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Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis

BACKGROUND: Bacillus subtilis, an important industrial microorganism, is commonly used in the production of industrial enzymes. Genome modification is often necessary to improve the production performance of cell. The dual-plasmid CRISPR-Cas9 system suitable for iterative genome editing has been app...

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Detalles Bibliográficos
Autores principales:	Zou, Yu, Qiu, Lu, Xie, Aowen, Han, Wenyuan, Zhang, Shangbo, Li, Jinshan, Zhao, Shumiao, Li, Yingjun, Liang, Yunxiang, Hu, Yongmei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400229/ https://www.ncbi.nlm.nih.gov/pubmed/35999638 http://dx.doi.org/10.1186/s12934-022-01896-0

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