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Phaseolus vulgaris extract ameliorates high-fat diet-induced colonic barrier dysfunction and inflammation in mice by regulating peroxisome proliferator-activated receptor expression and butyrate levels

Obesity is a health concern worldwide, and its onset is multifactorial. In addition to metabolic syndrome, a high-fat diet induces many deleterious downstream effects, such as chronic systemic inflammation, a loss of gut barrier integrity, and gut microbial dysbiosis, with a reduction of many butyra...

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Autores principales: Avagliano, Carmen, De Caro, Carmen, Cuozzo, Mariarosaria, Liguori, Fabrizio Maria, La Rana, Giovanna, Micheli, Laura, Di Cesare Mannelli, Lorenzo, Ghelardini, Carla, Paciello, Orlando, Russo, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403263/
https://www.ncbi.nlm.nih.gov/pubmed/36034787
http://dx.doi.org/10.3389/fphar.2022.930832
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author Avagliano, Carmen
De Caro, Carmen
Cuozzo, Mariarosaria
Liguori, Fabrizio Maria
La Rana, Giovanna
Micheli, Laura
Di Cesare Mannelli, Lorenzo
Ghelardini, Carla
Paciello, Orlando
Russo, Roberto
author_facet Avagliano, Carmen
De Caro, Carmen
Cuozzo, Mariarosaria
Liguori, Fabrizio Maria
La Rana, Giovanna
Micheli, Laura
Di Cesare Mannelli, Lorenzo
Ghelardini, Carla
Paciello, Orlando
Russo, Roberto
author_sort Avagliano, Carmen
collection PubMed
description Obesity is a health concern worldwide, and its onset is multifactorial. In addition to metabolic syndrome, a high-fat diet induces many deleterious downstream effects, such as chronic systemic inflammation, a loss of gut barrier integrity, and gut microbial dysbiosis, with a reduction of many butyrate-producing bacteria. These conditions can be ameliorated by increasing legumes in the daily diet. White and kidney beans (Phaseolus vulgaris L.) and their non-nutritive bioactive component phaseolamin were demonstrated to mitigate several pathological features related to a metabolic syndrome-like condition. The aim of the present study was to investigate the molecular pathways involved in the protective effects on the intestinal and liver environment of a chronic oral treatment with P. vulgaris extract (PHAS) on a murine model of the high-fat diet. Results show that PHAS treatment has an anti-inflammatory effect on the liver, colon, and cecum. This protective effect was mediated by peroxisome proliferator-activated receptor (PPAR)-α and γ. Moreover, we also observed that repeated PHAS treatment was able to restore tight junctions’ expression and protective factors of colon and cecum integrity disrupted in HFD mice. This improvement was correlated with a significant increase of butyrate levels in serum and fecal samples compared to the HFD group. These data underline that prolonged treatment with PHAS significantly reduces some pathological features related to the metabolic syndrome-like condition, such as inflammation and intestinal barrier disruption; therefore, PHAS could be a valid tool to be associated with the therapeutic strategy.
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spelling pubmed-94032632022-08-26 Phaseolus vulgaris extract ameliorates high-fat diet-induced colonic barrier dysfunction and inflammation in mice by regulating peroxisome proliferator-activated receptor expression and butyrate levels Avagliano, Carmen De Caro, Carmen Cuozzo, Mariarosaria Liguori, Fabrizio Maria La Rana, Giovanna Micheli, Laura Di Cesare Mannelli, Lorenzo Ghelardini, Carla Paciello, Orlando Russo, Roberto Front Pharmacol Pharmacology Obesity is a health concern worldwide, and its onset is multifactorial. In addition to metabolic syndrome, a high-fat diet induces many deleterious downstream effects, such as chronic systemic inflammation, a loss of gut barrier integrity, and gut microbial dysbiosis, with a reduction of many butyrate-producing bacteria. These conditions can be ameliorated by increasing legumes in the daily diet. White and kidney beans (Phaseolus vulgaris L.) and their non-nutritive bioactive component phaseolamin were demonstrated to mitigate several pathological features related to a metabolic syndrome-like condition. The aim of the present study was to investigate the molecular pathways involved in the protective effects on the intestinal and liver environment of a chronic oral treatment with P. vulgaris extract (PHAS) on a murine model of the high-fat diet. Results show that PHAS treatment has an anti-inflammatory effect on the liver, colon, and cecum. This protective effect was mediated by peroxisome proliferator-activated receptor (PPAR)-α and γ. Moreover, we also observed that repeated PHAS treatment was able to restore tight junctions’ expression and protective factors of colon and cecum integrity disrupted in HFD mice. This improvement was correlated with a significant increase of butyrate levels in serum and fecal samples compared to the HFD group. These data underline that prolonged treatment with PHAS significantly reduces some pathological features related to the metabolic syndrome-like condition, such as inflammation and intestinal barrier disruption; therefore, PHAS could be a valid tool to be associated with the therapeutic strategy. Frontiers Media S.A. 2022-08-11 /pmc/articles/PMC9403263/ /pubmed/36034787 http://dx.doi.org/10.3389/fphar.2022.930832 Text en Copyright © 2022 Avagliano, De Caro, Cuozzo, Liguori, La Rana, Micheli, Di Cesare Mannelli, Ghelardini, Paciello and Russo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Avagliano, Carmen
De Caro, Carmen
Cuozzo, Mariarosaria
Liguori, Fabrizio Maria
La Rana, Giovanna
Micheli, Laura
Di Cesare Mannelli, Lorenzo
Ghelardini, Carla
Paciello, Orlando
Russo, Roberto
Phaseolus vulgaris extract ameliorates high-fat diet-induced colonic barrier dysfunction and inflammation in mice by regulating peroxisome proliferator-activated receptor expression and butyrate levels
title Phaseolus vulgaris extract ameliorates high-fat diet-induced colonic barrier dysfunction and inflammation in mice by regulating peroxisome proliferator-activated receptor expression and butyrate levels
title_full Phaseolus vulgaris extract ameliorates high-fat diet-induced colonic barrier dysfunction and inflammation in mice by regulating peroxisome proliferator-activated receptor expression and butyrate levels
title_fullStr Phaseolus vulgaris extract ameliorates high-fat diet-induced colonic barrier dysfunction and inflammation in mice by regulating peroxisome proliferator-activated receptor expression and butyrate levels
title_full_unstemmed Phaseolus vulgaris extract ameliorates high-fat diet-induced colonic barrier dysfunction and inflammation in mice by regulating peroxisome proliferator-activated receptor expression and butyrate levels
title_short Phaseolus vulgaris extract ameliorates high-fat diet-induced colonic barrier dysfunction and inflammation in mice by regulating peroxisome proliferator-activated receptor expression and butyrate levels
title_sort phaseolus vulgaris extract ameliorates high-fat diet-induced colonic barrier dysfunction and inflammation in mice by regulating peroxisome proliferator-activated receptor expression and butyrate levels
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403263/
https://www.ncbi.nlm.nih.gov/pubmed/36034787
http://dx.doi.org/10.3389/fphar.2022.930832
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