Cargando…

Bufei Decoction Improves Lung-Qi Deficiency Syndrome of Chronic Obstructive Pulmonary Disease in Rats by Regulating the Balance of Th17/Treg Cells

Bufei decoction (BFD) has been applied to treat chronic obstructive pulmonary disease (COPD) for centuries as a recognized traditional Chinese herbal formula. However, mechanisms of BFD on COPD are unclear. This study conducts an inquiry into the underlying mechanisms of the therapeutic effect of BF...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Junxi, Zhu, Xing, Chen, Yunzhi, Li, Wen, Liu, Huaiquan, Chu, Cancan, Zhang, Yu, Xu, Changjun, Tong, Pingzhen, Yu, Xinran, Yang, Guangyong, Deng, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9402293/
https://www.ncbi.nlm.nih.gov/pubmed/36034952
http://dx.doi.org/10.1155/2022/1459232
_version_ 1784773142097428480
author Shen, Junxi
Zhu, Xing
Chen, Yunzhi
Li, Wen
Liu, Huaiquan
Chu, Cancan
Zhang, Yu
Xu, Changjun
Tong, Pingzhen
Yu, Xinran
Yang, Guangyong
Deng, Ying
author_facet Shen, Junxi
Zhu, Xing
Chen, Yunzhi
Li, Wen
Liu, Huaiquan
Chu, Cancan
Zhang, Yu
Xu, Changjun
Tong, Pingzhen
Yu, Xinran
Yang, Guangyong
Deng, Ying
author_sort Shen, Junxi
collection PubMed
description Bufei decoction (BFD) has been applied to treat chronic obstructive pulmonary disease (COPD) for centuries as a recognized traditional Chinese herbal formula. However, mechanisms of BFD on COPD are unclear. This study conducts an inquiry into the underlying mechanisms of the therapeutic effect of BFD on COPD. A COPD rat model with qi deficiency in lungs was established through induction using cigarette and sawdust smoking combined with intratracheal instillation of lipopolysaccharide following BFD treatment for 28 days. Changes in Th17/Treg cells of COPD rats with the syndrome of lung qi deficiency after BFD administration were verified using pulmonary function, ELISA, flow cytometry, histopathology, and Western blotting assays. The findings showed that BFD protected COPD rats from decreased lung function and lung injury. BFD administration reduced proinflammatory cytokines IL-6 and IL-17 secretion, promoted inhibitory cytokines IL-10 and TGF-β secretion, decreased Th17/Treg cell ratio, markedly downregulated the Th17 cell transcription factor ROR-γt expression, and upregulated transcription factor Foxp3 expression in Treg cells. We speculate that lung tonic soup improved pulmonary qi deficiency in rats with COPD by regulating the balance of Th17/Treg cells.
format Online
Article
Text
id pubmed-9402293
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-94022932022-08-25 Bufei Decoction Improves Lung-Qi Deficiency Syndrome of Chronic Obstructive Pulmonary Disease in Rats by Regulating the Balance of Th17/Treg Cells Shen, Junxi Zhu, Xing Chen, Yunzhi Li, Wen Liu, Huaiquan Chu, Cancan Zhang, Yu Xu, Changjun Tong, Pingzhen Yu, Xinran Yang, Guangyong Deng, Ying Evid Based Complement Alternat Med Research Article Bufei decoction (BFD) has been applied to treat chronic obstructive pulmonary disease (COPD) for centuries as a recognized traditional Chinese herbal formula. However, mechanisms of BFD on COPD are unclear. This study conducts an inquiry into the underlying mechanisms of the therapeutic effect of BFD on COPD. A COPD rat model with qi deficiency in lungs was established through induction using cigarette and sawdust smoking combined with intratracheal instillation of lipopolysaccharide following BFD treatment for 28 days. Changes in Th17/Treg cells of COPD rats with the syndrome of lung qi deficiency after BFD administration were verified using pulmonary function, ELISA, flow cytometry, histopathology, and Western blotting assays. The findings showed that BFD protected COPD rats from decreased lung function and lung injury. BFD administration reduced proinflammatory cytokines IL-6 and IL-17 secretion, promoted inhibitory cytokines IL-10 and TGF-β secretion, decreased Th17/Treg cell ratio, markedly downregulated the Th17 cell transcription factor ROR-γt expression, and upregulated transcription factor Foxp3 expression in Treg cells. We speculate that lung tonic soup improved pulmonary qi deficiency in rats with COPD by regulating the balance of Th17/Treg cells. Hindawi 2022-08-17 /pmc/articles/PMC9402293/ /pubmed/36034952 http://dx.doi.org/10.1155/2022/1459232 Text en Copyright © 2022 Junxi Shen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shen, Junxi
Zhu, Xing
Chen, Yunzhi
Li, Wen
Liu, Huaiquan
Chu, Cancan
Zhang, Yu
Xu, Changjun
Tong, Pingzhen
Yu, Xinran
Yang, Guangyong
Deng, Ying
Bufei Decoction Improves Lung-Qi Deficiency Syndrome of Chronic Obstructive Pulmonary Disease in Rats by Regulating the Balance of Th17/Treg Cells
title Bufei Decoction Improves Lung-Qi Deficiency Syndrome of Chronic Obstructive Pulmonary Disease in Rats by Regulating the Balance of Th17/Treg Cells
title_full Bufei Decoction Improves Lung-Qi Deficiency Syndrome of Chronic Obstructive Pulmonary Disease in Rats by Regulating the Balance of Th17/Treg Cells
title_fullStr Bufei Decoction Improves Lung-Qi Deficiency Syndrome of Chronic Obstructive Pulmonary Disease in Rats by Regulating the Balance of Th17/Treg Cells
title_full_unstemmed Bufei Decoction Improves Lung-Qi Deficiency Syndrome of Chronic Obstructive Pulmonary Disease in Rats by Regulating the Balance of Th17/Treg Cells
title_short Bufei Decoction Improves Lung-Qi Deficiency Syndrome of Chronic Obstructive Pulmonary Disease in Rats by Regulating the Balance of Th17/Treg Cells
title_sort bufei decoction improves lung-qi deficiency syndrome of chronic obstructive pulmonary disease in rats by regulating the balance of th17/treg cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9402293/
https://www.ncbi.nlm.nih.gov/pubmed/36034952
http://dx.doi.org/10.1155/2022/1459232
work_keys_str_mv AT shenjunxi bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT zhuxing bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT chenyunzhi bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT liwen bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT liuhuaiquan bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT chucancan bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT zhangyu bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT xuchangjun bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT tongpingzhen bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT yuxinran bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT yangguangyong bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells
AT dengying bufeidecoctionimproveslungqideficiencysyndromeofchronicobstructivepulmonarydiseaseinratsbyregulatingthebalanceofth17tregcells