Cargando…

In vitro assessment of pancreatic hormone secretion from isolated porcine islets

The potential use of porcine islets for transplantation in humans has triggered interest in understanding porcine islet physiology. However, the number of studies dedicated to this topic has remained limited, as most islet physiologists prefer to use the less time-consuming rodent model or the more...

Descripción completa

Detalles Bibliográficos
Autores principales: Mourad, Nizar I., Xhema, Daela, Gianello, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9402940/
https://www.ncbi.nlm.nih.gov/pubmed/36034433
http://dx.doi.org/10.3389/fendo.2022.935060
_version_ 1784773256505458688
author Mourad, Nizar I.
Xhema, Daela
Gianello, Pierre
author_facet Mourad, Nizar I.
Xhema, Daela
Gianello, Pierre
author_sort Mourad, Nizar I.
collection PubMed
description The potential use of porcine islets for transplantation in humans has triggered interest in understanding porcine islet physiology. However, the number of studies dedicated to this topic has remained limited, as most islet physiologists prefer to use the less time-consuming rodent model or the more clinically relevant human islet. An often-overlooked aspect of pig islet physiology is its alpha cell activity and regulation of its glucagon secretion. In vitro islet perifusion is a reliable method to study the dynamics of hormone secretion in response to different stimuli. We thus used this method to quantify and study glucagon secretion from pig islets. Pancreatic islets were isolated from 20 neonatal (14 to 21-day old) and 5 adult (>2 years) pigs and cultured in appropriate media. Islet perifusion experiments were performed 8 to 10 days post-isolation for neonatal islets and 1 to 2 days post-isolation for adult islets. Insulin and glucagon were quantified in perifusion effluent fractions as well as in islet extracts by RIA. Increasing glucose concentration from 1 mM to 15 mM markedly inhibited glucagon secretion independently of animal age. Interestingly, the effect of high glucose was more drastic on glucagon secretion compared to its effect on insulin secretion. In vivo, glucose injection during IVGTT initiated a quick (2-10 minutes) 3-fold decrease of plasmatic glucagon whereas the increase of plasmatic insulin took 20 minutes to become significant. These results suggest that regulation of glucagon secretion significantly contributes to glucose homeostasis in pigs and might compensate for the mild changes in insulin secretion in response to changes in glucose concentration.
format Online
Article
Text
id pubmed-9402940
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-94029402022-08-26 In vitro assessment of pancreatic hormone secretion from isolated porcine islets Mourad, Nizar I. Xhema, Daela Gianello, Pierre Front Endocrinol (Lausanne) Endocrinology The potential use of porcine islets for transplantation in humans has triggered interest in understanding porcine islet physiology. However, the number of studies dedicated to this topic has remained limited, as most islet physiologists prefer to use the less time-consuming rodent model or the more clinically relevant human islet. An often-overlooked aspect of pig islet physiology is its alpha cell activity and regulation of its glucagon secretion. In vitro islet perifusion is a reliable method to study the dynamics of hormone secretion in response to different stimuli. We thus used this method to quantify and study glucagon secretion from pig islets. Pancreatic islets were isolated from 20 neonatal (14 to 21-day old) and 5 adult (>2 years) pigs and cultured in appropriate media. Islet perifusion experiments were performed 8 to 10 days post-isolation for neonatal islets and 1 to 2 days post-isolation for adult islets. Insulin and glucagon were quantified in perifusion effluent fractions as well as in islet extracts by RIA. Increasing glucose concentration from 1 mM to 15 mM markedly inhibited glucagon secretion independently of animal age. Interestingly, the effect of high glucose was more drastic on glucagon secretion compared to its effect on insulin secretion. In vivo, glucose injection during IVGTT initiated a quick (2-10 minutes) 3-fold decrease of plasmatic glucagon whereas the increase of plasmatic insulin took 20 minutes to become significant. These results suggest that regulation of glucagon secretion significantly contributes to glucose homeostasis in pigs and might compensate for the mild changes in insulin secretion in response to changes in glucose concentration. Frontiers Media S.A. 2022-08-11 /pmc/articles/PMC9402940/ /pubmed/36034433 http://dx.doi.org/10.3389/fendo.2022.935060 Text en Copyright © 2022 Mourad, Xhema and Gianello https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Mourad, Nizar I.
Xhema, Daela
Gianello, Pierre
In vitro assessment of pancreatic hormone secretion from isolated porcine islets
title In vitro assessment of pancreatic hormone secretion from isolated porcine islets
title_full In vitro assessment of pancreatic hormone secretion from isolated porcine islets
title_fullStr In vitro assessment of pancreatic hormone secretion from isolated porcine islets
title_full_unstemmed In vitro assessment of pancreatic hormone secretion from isolated porcine islets
title_short In vitro assessment of pancreatic hormone secretion from isolated porcine islets
title_sort in vitro assessment of pancreatic hormone secretion from isolated porcine islets
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9402940/
https://www.ncbi.nlm.nih.gov/pubmed/36034433
http://dx.doi.org/10.3389/fendo.2022.935060
work_keys_str_mv AT mouradnizari invitroassessmentofpancreatichormonesecretionfromisolatedporcineislets
AT xhemadaela invitroassessmentofpancreatichormonesecretionfromisolatedporcineislets
AT gianellopierre invitroassessmentofpancreatichormonesecretionfromisolatedporcineislets