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Improving human papillomavirus (HPV) testing in the cervical cancer elimination era: The 2021 HPV LabNet international proficiency study

BACKGROUND: Proficient Human Papillomavirus (HPV) genotyping services are essential to support HPV and cervical cancer elimination strategies, in particular to support HPV vaccine research. OBJECTIVES: To perform a global HPV genotyping proficiency study, with evaluation in relation to previous prof...

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Detalles Bibliográficos
Autores principales: Arroyo Mühr, Laila Sara, Eklund, Carina, Lagheden, Camilla, Forslund, Ola, Robertsson, Karin Dahlin, Dillner, Joakim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9402991/
https://www.ncbi.nlm.nih.gov/pubmed/35820294
http://dx.doi.org/10.1016/j.jcv.2022.105237
Descripción
Sumario:BACKGROUND: Proficient Human Papillomavirus (HPV) genotyping services are essential to support HPV and cervical cancer elimination strategies, in particular to support HPV vaccine research. OBJECTIVES: To perform a global HPV genotyping proficiency study, with evaluation in relation to previous proficiency studies. STUDY DESIGN: The proficiency panel contained 44 coded samples (40 samples containing one or more purified HPV types (HPV6/11/16/18/31/33/35/39/45/51/52/56/58/59/68a/68b) in human DNA, 1 human DNA control and 3 DNA extraction controls). Proficiency required detection of both single and multiple infections of 50 International Units of HPV 16/18, of 500 genome equivalents for other HPV types and no false positivity. RESULTS: One hundred and thirty-two laboratories submitted 211 datasets. Most assays used (182/211 datasets) were commercially available. An all-time high of 75% of the datasets were 100% proficient. One or more false positives were found in 17.5% of datasets. Among laboratories who participated in the 2019 proficiency study, full proficiency increased from 25% in 2019 to 60% in 2021. The high overall proficiency was mostly attributable to a large number of new laboratories, which used similar assays. CONCLUSIONS: The worldwide deterioration in comparability and reliability of HPV testing found in 2019 is now reversed and an overall increase in proficiency is found.