Cargando…
Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings
Kasugamycin (KSM), an aminoglycoside antibiotic, has been widely used for the management of plant diseases, especially for the control of rice blast in Asia. However, its uptake mechanism and transport in plants are still obscure. The castor bean (Ricinus communis L.) seeding, a model plant for phlo...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399671/ https://www.ncbi.nlm.nih.gov/pubmed/36033898 http://dx.doi.org/10.3389/fmicb.2022.948171 |
_version_ | 1784772578213101568 |
---|---|
author | Zhang, Hongzhen Zhang, Chenghua Xiang, Xiaolong Zhang, Qilun Zhao, Wei Wei, Guoyu Hu, Anlong |
author_facet | Zhang, Hongzhen Zhang, Chenghua Xiang, Xiaolong Zhang, Qilun Zhao, Wei Wei, Guoyu Hu, Anlong |
author_sort | Zhang, Hongzhen |
collection | PubMed |
description | Kasugamycin (KSM), an aminoglycoside antibiotic, has been widely used for the management of plant diseases, especially for the control of rice blast in Asia. However, its uptake mechanism and transport in plants are still obscure. The castor bean (Ricinus communis L.) seeding, a model plant for phloem transport, was used to study the mechanism of uptake and transport of KSM. Results showed that cotyledon-applied KSM could transport into the phloem and distributed in root and shoot of plant. The temperature, concentration, and pH had significant effects on the uptake of KSM, indicating that the uptake of KSM was mediated by an active carrier system. Compared with the control, competitive inhibitors of sugar transporters D-glucose, D-chiro-inositol, and phloridzin inhibited 71.03%, 67.95%, and 61.73% uptake of KSM, respectively. Energy inhibitor dinitrophenol (DNP) and carbonyl cyanide chlorophenylhydrazone (CCCP) also affected the uptake of KSM, and the inhibition rates were 34.23% and 48.06%. All the results showed that the uptake of KSM was mediated by a sugar transporter, and it could transport from shoot to root in plants via the phloem. The study preliminary elucidated the plant–microbe interactions in the context of the transport of microbial secondary metabolites in plants. It has certain significance for scientific application of antibiotics and biological control of plant diseases and provides theoretical basis for the development of bidirectional transport pesticides. |
format | Online Article Text |
id | pubmed-9399671 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93996712022-08-25 Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings Zhang, Hongzhen Zhang, Chenghua Xiang, Xiaolong Zhang, Qilun Zhao, Wei Wei, Guoyu Hu, Anlong Front Microbiol Microbiology Kasugamycin (KSM), an aminoglycoside antibiotic, has been widely used for the management of plant diseases, especially for the control of rice blast in Asia. However, its uptake mechanism and transport in plants are still obscure. The castor bean (Ricinus communis L.) seeding, a model plant for phloem transport, was used to study the mechanism of uptake and transport of KSM. Results showed that cotyledon-applied KSM could transport into the phloem and distributed in root and shoot of plant. The temperature, concentration, and pH had significant effects on the uptake of KSM, indicating that the uptake of KSM was mediated by an active carrier system. Compared with the control, competitive inhibitors of sugar transporters D-glucose, D-chiro-inositol, and phloridzin inhibited 71.03%, 67.95%, and 61.73% uptake of KSM, respectively. Energy inhibitor dinitrophenol (DNP) and carbonyl cyanide chlorophenylhydrazone (CCCP) also affected the uptake of KSM, and the inhibition rates were 34.23% and 48.06%. All the results showed that the uptake of KSM was mediated by a sugar transporter, and it could transport from shoot to root in plants via the phloem. The study preliminary elucidated the plant–microbe interactions in the context of the transport of microbial secondary metabolites in plants. It has certain significance for scientific application of antibiotics and biological control of plant diseases and provides theoretical basis for the development of bidirectional transport pesticides. Frontiers Media S.A. 2022-08-10 /pmc/articles/PMC9399671/ /pubmed/36033898 http://dx.doi.org/10.3389/fmicb.2022.948171 Text en Copyright © 2022 Zhang, Zhang, Xiang, Zhang, Zhao, Wei and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhang, Hongzhen Zhang, Chenghua Xiang, Xiaolong Zhang, Qilun Zhao, Wei Wei, Guoyu Hu, Anlong Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
title | Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
title_full | Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
title_fullStr | Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
title_full_unstemmed | Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
title_short | Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
title_sort | uptake and transport of antibiotic kasugamycin in castor bean (ricinus communis l.) seedlings |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399671/ https://www.ncbi.nlm.nih.gov/pubmed/36033898 http://dx.doi.org/10.3389/fmicb.2022.948171 |
work_keys_str_mv | AT zhanghongzhen uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT zhangchenghua uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT xiangxiaolong uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT zhangqilun uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT zhaowei uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT weiguoyu uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT huanlong uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings |