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In vitro breast cancer models for studying mechanisms of resistance to endocrine therapy

The development of endocrine resistance is a common reason for the failure of endocrine therapies in hormone receptor-positive breast cancer. This review provides an overview of the different types of in vitro models that have been developed as tools for studying endocrine resistance. In vitro model...

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Autores principales: Cheng, Gary J., Leung, Euphemia Y., Singleton, Dean C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Open Exploration 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400723/
https://www.ncbi.nlm.nih.gov/pubmed/36045910
http://dx.doi.org/10.37349/etat.2022.00084
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author Cheng, Gary J.
Leung, Euphemia Y.
Singleton, Dean C.
author_facet Cheng, Gary J.
Leung, Euphemia Y.
Singleton, Dean C.
author_sort Cheng, Gary J.
collection PubMed
description The development of endocrine resistance is a common reason for the failure of endocrine therapies in hormone receptor-positive breast cancer. This review provides an overview of the different types of in vitro models that have been developed as tools for studying endocrine resistance. In vitro models include cell lines that have been rendered endocrine-resistant by ex vivo treatment; cell lines with de novo resistance mechanisms, including genetic alterations; three-dimensional (3D) spheroid, co-culture, and mammosphere techniques; and patient-derived organoid models. In each case, the key discoveries, different analysis strategies that are suitable, and strengths and weaknesses are discussed. Certain recently developed methodologies that can be used to further characterize the biological changes involved in endocrine resistance are then emphasized, along with a commentary on the types of research outcomes that using these techniques can support. Finally, a discussion anticipates how these recent developments will shape future trends in the field. We hope this overview will serve as a useful resource for investigators that are interested in understanding and testing hypotheses related to mechanisms of endocrine therapy resistance.
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spelling pubmed-94007232022-08-30 In vitro breast cancer models for studying mechanisms of resistance to endocrine therapy Cheng, Gary J. Leung, Euphemia Y. Singleton, Dean C. Explor Target Antitumor Ther Review The development of endocrine resistance is a common reason for the failure of endocrine therapies in hormone receptor-positive breast cancer. This review provides an overview of the different types of in vitro models that have been developed as tools for studying endocrine resistance. In vitro models include cell lines that have been rendered endocrine-resistant by ex vivo treatment; cell lines with de novo resistance mechanisms, including genetic alterations; three-dimensional (3D) spheroid, co-culture, and mammosphere techniques; and patient-derived organoid models. In each case, the key discoveries, different analysis strategies that are suitable, and strengths and weaknesses are discussed. Certain recently developed methodologies that can be used to further characterize the biological changes involved in endocrine resistance are then emphasized, along with a commentary on the types of research outcomes that using these techniques can support. Finally, a discussion anticipates how these recent developments will shape future trends in the field. We hope this overview will serve as a useful resource for investigators that are interested in understanding and testing hypotheses related to mechanisms of endocrine therapy resistance. Open Exploration 2022 2022-06-01 /pmc/articles/PMC9400723/ /pubmed/36045910 http://dx.doi.org/10.37349/etat.2022.00084 Text en © The Author(s) 2022. https://creativecommons.org/licenses/by/4.0/This is an Open Access article licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Review
Cheng, Gary J.
Leung, Euphemia Y.
Singleton, Dean C.
In vitro breast cancer models for studying mechanisms of resistance to endocrine therapy
title In vitro breast cancer models for studying mechanisms of resistance to endocrine therapy
title_full In vitro breast cancer models for studying mechanisms of resistance to endocrine therapy
title_fullStr In vitro breast cancer models for studying mechanisms of resistance to endocrine therapy
title_full_unstemmed In vitro breast cancer models for studying mechanisms of resistance to endocrine therapy
title_short In vitro breast cancer models for studying mechanisms of resistance to endocrine therapy
title_sort in vitro breast cancer models for studying mechanisms of resistance to endocrine therapy
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400723/
https://www.ncbi.nlm.nih.gov/pubmed/36045910
http://dx.doi.org/10.37349/etat.2022.00084
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