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Prenatal antibiotics exposure does not influence experimental allergic asthma in mice
Changes in microbiome (dysbiosis) contribute to severity of allergic asthma. Preexisting epidemiological studies in humans correlate perinatal dysbiosis with increased long-term asthma severity. However, these studies cannot discriminate between prenatal and postnatal effects of dysbiosis and suffer...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399857/ https://www.ncbi.nlm.nih.gov/pubmed/36032166 http://dx.doi.org/10.3389/fimmu.2022.937577 |
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author | Lingel, Imke Wilburn, Adrienne N. Hargis, Julie McAlees, Jaclyn W. Laumonnier, Yves Chougnet, Claire A. Deshmukh, Hitesh König, Peter Lewkowich, Ian P. Schmudde, Inken |
author_facet | Lingel, Imke Wilburn, Adrienne N. Hargis, Julie McAlees, Jaclyn W. Laumonnier, Yves Chougnet, Claire A. Deshmukh, Hitesh König, Peter Lewkowich, Ian P. Schmudde, Inken |
author_sort | Lingel, Imke |
collection | PubMed |
description | Changes in microbiome (dysbiosis) contribute to severity of allergic asthma. Preexisting epidemiological studies in humans correlate perinatal dysbiosis with increased long-term asthma severity. However, these studies cannot discriminate between prenatal and postnatal effects of dysbiosis and suffer from a high variability of dysbiotic causes ranging from antibiotic treatment, delivery by caesarian section to early-life breastfeeding practices. Given that maternal antibiotic exposure in mice increases the risk of newborn bacterial pneumonia in offspring, we hypothesized that prenatal maternal antibiotic-induced dysbiosis induces long-term immunological effects in the offspring that also increase long-term asthma severity. Therefore, dams were exposed to antibiotics (gentamycin, ampicillin, vancomycin) from embryonic day 15 until birth. Six weeks later, asthma was induced in the offspring by repeated applications of house dust mite extract. Airway function, cytokine production, pulmonary cell composition and distribution were assessed. Our study revealed that prenatally induced dysbiosis in mice led to an increase in pulmonary Th17(+) non-conventional T cells with limited functional effect on airway resistance, pro-asthmatic Th2/Th17 cytokine production, pulmonary localization and cell-cell contacts. These data indicate that dysbiosis-related immune-modulation with long-term effects on asthma development occurs to a lesser extent prenatally and will allow to focus future studies on more decisive postnatal timeframes. |
format | Online Article Text |
id | pubmed-9399857 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93998572022-08-25 Prenatal antibiotics exposure does not influence experimental allergic asthma in mice Lingel, Imke Wilburn, Adrienne N. Hargis, Julie McAlees, Jaclyn W. Laumonnier, Yves Chougnet, Claire A. Deshmukh, Hitesh König, Peter Lewkowich, Ian P. Schmudde, Inken Front Immunol Immunology Changes in microbiome (dysbiosis) contribute to severity of allergic asthma. Preexisting epidemiological studies in humans correlate perinatal dysbiosis with increased long-term asthma severity. However, these studies cannot discriminate between prenatal and postnatal effects of dysbiosis and suffer from a high variability of dysbiotic causes ranging from antibiotic treatment, delivery by caesarian section to early-life breastfeeding practices. Given that maternal antibiotic exposure in mice increases the risk of newborn bacterial pneumonia in offspring, we hypothesized that prenatal maternal antibiotic-induced dysbiosis induces long-term immunological effects in the offspring that also increase long-term asthma severity. Therefore, dams were exposed to antibiotics (gentamycin, ampicillin, vancomycin) from embryonic day 15 until birth. Six weeks later, asthma was induced in the offspring by repeated applications of house dust mite extract. Airway function, cytokine production, pulmonary cell composition and distribution were assessed. Our study revealed that prenatally induced dysbiosis in mice led to an increase in pulmonary Th17(+) non-conventional T cells with limited functional effect on airway resistance, pro-asthmatic Th2/Th17 cytokine production, pulmonary localization and cell-cell contacts. These data indicate that dysbiosis-related immune-modulation with long-term effects on asthma development occurs to a lesser extent prenatally and will allow to focus future studies on more decisive postnatal timeframes. Frontiers Media S.A. 2022-08-10 /pmc/articles/PMC9399857/ /pubmed/36032166 http://dx.doi.org/10.3389/fimmu.2022.937577 Text en Copyright © 2022 Lingel, Wilburn, Hargis, McAlees, Laumonnier, Chougnet, Deshmukh, König, Lewkowich and Schmudde https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Lingel, Imke Wilburn, Adrienne N. Hargis, Julie McAlees, Jaclyn W. Laumonnier, Yves Chougnet, Claire A. Deshmukh, Hitesh König, Peter Lewkowich, Ian P. Schmudde, Inken Prenatal antibiotics exposure does not influence experimental allergic asthma in mice |
title | Prenatal antibiotics exposure does not influence experimental allergic asthma in mice |
title_full | Prenatal antibiotics exposure does not influence experimental allergic asthma in mice |
title_fullStr | Prenatal antibiotics exposure does not influence experimental allergic asthma in mice |
title_full_unstemmed | Prenatal antibiotics exposure does not influence experimental allergic asthma in mice |
title_short | Prenatal antibiotics exposure does not influence experimental allergic asthma in mice |
title_sort | prenatal antibiotics exposure does not influence experimental allergic asthma in mice |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399857/ https://www.ncbi.nlm.nih.gov/pubmed/36032166 http://dx.doi.org/10.3389/fimmu.2022.937577 |
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