years was enrolled for analysis in this study...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399494/ https://www.ncbi.nlm.nih.gov/pubmed/36035394 http://dx.doi.org/10.3389/fmed.2022.955373 |
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author | Cai, Fengqing Gao, Hui Yu, Zhongsheng Zhu, Kun Gu, Weizhong Guo, Xiaoping Xu, Xiaojun Shen, Hongqiang Shu, Qiang |
author_facet | Cai, Fengqing Gao, Hui Yu, Zhongsheng Zhu, Kun Gu, Weizhong Guo, Xiaoping Xu, Xiaojun Shen, Hongqiang Shu, Qiang |
author_sort | Cai, Fengqing |
collection | PubMed |
description | The aims of this study were to investigate the activation of T lymphocytes in peripheral blood from children with Hodgkin's lymphoma (HL) and explore their roles for prognosis in HL. A cohort of 52 newly diagnosed children with HL during the past 10 years was enrolled for analysis in this study. Peripheral blood samples of the patients were acquired before treatment in our hospital, and T-cell subsets were detected by a four-color flow cytometer. CD4+ T cells and CD4+/CD8+ T-cell ratio decreased significantly in patients with HL vs. healthy controls. CD8+ T cells, CD3+CD4+HLA-DR+ T cells, and CD3+CD8+HLA-DR+ T cells increased markedly in patients with HL vs. healthy controls. Receiver-operating characteristic (ROC) curve analysis showed that CD3+CD4+HLA-DR+ T cells and CD3+CD8+HLA-DR+ T cells each distinguished the high-risk group from the low- and intermediate-risk group. The area under the ROC curve for predicting high-risk patients was 0.795 for CD3+CD4+HLA-DR+ T cell and 0.784 for CD3+CD8+HLA-DR+ T cell. A comparison of peripheral blood T-cell subsets that responded differently to therapy showed significantly higher percentages of CD3+CD4+HLA-DR+ T cells and CD3+CD8+HLA-DR+ T cells in patients who achieved complete remission compared to those who did not achieve complete remission. In addition, high percentages of both CD3+CD4+HLA-DR+ T cells and CD3+CD8+HLA-DR+ T cells were associated with inferior event-free survival. Peripheral immune status may be related to disease severity in HL. CD3+CD4+HLA-DR+ T cells and CD3+CD8+HLA-DR+ T cells may be a novel indicator for risk stratification of HL and may be an independent risk factor for inferior outcome in childhood HL. |
format | Online Article Text |
id | pubmed-9399494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93994942022-08-25 High percentages of peripheral blood T-cell activation in childhood Hodgkin's lymphoma are associated with inferior outcome Cai, Fengqing Gao, Hui Yu, Zhongsheng Zhu, Kun Gu, Weizhong Guo, Xiaoping Xu, Xiaojun Shen, Hongqiang Shu, Qiang Front Med (Lausanne) Medicine The aims of this study were to investigate the activation of T lymphocytes in peripheral blood from children with Hodgkin's lymphoma (HL) and explore their roles for prognosis in HL. A cohort of 52 newly diagnosed children with HL during the past 10 years was enrolled for analysis in this study. Peripheral blood samples of the patients were acquired before treatment in our hospital, and T-cell subsets were detected by a four-color flow cytometer. CD4+ T cells and CD4+/CD8+ T-cell ratio decreased significantly in patients with HL vs. healthy controls. CD8+ T cells, CD3+CD4+HLA-DR+ T cells, and CD3+CD8+HLA-DR+ T cells increased markedly in patients with HL vs. healthy controls. Receiver-operating characteristic (ROC) curve analysis showed that CD3+CD4+HLA-DR+ T cells and CD3+CD8+HLA-DR+ T cells each distinguished the high-risk group from the low- and intermediate-risk group. The area under the ROC curve for predicting high-risk patients was 0.795 for CD3+CD4+HLA-DR+ T cell and 0.784 for CD3+CD8+HLA-DR+ T cell. A comparison of peripheral blood T-cell subsets that responded differently to therapy showed significantly higher percentages of CD3+CD4+HLA-DR+ T cells and CD3+CD8+HLA-DR+ T cells in patients who achieved complete remission compared to those who did not achieve complete remission. In addition, high percentages of both CD3+CD4+HLA-DR+ T cells and CD3+CD8+HLA-DR+ T cells were associated with inferior event-free survival. Peripheral immune status may be related to disease severity in HL. CD3+CD4+HLA-DR+ T cells and CD3+CD8+HLA-DR+ T cells may be a novel indicator for risk stratification of HL and may be an independent risk factor for inferior outcome in childhood HL. Frontiers Media S.A. 2022-08-10 /pmc/articles/PMC9399494/ /pubmed/36035394 http://dx.doi.org/10.3389/fmed.2022.955373 Text en Copyright © 2022 Cai, Gao, Yu, Zhu, Gu, Guo, Xu, Shen and Shu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Cai, Fengqing Gao, Hui Yu, Zhongsheng Zhu, Kun Gu, Weizhong Guo, Xiaoping Xu, Xiaojun Shen, Hongqiang Shu, Qiang High percentages of peripheral blood T-cell activation in childhood Hodgkin's lymphoma are associated with inferior outcome |
title | High percentages of peripheral blood T-cell activation in childhood Hodgkin's lymphoma are associated with inferior outcome |
title_full | High percentages of peripheral blood T-cell activation in childhood Hodgkin's lymphoma are associated with inferior outcome |
title_fullStr | High percentages of peripheral blood T-cell activation in childhood Hodgkin's lymphoma are associated with inferior outcome |
title_full_unstemmed | High percentages of peripheral blood T-cell activation in childhood Hodgkin's lymphoma are associated with inferior outcome |
title_short | High percentages of peripheral blood T-cell activation in childhood Hodgkin's lymphoma are associated with inferior outcome |
title_sort | high percentages of peripheral blood t-cell activation in childhood hodgkin's lymphoma are associated with inferior outcome |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399494/ https://www.ncbi.nlm.nih.gov/pubmed/36035394 http://dx.doi.org/10.3389/fmed.2022.955373 |
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