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Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy

Nanomedicine emerged some decades ago with the hope to be the solution for most unmet medical needs. However, tracking materials at nanoscale is challenging to their reduced size, below the resolution limit of most conventional techniques. In this context, we propose the use of direct stochastic opt...

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Detalles Bibliográficos
Autores principales: Riera, Roger, Tauler, Jana, Feiner‐Gracia, Natàlia, Borrós, Salvador, Fornaguera, Cristina, Albertazzi, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400995/
https://www.ncbi.nlm.nih.gov/pubmed/35212466
http://dx.doi.org/10.1002/cmdc.202100633
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author Riera, Roger
Tauler, Jana
Feiner‐Gracia, Natàlia
Borrós, Salvador
Fornaguera, Cristina
Albertazzi, Lorenzo
author_facet Riera, Roger
Tauler, Jana
Feiner‐Gracia, Natàlia
Borrós, Salvador
Fornaguera, Cristina
Albertazzi, Lorenzo
author_sort Riera, Roger
collection PubMed
description Nanomedicine emerged some decades ago with the hope to be the solution for most unmet medical needs. However, tracking materials at nanoscale is challenging to their reduced size, below the resolution limit of most conventional techniques. In this context, we propose the use of direct stochastic optical reconstruction microscopy (dSTORM) to study time stability and cell trafficking after transfection of oligopeptide end‐modified poly(β‐aminoester) (OM‐pBAE) nanoparticles. We selected different combinations of cationic end oligopeptides (arginine – R; histidine – H; and lysine – K) among polymer libraries, since the oligopeptide combination demonstrated to be useful for different applications, such as vaccination and gene silencing. We demonstrate that their time evolution as well as their cell uptake and trafficking are dependent on the oligopeptide. This study opens the pave to broad mechanistic studies at nanoscale that could enable a rational selection of specific pBAE nanoparticles composition after determining their stability and cell trafficking.
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spelling pubmed-94009952022-08-26 Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy Riera, Roger Tauler, Jana Feiner‐Gracia, Natàlia Borrós, Salvador Fornaguera, Cristina Albertazzi, Lorenzo ChemMedChem Full Papers Nanomedicine emerged some decades ago with the hope to be the solution for most unmet medical needs. However, tracking materials at nanoscale is challenging to their reduced size, below the resolution limit of most conventional techniques. In this context, we propose the use of direct stochastic optical reconstruction microscopy (dSTORM) to study time stability and cell trafficking after transfection of oligopeptide end‐modified poly(β‐aminoester) (OM‐pBAE) nanoparticles. We selected different combinations of cationic end oligopeptides (arginine – R; histidine – H; and lysine – K) among polymer libraries, since the oligopeptide combination demonstrated to be useful for different applications, such as vaccination and gene silencing. We demonstrate that their time evolution as well as their cell uptake and trafficking are dependent on the oligopeptide. This study opens the pave to broad mechanistic studies at nanoscale that could enable a rational selection of specific pBAE nanoparticles composition after determining their stability and cell trafficking. John Wiley and Sons Inc. 2022-03-18 2022-07-05 /pmc/articles/PMC9400995/ /pubmed/35212466 http://dx.doi.org/10.1002/cmdc.202100633 Text en © 2022 The Authors. ChemMedChem published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Full Papers
Riera, Roger
Tauler, Jana
Feiner‐Gracia, Natàlia
Borrós, Salvador
Fornaguera, Cristina
Albertazzi, Lorenzo
Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy
title Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy
title_full Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy
title_fullStr Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy
title_full_unstemmed Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy
title_short Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy
title_sort complex pbae nanoparticle cell trafficking: tracking both position and composition using super resolution microscopy
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400995/
https://www.ncbi.nlm.nih.gov/pubmed/35212466
http://dx.doi.org/10.1002/cmdc.202100633
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