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Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects

To disclose the net effect of light on microalgal growth in photobioreactors, self-shading and mixing-induced light–dark cycles must be minimized and discerned from the transient phenomena of acclimation. In this work, we performed experiments of continuous microalgal cultivation in small-scale phot...

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Autores principales: Saccardo, Alberto, Bezzo, Fabrizio, Sforza, Eleonora
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9402969/
https://www.ncbi.nlm.nih.gov/pubmed/36032730
http://dx.doi.org/10.3389/fbioe.2022.977429
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author Saccardo, Alberto
Bezzo, Fabrizio
Sforza, Eleonora
author_facet Saccardo, Alberto
Bezzo, Fabrizio
Sforza, Eleonora
author_sort Saccardo, Alberto
collection PubMed
description To disclose the net effect of light on microalgal growth in photobioreactors, self-shading and mixing-induced light–dark cycles must be minimized and discerned from the transient phenomena of acclimation. In this work, we performed experiments of continuous microalgal cultivation in small-scale photobioreactors with different thicknesses (from 2 to 35 mm): working at a steady state allowed us to describe the effect of light after acclimation, while the geometry of the reactor was adjusted to find the threshold light path that can discriminate different phenomena. Experiments showed an increased inhibition under smaller culture light paths, suggesting a strong shading effect at thicknesses higher than 8 mm where mixing-induced light–dark cycles may occur. A Haldane-like model was applied and kinetic parameters retrieved, showing possible issues in the scalability of experimental results at different light paths if mixing-induced light–dark cycles are not considered. To further highlight the influence of mixing cycles, we proposed an analogy between small-scale operations with continuous light and PBR operations with pulsed light, with the computation of characteristic parameters from pulsed-light microalgae growth mathematical modeling.
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spelling pubmed-94029692022-08-26 Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects Saccardo, Alberto Bezzo, Fabrizio Sforza, Eleonora Front Bioeng Biotechnol Bioengineering and Biotechnology To disclose the net effect of light on microalgal growth in photobioreactors, self-shading and mixing-induced light–dark cycles must be minimized and discerned from the transient phenomena of acclimation. In this work, we performed experiments of continuous microalgal cultivation in small-scale photobioreactors with different thicknesses (from 2 to 35 mm): working at a steady state allowed us to describe the effect of light after acclimation, while the geometry of the reactor was adjusted to find the threshold light path that can discriminate different phenomena. Experiments showed an increased inhibition under smaller culture light paths, suggesting a strong shading effect at thicknesses higher than 8 mm where mixing-induced light–dark cycles may occur. A Haldane-like model was applied and kinetic parameters retrieved, showing possible issues in the scalability of experimental results at different light paths if mixing-induced light–dark cycles are not considered. To further highlight the influence of mixing cycles, we proposed an analogy between small-scale operations with continuous light and PBR operations with pulsed light, with the computation of characteristic parameters from pulsed-light microalgae growth mathematical modeling. Frontiers Media S.A. 2022-08-11 /pmc/articles/PMC9402969/ /pubmed/36032730 http://dx.doi.org/10.3389/fbioe.2022.977429 Text en Copyright © 2022 Saccardo, Bezzo and Sforza. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Saccardo, Alberto
Bezzo, Fabrizio
Sforza, Eleonora
Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects
title Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects
title_full Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects
title_fullStr Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects
title_full_unstemmed Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects
title_short Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects
title_sort microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9402969/
https://www.ncbi.nlm.nih.gov/pubmed/36032730
http://dx.doi.org/10.3389/fbioe.2022.977429
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