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Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency

Platelets play a crucial role in the recruitment of neutrophils, mediated by P-selectin, CCL5, and ICAM-2. In this study, we prepared platelet membrane nanovesicles from activated platelets. Whether activated platelet membrane nanovesicles can recruit neutrophils has not been reported, nor has their...

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Autores principales: Shang, Yinghui, Sun, Juntao, Wu, Xin, Wang, Qinghai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403082/
https://www.ncbi.nlm.nih.gov/pubmed/36034656
http://dx.doi.org/10.3389/fchem.2022.955995
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author Shang, Yinghui
Sun, Juntao
Wu, Xin
Wang, Qinghai
author_facet Shang, Yinghui
Sun, Juntao
Wu, Xin
Wang, Qinghai
author_sort Shang, Yinghui
collection PubMed
description Platelets play a crucial role in the recruitment of neutrophils, mediated by P-selectin, CCL5, and ICAM-2. In this study, we prepared platelet membrane nanovesicles from activated platelets. Whether activated platelet membrane nanovesicles can recruit neutrophils has not been reported, nor has their role in antitumor immunity. The results of SDS-PAGE showed that the platelet membrane nanovesicles retained almost all the proteins of platelets. Western blotting showed that both the activated platelets and the platelet membrane nanovesicles expressed P-selectin, ICAM-2, and CCL5. In vivo results of a mouse model of breast cancer-transplanted tumor showed that tumor volume reduced significantly, Ki-67-positive tumor cells decreased, and TUNEL-positive tumor cells increased in tumors after treatment with activated platelet membrane nanovesicles (aPNs). After treatment with aPNs, not only the number of neutrophils, CD8(+), CD4(+) T cells, and B cells increased, but also IL-12, TNF-α, and IFN-γ levels elevated significantly in tumor tissues.
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spelling pubmed-94030822022-08-26 Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency Shang, Yinghui Sun, Juntao Wu, Xin Wang, Qinghai Front Chem Chemistry Platelets play a crucial role in the recruitment of neutrophils, mediated by P-selectin, CCL5, and ICAM-2. In this study, we prepared platelet membrane nanovesicles from activated platelets. Whether activated platelet membrane nanovesicles can recruit neutrophils has not been reported, nor has their role in antitumor immunity. The results of SDS-PAGE showed that the platelet membrane nanovesicles retained almost all the proteins of platelets. Western blotting showed that both the activated platelets and the platelet membrane nanovesicles expressed P-selectin, ICAM-2, and CCL5. In vivo results of a mouse model of breast cancer-transplanted tumor showed that tumor volume reduced significantly, Ki-67-positive tumor cells decreased, and TUNEL-positive tumor cells increased in tumors after treatment with activated platelet membrane nanovesicles (aPNs). After treatment with aPNs, not only the number of neutrophils, CD8(+), CD4(+) T cells, and B cells increased, but also IL-12, TNF-α, and IFN-γ levels elevated significantly in tumor tissues. Frontiers Media S.A. 2022-08-11 /pmc/articles/PMC9403082/ /pubmed/36034656 http://dx.doi.org/10.3389/fchem.2022.955995 Text en Copyright © 2022 Shang, Sun, Wu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Shang, Yinghui
Sun, Juntao
Wu, Xin
Wang, Qinghai
Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency
title Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency
title_full Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency
title_fullStr Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency
title_full_unstemmed Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency
title_short Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency
title_sort activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403082/
https://www.ncbi.nlm.nih.gov/pubmed/36034656
http://dx.doi.org/10.3389/fchem.2022.955995
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