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Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs)

Polydopamine (PDA) is a biocompatible cell-adhesive polymer with versatile applications in biomedical devices. Previous studies have shown that PDA coating could improve cell adhesion and differentiation of human mesenchymal stem cells (hMSCs). However, there is still a knowledge gap in the effect o...

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Autores principales: Harati, Javad, Tao, Xuelian, Shahsavarani, Hosein, Du, Ping, Galluzzi, Massimiliano, Liu, Kun, Zhang, Zhen, Shaw, Peter, Shokrgozar, Mohammad Ali, Pan, Haobo, Wang, Peng-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399727/
https://www.ncbi.nlm.nih.gov/pubmed/36032703
http://dx.doi.org/10.3389/fbioe.2022.934179
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author Harati, Javad
Tao, Xuelian
Shahsavarani, Hosein
Du, Ping
Galluzzi, Massimiliano
Liu, Kun
Zhang, Zhen
Shaw, Peter
Shokrgozar, Mohammad Ali
Pan, Haobo
Wang, Peng-Yuan
author_facet Harati, Javad
Tao, Xuelian
Shahsavarani, Hosein
Du, Ping
Galluzzi, Massimiliano
Liu, Kun
Zhang, Zhen
Shaw, Peter
Shokrgozar, Mohammad Ali
Pan, Haobo
Wang, Peng-Yuan
author_sort Harati, Javad
collection PubMed
description Polydopamine (PDA) is a biocompatible cell-adhesive polymer with versatile applications in biomedical devices. Previous studies have shown that PDA coating could improve cell adhesion and differentiation of human mesenchymal stem cells (hMSCs). However, there is still a knowledge gap in the effect of PDA-mediated protein adsorption on the epigenetic status of MSCs. This work used gelatin-coated cell culture surfaces with and without PDA underlayer (Gel and PDA-Gel) to culture and differentiate primary human adipose-derived stem cells (hASCs). The properties of these two substrates were significantly different, which, in combination with a variation in extracellular matrix (ECM) protein bioactivity, regulated cell adhesion and migration. hASCs reduced focal adhesions by downregulating the expression of integrins such as αV, α1, α2, and β1 on the PDA-Gel compared to the Gel substrate. Interestingly, the ratio of H3K27me3 to H3K27me3+H3K4me3 was decreased, but this only occurred for upregulation of AGG and BMP4 genes during chondrogenic differentiation. This result implies that the PDA-Gel surface positively affects the chondrogenic, but not adipogenic and osteogenic, differentiation. In conclusion, for the first time, this study demonstrates the sequential effects of PDA coating on the biophysical property of adsorbed protein and then focal adhesions and differentiation of hMSCs through epigenetic regulation. This study sheds light on PDA-mediated mechanotransduction.
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spelling pubmed-93997272022-08-25 Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs) Harati, Javad Tao, Xuelian Shahsavarani, Hosein Du, Ping Galluzzi, Massimiliano Liu, Kun Zhang, Zhen Shaw, Peter Shokrgozar, Mohammad Ali Pan, Haobo Wang, Peng-Yuan Front Bioeng Biotechnol Bioengineering and Biotechnology Polydopamine (PDA) is a biocompatible cell-adhesive polymer with versatile applications in biomedical devices. Previous studies have shown that PDA coating could improve cell adhesion and differentiation of human mesenchymal stem cells (hMSCs). However, there is still a knowledge gap in the effect of PDA-mediated protein adsorption on the epigenetic status of MSCs. This work used gelatin-coated cell culture surfaces with and without PDA underlayer (Gel and PDA-Gel) to culture and differentiate primary human adipose-derived stem cells (hASCs). The properties of these two substrates were significantly different, which, in combination with a variation in extracellular matrix (ECM) protein bioactivity, regulated cell adhesion and migration. hASCs reduced focal adhesions by downregulating the expression of integrins such as αV, α1, α2, and β1 on the PDA-Gel compared to the Gel substrate. Interestingly, the ratio of H3K27me3 to H3K27me3+H3K4me3 was decreased, but this only occurred for upregulation of AGG and BMP4 genes during chondrogenic differentiation. This result implies that the PDA-Gel surface positively affects the chondrogenic, but not adipogenic and osteogenic, differentiation. In conclusion, for the first time, this study demonstrates the sequential effects of PDA coating on the biophysical property of adsorbed protein and then focal adhesions and differentiation of hMSCs through epigenetic regulation. This study sheds light on PDA-mediated mechanotransduction. Frontiers Media S.A. 2022-08-10 /pmc/articles/PMC9399727/ /pubmed/36032703 http://dx.doi.org/10.3389/fbioe.2022.934179 Text en Copyright © 2022 Harati, Tao, Shahsavarani, Du, Galluzzi, Liu, Zhang, Shaw, Shokrgozar, Pan and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Harati, Javad
Tao, Xuelian
Shahsavarani, Hosein
Du, Ping
Galluzzi, Massimiliano
Liu, Kun
Zhang, Zhen
Shaw, Peter
Shokrgozar, Mohammad Ali
Pan, Haobo
Wang, Peng-Yuan
Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs)
title Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs)
title_full Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs)
title_fullStr Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs)
title_full_unstemmed Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs)
title_short Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs)
title_sort polydopamine-mediated protein adsorption alters the epigenetic status and differentiation of primary human adipose-derived stem cells (hascs)
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399727/
https://www.ncbi.nlm.nih.gov/pubmed/36032703
http://dx.doi.org/10.3389/fbioe.2022.934179
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