Cargando…
Synergistic effects of epigallocatechin gallate and l-theanine in nerve repair and regeneration by anti-amyloid damage, promoting metabolism, and nourishing nerve cells
Green tea has significant protective activity on nerve cells, but the mechanism of action is unclear. Epigallocatechin gallate (EGCG) and N-ethyl-L-glutamine (L-theanine) are the representative functional components of green tea (Camellia sinensis). In this study, an AD model of Aβ(25–35)-induced di...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399931/ https://www.ncbi.nlm.nih.gov/pubmed/36034895 http://dx.doi.org/10.3389/fnut.2022.951415 |
_version_ | 1784772638392975360 |
---|---|
author | Xie, Xinya Wan, Juan Zheng, Xin Pan, Wenjing Yuan, Jiayi Hu, Baozhu Feng, Meiyan Liu, Zhonghua Cai, Shuxian |
author_facet | Xie, Xinya Wan, Juan Zheng, Xin Pan, Wenjing Yuan, Jiayi Hu, Baozhu Feng, Meiyan Liu, Zhonghua Cai, Shuxian |
author_sort | Xie, Xinya |
collection | PubMed |
description | Green tea has significant protective activity on nerve cells, but the mechanism of action is unclear. Epigallocatechin gallate (EGCG) and N-ethyl-L-glutamine (L-theanine) are the representative functional components of green tea (Camellia sinensis). In this study, an AD model of Aβ(25–35)-induced differentiated neural cell line PC12 cells was established to study the synergistic effect of EGCG and L-theanine in protecting neural cells. The results showed that under Aβ(25–35) stress conditions, mitochondria and axons degenerated, and the expression of cyclins was up-regulated, showing the gene and protein characteristics of cellular hyperfunction. EGCG + L-theanine inhibited inflammation and aggregate formation pathways, significantly increased the percentage of G0/G1 in the cell cycle, downregulated the expression of proteins such as p-mTOR, Cyclin D1, and Cyclin B1, upregulated the expression of GAP43, Klotho, p-AMPK, and other proteins, promoted mitochondrial activity and energy metabolism, and had repair and regeneration effects on differentiated nerve cells. The synergistic mechanism study showed that under the premise that EGCG inhibits amyloid stress and inflammation and promotes metabolism, L-theanine could play a nourish nerve effect. EGCG + L-theanine keeps differentiated nerve cells in a quiescent state, which is beneficial to the repair and regeneration of nerve cells. In addition, EGCG + L-theanine maintains the high-fidelity structure of cellular proteins. This study revealed for the first time that the synergistic effect of EGCG with L-theanine may be an effective way to promote nerve cell repair and regeneration and slow down the progression of AD. Our findings provide a new scientific basis for the relationship between tea drinking and brain protection. |
format | Online Article Text |
id | pubmed-9399931 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93999312022-08-25 Synergistic effects of epigallocatechin gallate and l-theanine in nerve repair and regeneration by anti-amyloid damage, promoting metabolism, and nourishing nerve cells Xie, Xinya Wan, Juan Zheng, Xin Pan, Wenjing Yuan, Jiayi Hu, Baozhu Feng, Meiyan Liu, Zhonghua Cai, Shuxian Front Nutr Nutrition Green tea has significant protective activity on nerve cells, but the mechanism of action is unclear. Epigallocatechin gallate (EGCG) and N-ethyl-L-glutamine (L-theanine) are the representative functional components of green tea (Camellia sinensis). In this study, an AD model of Aβ(25–35)-induced differentiated neural cell line PC12 cells was established to study the synergistic effect of EGCG and L-theanine in protecting neural cells. The results showed that under Aβ(25–35) stress conditions, mitochondria and axons degenerated, and the expression of cyclins was up-regulated, showing the gene and protein characteristics of cellular hyperfunction. EGCG + L-theanine inhibited inflammation and aggregate formation pathways, significantly increased the percentage of G0/G1 in the cell cycle, downregulated the expression of proteins such as p-mTOR, Cyclin D1, and Cyclin B1, upregulated the expression of GAP43, Klotho, p-AMPK, and other proteins, promoted mitochondrial activity and energy metabolism, and had repair and regeneration effects on differentiated nerve cells. The synergistic mechanism study showed that under the premise that EGCG inhibits amyloid stress and inflammation and promotes metabolism, L-theanine could play a nourish nerve effect. EGCG + L-theanine keeps differentiated nerve cells in a quiescent state, which is beneficial to the repair and regeneration of nerve cells. In addition, EGCG + L-theanine maintains the high-fidelity structure of cellular proteins. This study revealed for the first time that the synergistic effect of EGCG with L-theanine may be an effective way to promote nerve cell repair and regeneration and slow down the progression of AD. Our findings provide a new scientific basis for the relationship between tea drinking and brain protection. Frontiers Media S.A. 2022-08-10 /pmc/articles/PMC9399931/ /pubmed/36034895 http://dx.doi.org/10.3389/fnut.2022.951415 Text en Copyright © 2022 Xie, Wan, Zheng, Pan, Yuan, Hu, Feng, Liu and Cai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Xie, Xinya Wan, Juan Zheng, Xin Pan, Wenjing Yuan, Jiayi Hu, Baozhu Feng, Meiyan Liu, Zhonghua Cai, Shuxian Synergistic effects of epigallocatechin gallate and l-theanine in nerve repair and regeneration by anti-amyloid damage, promoting metabolism, and nourishing nerve cells |
title | Synergistic effects of epigallocatechin gallate and l-theanine in nerve repair and regeneration by anti-amyloid damage, promoting metabolism, and nourishing nerve cells |
title_full | Synergistic effects of epigallocatechin gallate and l-theanine in nerve repair and regeneration by anti-amyloid damage, promoting metabolism, and nourishing nerve cells |
title_fullStr | Synergistic effects of epigallocatechin gallate and l-theanine in nerve repair and regeneration by anti-amyloid damage, promoting metabolism, and nourishing nerve cells |
title_full_unstemmed | Synergistic effects of epigallocatechin gallate and l-theanine in nerve repair and regeneration by anti-amyloid damage, promoting metabolism, and nourishing nerve cells |
title_short | Synergistic effects of epigallocatechin gallate and l-theanine in nerve repair and regeneration by anti-amyloid damage, promoting metabolism, and nourishing nerve cells |
title_sort | synergistic effects of epigallocatechin gallate and l-theanine in nerve repair and regeneration by anti-amyloid damage, promoting metabolism, and nourishing nerve cells |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399931/ https://www.ncbi.nlm.nih.gov/pubmed/36034895 http://dx.doi.org/10.3389/fnut.2022.951415 |
work_keys_str_mv | AT xiexinya synergisticeffectsofepigallocatechingallateandltheanineinnerverepairandregenerationbyantiamyloiddamagepromotingmetabolismandnourishingnervecells AT wanjuan synergisticeffectsofepigallocatechingallateandltheanineinnerverepairandregenerationbyantiamyloiddamagepromotingmetabolismandnourishingnervecells AT zhengxin synergisticeffectsofepigallocatechingallateandltheanineinnerverepairandregenerationbyantiamyloiddamagepromotingmetabolismandnourishingnervecells AT panwenjing synergisticeffectsofepigallocatechingallateandltheanineinnerverepairandregenerationbyantiamyloiddamagepromotingmetabolismandnourishingnervecells AT yuanjiayi synergisticeffectsofepigallocatechingallateandltheanineinnerverepairandregenerationbyantiamyloiddamagepromotingmetabolismandnourishingnervecells AT hubaozhu synergisticeffectsofepigallocatechingallateandltheanineinnerverepairandregenerationbyantiamyloiddamagepromotingmetabolismandnourishingnervecells AT fengmeiyan synergisticeffectsofepigallocatechingallateandltheanineinnerverepairandregenerationbyantiamyloiddamagepromotingmetabolismandnourishingnervecells AT liuzhonghua synergisticeffectsofepigallocatechingallateandltheanineinnerverepairandregenerationbyantiamyloiddamagepromotingmetabolismandnourishingnervecells AT caishuxian synergisticeffectsofepigallocatechingallateandltheanineinnerverepairandregenerationbyantiamyloiddamagepromotingmetabolismandnourishingnervecells |