Cargando…

Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins

Cells mechanical behaviour in physiological environments is mediated by interactions with the extracellular matrix (ECM). In particular, cells can adapt their shape according to the availability of ECM proteins, e.g., fibronectin (FN). Several in vitro experiments usually simulate the ECM by functio...

Descripción completa

Detalles Bibliográficos
Autores principales: Lemma, Enrico Domenico, Jiang, Zhongxiang, Klein, Franziska, Landmann, Tanja, Weißenbruch, Kai, Bertels, Sarah, Hippler, Marc, Wehrle-Haller, Bernhard, Bastmeyer, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399860/
https://www.ncbi.nlm.nih.gov/pubmed/36032704
http://dx.doi.org/10.3389/fbioe.2022.964259
_version_ 1784772622757658624
author Lemma, Enrico Domenico
Jiang, Zhongxiang
Klein, Franziska
Landmann, Tanja
Weißenbruch, Kai
Bertels, Sarah
Hippler, Marc
Wehrle-Haller, Bernhard
Bastmeyer, Martin
author_facet Lemma, Enrico Domenico
Jiang, Zhongxiang
Klein, Franziska
Landmann, Tanja
Weißenbruch, Kai
Bertels, Sarah
Hippler, Marc
Wehrle-Haller, Bernhard
Bastmeyer, Martin
author_sort Lemma, Enrico Domenico
collection PubMed
description Cells mechanical behaviour in physiological environments is mediated by interactions with the extracellular matrix (ECM). In particular, cells can adapt their shape according to the availability of ECM proteins, e.g., fibronectin (FN). Several in vitro experiments usually simulate the ECM by functionalizing the surfaces on which cells grow with FN. However, the mechanisms underlying cell spreading on non-uniformly FN-coated two-dimensional substrates are not clarified yet. In this work, we studied cell spreading on variously functionalized substrates: FN was either uniformly distributed or selectively patterned on flat surfaces, to show that A549, BRL, B16 and NIH 3T3 cell lines are able to sense the overall FN binding sites independently of their spatial arrangement. Instead, only the total amount of available FN influences cells spreading area, which positively correlates to the FN density. Immunocytochemical analysis showed that β1 integrin subunits are mainly responsible for this behaviour, as further confirmed by spreading experiments with β1-deficient cells. In the latter case, indeed, cells areas do not show a dependency on the amount of available FN on the substrates. Therefore, we envision for β1 a predominant role in cells for sensing the number of ECM ligands with respect to other focal adhesion proteins.
format Online
Article
Text
id pubmed-9399860
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93998602022-08-25 Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins Lemma, Enrico Domenico Jiang, Zhongxiang Klein, Franziska Landmann, Tanja Weißenbruch, Kai Bertels, Sarah Hippler, Marc Wehrle-Haller, Bernhard Bastmeyer, Martin Front Bioeng Biotechnol Bioengineering and Biotechnology Cells mechanical behaviour in physiological environments is mediated by interactions with the extracellular matrix (ECM). In particular, cells can adapt their shape according to the availability of ECM proteins, e.g., fibronectin (FN). Several in vitro experiments usually simulate the ECM by functionalizing the surfaces on which cells grow with FN. However, the mechanisms underlying cell spreading on non-uniformly FN-coated two-dimensional substrates are not clarified yet. In this work, we studied cell spreading on variously functionalized substrates: FN was either uniformly distributed or selectively patterned on flat surfaces, to show that A549, BRL, B16 and NIH 3T3 cell lines are able to sense the overall FN binding sites independently of their spatial arrangement. Instead, only the total amount of available FN influences cells spreading area, which positively correlates to the FN density. Immunocytochemical analysis showed that β1 integrin subunits are mainly responsible for this behaviour, as further confirmed by spreading experiments with β1-deficient cells. In the latter case, indeed, cells areas do not show a dependency on the amount of available FN on the substrates. Therefore, we envision for β1 a predominant role in cells for sensing the number of ECM ligands with respect to other focal adhesion proteins. Frontiers Media S.A. 2022-08-10 /pmc/articles/PMC9399860/ /pubmed/36032704 http://dx.doi.org/10.3389/fbioe.2022.964259 Text en Copyright © 2022 Lemma, Jiang, Klein, Landmann, Weißenbruch, Bertels, Hippler, Wehrle-Haller and Bastmeyer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Lemma, Enrico Domenico
Jiang, Zhongxiang
Klein, Franziska
Landmann, Tanja
Weißenbruch, Kai
Bertels, Sarah
Hippler, Marc
Wehrle-Haller, Bernhard
Bastmeyer, Martin
Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins
title Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins
title_full Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins
title_fullStr Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins
title_full_unstemmed Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins
title_short Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins
title_sort adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399860/
https://www.ncbi.nlm.nih.gov/pubmed/36032704
http://dx.doi.org/10.3389/fbioe.2022.964259
work_keys_str_mv AT lemmaenricodomenico adaptationofcellspreadingtovaryingfibronectindensitiesandtopographiesisfacilitatedbyb1integrins
AT jiangzhongxiang adaptationofcellspreadingtovaryingfibronectindensitiesandtopographiesisfacilitatedbyb1integrins
AT kleinfranziska adaptationofcellspreadingtovaryingfibronectindensitiesandtopographiesisfacilitatedbyb1integrins
AT landmanntanja adaptationofcellspreadingtovaryingfibronectindensitiesandtopographiesisfacilitatedbyb1integrins
AT weißenbruchkai adaptationofcellspreadingtovaryingfibronectindensitiesandtopographiesisfacilitatedbyb1integrins
AT bertelssarah adaptationofcellspreadingtovaryingfibronectindensitiesandtopographiesisfacilitatedbyb1integrins
AT hipplermarc adaptationofcellspreadingtovaryingfibronectindensitiesandtopographiesisfacilitatedbyb1integrins
AT wehrlehallerbernhard adaptationofcellspreadingtovaryingfibronectindensitiesandtopographiesisfacilitatedbyb1integrins
AT bastmeyermartin adaptationofcellspreadingtovaryingfibronectindensitiesandtopographiesisfacilitatedbyb1integrins