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Improving Transcriptome Fidelity Following Synovial Tissue Disaggregation

OBJECTIVE: To improve the fidelity of the cellular transcriptome of disaggregated synovial tissue for applications such as single-cell RNA sequencing (scRNAseq) by modifying the disaggregation technique. METHODS: Osteoarthritis (OA) and rheumatoid arthritis (RA) synovia were collected at arthroplast...

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Detalles Bibliográficos
Autores principales: Boyle, David L., Prideaux, Edward B., Hillman, Joshua, Wang, Wei, Firestein, Gary S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400013/
https://www.ncbi.nlm.nih.gov/pubmed/36035425
http://dx.doi.org/10.3389/fmed.2022.919748