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Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus
Optotracers are conformation‐sensitive fluorescent tracer molecules that detect peptide‐ and carbohydrate‐based biopolymers. Their binding to bacterial cell walls allows selective detection and visualisation of Staphylococcus aureus (S. aureus). Here, we investigated the structural properties provid...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400997/ https://www.ncbi.nlm.nih.gov/pubmed/35298076 http://dx.doi.org/10.1002/cbic.202100684 |
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author | Butina, Karen Lantz, Linda Choong, Ferdinand X. Tomac, Ana Shirani, Hamid Löffler, Susanne Nilsson, K. Peter R. Richter‐Dahlfors, Agneta |
author_facet | Butina, Karen Lantz, Linda Choong, Ferdinand X. Tomac, Ana Shirani, Hamid Löffler, Susanne Nilsson, K. Peter R. Richter‐Dahlfors, Agneta |
author_sort | Butina, Karen |
collection | PubMed |
description | Optotracers are conformation‐sensitive fluorescent tracer molecules that detect peptide‐ and carbohydrate‐based biopolymers. Their binding to bacterial cell walls allows selective detection and visualisation of Staphylococcus aureus (S. aureus). Here, we investigated the structural properties providing optimal detection of S. aureus. We quantified spectral shifts and fluorescence intensity in mixes of bacteria and optotracers, using automatic peak analysis, cross‐correlation, and area‐under‐curve analysis. We found that the length of the conjugated backbone and the number of charged groups, but not their distribution, are important factors for selective detection of S. aureus. The photophysical properties of optotracers were greatly improved by incorporating a donor‐acceptor‐donor (D‐A‐D)‐type motif in the conjugated backbone. With significantly reduced background and binding‐induced on‐switch of fluorescence, these optotracers enabled real‐time recordings of S. aureus growth. Collectively, this demonstrates that chemical structure and photophysics are key tunable characteristics in the development of optotracers for selective detection of bacterial species. |
format | Online Article Text |
id | pubmed-9400997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94009972022-08-26 Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus Butina, Karen Lantz, Linda Choong, Ferdinand X. Tomac, Ana Shirani, Hamid Löffler, Susanne Nilsson, K. Peter R. Richter‐Dahlfors, Agneta Chembiochem Full Papers Optotracers are conformation‐sensitive fluorescent tracer molecules that detect peptide‐ and carbohydrate‐based biopolymers. Their binding to bacterial cell walls allows selective detection and visualisation of Staphylococcus aureus (S. aureus). Here, we investigated the structural properties providing optimal detection of S. aureus. We quantified spectral shifts and fluorescence intensity in mixes of bacteria and optotracers, using automatic peak analysis, cross‐correlation, and area‐under‐curve analysis. We found that the length of the conjugated backbone and the number of charged groups, but not their distribution, are important factors for selective detection of S. aureus. The photophysical properties of optotracers were greatly improved by incorporating a donor‐acceptor‐donor (D‐A‐D)‐type motif in the conjugated backbone. With significantly reduced background and binding‐induced on‐switch of fluorescence, these optotracers enabled real‐time recordings of S. aureus growth. Collectively, this demonstrates that chemical structure and photophysics are key tunable characteristics in the development of optotracers for selective detection of bacterial species. John Wiley and Sons Inc. 2022-04-01 2022-06-03 /pmc/articles/PMC9400997/ /pubmed/35298076 http://dx.doi.org/10.1002/cbic.202100684 Text en © 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Butina, Karen Lantz, Linda Choong, Ferdinand X. Tomac, Ana Shirani, Hamid Löffler, Susanne Nilsson, K. Peter R. Richter‐Dahlfors, Agneta Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus |
title | Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus
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title_full | Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus
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title_fullStr | Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus
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title_full_unstemmed | Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus
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title_short | Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus
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title_sort | structural properties dictating selective optotracer detection of staphylococcus aureus |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400997/ https://www.ncbi.nlm.nih.gov/pubmed/35298076 http://dx.doi.org/10.1002/cbic.202100684 |
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