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Luminescent Assay for the Screening of SARS‐CoV‐2 M(Pro) Inhibitors
Since the outbreak of SARS‐CoV‐2 in December 2019 millions of infections have been reported globally. The viral chymotrypsin‐like main protease (M(Pro)) exhibits a crucial role in viral replication and represents a relevant target for antiviral drug development. In order to screen potential M(Pro) i...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9401586/ https://www.ncbi.nlm.nih.gov/pubmed/35649961 http://dx.doi.org/10.1002/cbic.202200190 |
| _version_ | 1784772996006674432 |
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| author | Sondag, Daan Merx, Jona Rossing, Emiel Boltje, Thomas J. Löwik, Dennis W. P. M. Nelissen, Frank H. T. van Geffen, Mark van 't Veer, Cornelis van Heerde, Waander L. Rutjes, Floris P. J. T. |
| author_facet | Sondag, Daan Merx, Jona Rossing, Emiel Boltje, Thomas J. Löwik, Dennis W. P. M. Nelissen, Frank H. T. van Geffen, Mark van 't Veer, Cornelis van Heerde, Waander L. Rutjes, Floris P. J. T. |
| author_sort | Sondag, Daan |
| collection | PubMed |
| description | Since the outbreak of SARS‐CoV‐2 in December 2019 millions of infections have been reported globally. The viral chymotrypsin‐like main protease (M(Pro)) exhibits a crucial role in viral replication and represents a relevant target for antiviral drug development. In order to screen potential M(Pro) inhibitors we developed a luminescent assay using a peptide based probe containing a cleavage site specific for M(Pro). This assay was validated showing IC(50) values similar to those reported in the literature for known M(Pro) inhibitors and can be used to screen new inhibitors. |
| format | Online Article Text |
| id | pubmed-9401586 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | John Wiley and Sons Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-94015862022-08-26 Luminescent Assay for the Screening of SARS‐CoV‐2 M(Pro) Inhibitors Sondag, Daan Merx, Jona Rossing, Emiel Boltje, Thomas J. Löwik, Dennis W. P. M. Nelissen, Frank H. T. van Geffen, Mark van 't Veer, Cornelis van Heerde, Waander L. Rutjes, Floris P. J. T. Chembiochem Research Articles Since the outbreak of SARS‐CoV‐2 in December 2019 millions of infections have been reported globally. The viral chymotrypsin‐like main protease (M(Pro)) exhibits a crucial role in viral replication and represents a relevant target for antiviral drug development. In order to screen potential M(Pro) inhibitors we developed a luminescent assay using a peptide based probe containing a cleavage site specific for M(Pro). This assay was validated showing IC(50) values similar to those reported in the literature for known M(Pro) inhibitors and can be used to screen new inhibitors. John Wiley and Sons Inc. 2022-06-14 2022-08-03 /pmc/articles/PMC9401586/ /pubmed/35649961 http://dx.doi.org/10.1002/cbic.202200190 Text en © 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Research Articles Sondag, Daan Merx, Jona Rossing, Emiel Boltje, Thomas J. Löwik, Dennis W. P. M. Nelissen, Frank H. T. van Geffen, Mark van 't Veer, Cornelis van Heerde, Waander L. Rutjes, Floris P. J. T. Luminescent Assay for the Screening of SARS‐CoV‐2 M(Pro) Inhibitors |
| title | Luminescent Assay for the Screening of SARS‐CoV‐2 M(Pro) Inhibitors |
| title_full | Luminescent Assay for the Screening of SARS‐CoV‐2 M(Pro) Inhibitors |
| title_fullStr | Luminescent Assay for the Screening of SARS‐CoV‐2 M(Pro) Inhibitors |
| title_full_unstemmed | Luminescent Assay for the Screening of SARS‐CoV‐2 M(Pro) Inhibitors |
| title_short | Luminescent Assay for the Screening of SARS‐CoV‐2 M(Pro) Inhibitors |
| title_sort | luminescent assay for the screening of sars‐cov‐2 m(pro) inhibitors |
| topic | Research Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9401586/ https://www.ncbi.nlm.nih.gov/pubmed/35649961 http://dx.doi.org/10.1002/cbic.202200190 |
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